Open Access

Investigation of miRNA‑ and lncRNA‑mediated competing endogenous RNA network in cholangiocarcinoma

  • Authors:
    • Yanxin He
    • Chao Liu
    • Pan Song
    • Zhigang Pang
    • Zhuomao Mo
    • Chuiguo Huang
    • Tingting Yan
    • Meng Sun
    • Xianen Fa
  • View Affiliations

  • Published online on: September 12, 2019     https://doi.org/10.3892/ol.2019.10852
  • Pages: 5283-5293
  • Copyright: © He et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cholangiocarcinoma (CCA) is a biliary malignancy which is prone to lymphatic metastasis and has a high mortality rate. This disease lacks effective therapeutic targets and prognostic molecular biomarkers. The aim of the current study was to investigate differentially expressed genes and elucidate their association with CCA and the underlying mechanisms of action. mRNAs, long non‑coding RNAs (lncRNAs) and microRNAs (miRNAs) obtained from 36 CCA samples and nine normal samples from The Cancer Genome Atlas were integrated. Subsequently, 1,095 differentially expressed (DE) mRNAs and 75 DE miRNAs were identified using a threshold of |log2 fold change|>2 and an adjusted P<0.01. Weighted gene co‑expression network analysis was used to identify the DEmRNAs that could be key target genes in CCA. A total of 12 hub DEmRNAs were identified as targetable genes. Furthermore, the hub DEmRNAs‑DElncRNAs pairs were identified using the miRTarBase and miRcode databases. Cytoscape software was used to construct and visualize the protein‑protein interactions and the competing endogenous RNA network. Survival time analysis and correlation analysis were used to further evaluate the hub genes. The results obtained in the current study suggested that spalt like transcription factor 3 and OPCML intronic transcript 1 may serve an important role in the development and progression of CCA.
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November-2019
Volume 18 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
He Y, Liu C, Song P, Pang Z, Mo Z, Huang C, Yan T, Sun M and Fa X: Investigation of miRNA‑ and lncRNA‑mediated competing endogenous RNA network in cholangiocarcinoma. Oncol Lett 18: 5283-5293, 2019.
APA
He, Y., Liu, C., Song, P., Pang, Z., Mo, Z., Huang, C. ... Fa, X. (2019). Investigation of miRNA‑ and lncRNA‑mediated competing endogenous RNA network in cholangiocarcinoma. Oncology Letters, 18, 5283-5293. https://doi.org/10.3892/ol.2019.10852
MLA
He, Y., Liu, C., Song, P., Pang, Z., Mo, Z., Huang, C., Yan, T., Sun, M., Fa, X."Investigation of miRNA‑ and lncRNA‑mediated competing endogenous RNA network in cholangiocarcinoma". Oncology Letters 18.5 (2019): 5283-5293.
Chicago
He, Y., Liu, C., Song, P., Pang, Z., Mo, Z., Huang, C., Yan, T., Sun, M., Fa, X."Investigation of miRNA‑ and lncRNA‑mediated competing endogenous RNA network in cholangiocarcinoma". Oncology Letters 18, no. 5 (2019): 5283-5293. https://doi.org/10.3892/ol.2019.10852