Open Access

Integrated analysis of microRNA and transcription factors in the bone marrow of patients with acute monocytic leukemia

  • Authors:
    • Xiao-Cong Lin
    • Qin Yang
    • Wei-Yu Fu
    • Liu-Bo Lan
    • Hang Ding
    • Yu-Ming Zhang
    • Ning Li
    • Hai-Tao Zhang
  • View Affiliations

  • Published online on: November 18, 2020     https://doi.org/10.3892/ol.2020.12311
  • Article Number: 50
  • Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Acutemonocytic leukemia (AMoL) is a distinct subtype of acute myeloid leukemia (AML) with poor prognosis. However, the molecular mechanisms and key regulators involved in the global regulation of gene expression levels in AMoL are poorly understood. In order to elucidate the role of microRNAs (miRNAs/miRs) and transcription factors (TFs) in AMoL pathogenesis at the network level, miRNA and TF expression level profiles were systematically analyzed by miRNA sequencing and TF array, respectively; this identified 285 differentially expressed miRNAs and 139 differentially expressed TFs in AMoL samples compared with controls. By combining expression level profile data and bioinformatics tools available for predicting TF and miRNA targets, a comprehensive AMoL‑specific miRNA‑TF‑mediated regulatory network was constructed. A total of 26 miRNAs and 23 TFs were identified as hub nodes in the network. Among these hubs, miR‑29b‑3p, MYC, TP53 and NFKB1 were determined to be potential AMoL regulators, and were subsequently extracted to construct sub‑networks. A hypothetical pathway model was also proposed for miR‑29b‑3p to reveal the potential co‑regulatory mechanisms of miR‑29b‑3p, MYC, TP53 and NFKB1 in AMoL. The present study provided an effective approach to discover critical regulators via a comprehensive regulatory network in AMoL, in addition to enhancing understanding of the pathogenesis of this disease at the molecular level.
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January-2021
Volume 21 Issue 1

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Copy and paste a formatted citation
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Spandidos Publications style
Lin X, Yang Q, Fu W, Lan L, Ding H, Zhang Y, Li N and Zhang H: Integrated analysis of microRNA and transcription factors in the bone marrow of patients with acute monocytic leukemia. Oncol Lett 21: 50, 2021.
APA
Lin, X., Yang, Q., Fu, W., Lan, L., Ding, H., Zhang, Y. ... Zhang, H. (2021). Integrated analysis of microRNA and transcription factors in the bone marrow of patients with acute monocytic leukemia. Oncology Letters, 21, 50. https://doi.org/10.3892/ol.2020.12311
MLA
Lin, X., Yang, Q., Fu, W., Lan, L., Ding, H., Zhang, Y., Li, N., Zhang, H."Integrated analysis of microRNA and transcription factors in the bone marrow of patients with acute monocytic leukemia". Oncology Letters 21.1 (2021): 50.
Chicago
Lin, X., Yang, Q., Fu, W., Lan, L., Ding, H., Zhang, Y., Li, N., Zhang, H."Integrated analysis of microRNA and transcription factors in the bone marrow of patients with acute monocytic leukemia". Oncology Letters 21, no. 1 (2021): 50. https://doi.org/10.3892/ol.2020.12311