MicroRNA‑663b enhances migration and invasion by targeting adenomatous polyposis coli 2 in colorectal carcinoma cells

Xiao,Fenqiang; Chen,Wangbin; Yu,Chao; Zhao,Gang

doi:10.3892/ol.2020.11482

MicroRNA‑663b enhances migration and invasion by targeting adenomatous polyposis coli 2 in colorectal carcinoma cells

Authors:
- Fenqiang Xiao
- Wangbin Chen
- Chao Yu
- Gang Zhao
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Affiliations: Department of Emergency Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China, Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China, Department of Medical Laboratory, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China
Published online on: March 27, 2020 https://doi.org/10.3892/ol.2020.11482
Pages: 3701-3710
Copyright: © Xiao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Colorectal carcinoma (CRC) is one of the leading causes of cancer-associated mortality worldwide. Dysregulation of microRNA (miR)‑663b has been reported in a variety of diseases. However, the specific biological function of miR‑663b in CRC requires further investigation. The aim of the present study was to elucidate the role and underlying molecular mechanism of action of miR‑663b in CRC. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) analysis and western blot analysis were employed to measure the expression of miR‑663b at the RNA and protein level, respectively. Flow cytometry was used to detect cell apoptosis. Cell proliferation, migration and invasion were evaluated by the Cell Counting Kit‑8, wound healing and Transwell assays, respectively. A dual‑luciferase reporter assay was used to validate the potential target gene of miR‑663b. The expression of miR‑663b was identified to be markedly upregulated in CRC cells. Ectopic miR‑663b expression promoted CRC cell proliferation, migration and invasion, and inhibited apoptosis. The dual‑luciferase reporter assay identified adenomatous polyposis coli 2 (APC2) as a direct target of miR‑663b in CRC cells. Further investigation indicated that miR‑663b was involved in CRC cell invasion through the Wnt/β‑catenin pathway. Therefore, overexpression of miR‑663b was able to promote CRC cell proliferation, migration and invasion by regulating the Wnt/β‑catenin pathway through targeting APC2, suggesting that miR‑663b may be a useful target for the diagnosis and treatment of CRC.

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