Demethylation of the HACE1 gene promoter inhibits the proliferation of human liver cancer cells

  • Authors:
    • Zhijun Yu
    • Yinyin Li
    • Tao Han
    • Zhaozhe Liu
  • View Affiliations

  • Published online on: March 12, 2019     https://doi.org/10.3892/ol.2019.10139
  • Pages: 4361-4368
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Abstract

HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1) is frequently downregulated or lost in numerous types of cancer, including liver cancer. The aim of the present study was to examine whether demethylation of the HACE1 gene could inhibit tumour progression. The expression of HACE1 was detected in liver cancer cell lines. Clustered regularly interspaced short palindromic repeats (CRISPR)‑CRISPR‑associated (Cas)‑based demethylation single guide RNAs for the HACE1 gene promoter were designed and transfected into liver cancer cells. Subsequently, proliferation was detected by MTT and colony formation assays, and optineurin (OPTN) ubiquitination and microtubule‑associated proteins 1A/1B light chain 3B protein levels were detected by immunoblotting. The levels of HACE1 were significantly reduced in liver cancer cell lines compared with in a normal liver cell line. Demethylation of the HACE1 gene promoter increased HACE1 expression, inhibited the proliferation of liver cancer cells, and promoted OPTN ubiquitination and autophagy activity in liver cancer cells. In conclusion, activation of HACE1 expression by promoter demethylation may provide a suitable approach for anticancer therapy.
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May-2019
Volume 17 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Yu Z, Li Y, Han T and Liu Z: Demethylation of the HACE1 gene promoter inhibits the proliferation of human liver cancer cells. Oncol Lett 17: 4361-4368, 2019.
APA
Yu, Z., Li, Y., Han, T., & Liu, Z. (2019). Demethylation of the HACE1 gene promoter inhibits the proliferation of human liver cancer cells. Oncology Letters, 17, 4361-4368. https://doi.org/10.3892/ol.2019.10139
MLA
Yu, Z., Li, Y., Han, T., Liu, Z."Demethylation of the HACE1 gene promoter inhibits the proliferation of human liver cancer cells". Oncology Letters 17.5 (2019): 4361-4368.
Chicago
Yu, Z., Li, Y., Han, T., Liu, Z."Demethylation of the HACE1 gene promoter inhibits the proliferation of human liver cancer cells". Oncology Letters 17, no. 5 (2019): 4361-4368. https://doi.org/10.3892/ol.2019.10139