Aqueous extracts of <em>Sanghuangporus vaninii</em> induce S‑phase arrest and apoptosis in human melanoma A375 cells

Yu,Taihen; Zhong,Shi; Sun,Yuqing; Sun,Haiyan; Chen,Weiguo; Li,Yougui; Zhu,Jianxun; Lu,Longxi; Huo,Jinxi

doi:10.3892/ol.2021.12889

Aqueous extracts of Sanghuangporus vaninii induce S‑phase arrest and apoptosis in human melanoma A375 cells

Authors:
- Taihen Yu
- Shi Zhong
- Yuqing Sun
- Haiyan Sun
- Weiguo Chen
- Yougui Li
- Jianxun Zhu
- Longxi Lu
- Jinxi Huo
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Affiliations: Department of Radiology, Zhejiang Provincial People's Hospital, Affiliated People's Hospital of Hangzhou Medical College, Hangzhou, Zhejiang 310014, P.R. China, Institute of Sericultural and Tea, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, P.R. China, Haining Economic Crop Technical Service Station, Haining Agricultural and Rural Bureau, Haining, Zhejiang 314400, P.R. China, Department of Environment & Health, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang 310051, P.R. China
Published online on: June 30, 2021 https://doi.org/10.3892/ol.2021.12889
Article Number: 628
Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Sanghuangporus vaninii, also called ‘Sanghuang’ mushroom in Chinese, has various medicinal uses, but its effects on human melanoma cells have not been reported. The present study investigated the inhibitory ability and potential anticancer mechanism of the aqueous extracts of S. vaninii (SH). The results revealed that SH inhibited the proliferation of A375 human melanoma cells in a dose‑dependent manner, and flow cytometry analysis suggested that SH induced A375 cell cycle arrest at S phase and apoptosis. Reverse transcription‑quantitative PCR, western blotting and immunofluorescence analyses indicated that SH induced S‑phase arrest by upregulating p21 expression, and p21 inhibited the expression of cyclin‑cyclin‑dependent kinases complexes at both the RNA and protein levels. In addition, SH induced apoptosis of A375 cells by inhibiting the expression levels of the anti‑apoptosis gene Bcl‑2. Therefore, the results suggested that SH may be a potential candidate for the treatment of human melanoma, thus providing new ideas for developing drugs that target melanoma.

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