search button
Open Access

miR‑148a, miR‑152 and miR‑200b promote prostate cancer metastasis by targeting DNMT1 and PTEN expression

  • Authors:
    • Venhar Gurbuz
    • Sinan Sozen
    • Cenk Y. Bilen
    • Ece Konac

  • View Affiliations

  • Published online on: September 23, 2021     https://doi.org/10.3892/ol.2021.13066
  • Article Number: 805

  • Copyright: © Gurbuz et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

MicroRNAs (miRs) modulate the expression of target genes in the signal pathway on transcriptome level. The present study investigated the ‘epigenetic‑based miRNA (epi‑miRNA)‑mRNA’ regulatory network of miR‑34b, miR‑34c, miR‑148a, miR‑152, miR‑200a and miR‑200b epi‑miRNAs and their target genes, DNA methyltransferase (DNMT1, 3a and 3b), phosphate and tensin homolog (PTEN) and NK3 Homeobox 1 (NKX3.1), in prostate cancer (PCa) using reverse transcription‑quantitative PCR. The expression level of NKX3.1 were not significantly different between the PCa, Met‑PCa and control groups. However, in the PCa and Met‑PCa groups, the expression level of DNMT1 was upregulated, while DNMT3a, DNMT3b and PTEN were downregulated. Overexpression of DNMT1 (~5 and ~6‑fold increase in the PCa and Met‑PCa groups respectively) was accompanied by a decreased expression in PTEN, indicating a potential negative association. Both groups indicated that a high level of DNMT1 is associated with the aggressiveness of cancer, and there is a a directly proportional relationship between this gene and PSA, GS and TNM staging. A significant ~2 to ~5‑fold decrease in the expression levels of DNMT3a and DNMT3b was found in both groups. In the PCa group, significant associations were identified between miR‑34b and DNMT1/DNMT3b; between miR‑34c/miR‑148a and all target genes; between miR‑152 and DNMT1/DNMT3b and PTEN; and between miR‑200a/b and DNMT1. In the Met‑PCa group, miR‑148a, miR‑152 and miR‑200b exhibited a significant association with all target genes. A significant negative association was identified between PTEN and DNMT1 in the Met‑PCa group. It was also revealed that that miR‑148a, miR‑152 and miR‑200b increased the expression of DNMT1 and suppressed PTEN. Furthermore, the ‘epi‑miRNA‑mRNA’ bidirectional feedback loop was emphasised and the methylation pattern in PCa anti‑cancer therapeutics was highlighted.
View Figures
View References

Related Articles

Journal Cover

November-2021
Volume 22 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

  • Journal Metrics
  • Impact Factor: 2.5
  • Ranked Oncology
    (total number of cites)
  • CiteScore: 5.7
  • CiteScore Rank: Q2: #139/404 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.574
  • SCImago Journal Rank (SJR): 0.644
International Symposium on Molecular Medicine Conference Banner
The Cancer Story
World Congress on Advances in Oncology and International Symposium on Molecular Medicine Conference Banner
World Academy Of Sciences Banner
Heyday Road Banner

0

Copy and paste a formatted citation
x
Spandidos Publications style
Gurbuz V, Sozen S, Bilen CY and Konac E: miR‑148a, miR‑152 and miR‑200b promote prostate cancer metastasis by targeting DNMT1 and PTEN expression. Oncol Lett 22: 805, 2021.
APA
Gurbuz, V., Sozen, S., Bilen, C.Y., & Konac, E. (2021). miR‑148a, miR‑152 and miR‑200b promote prostate cancer metastasis by targeting DNMT1 and PTEN expression. Oncology Letters, 22, 805. https://doi.org/10.3892/ol.2021.13066
MLA
Gurbuz, V., Sozen, S., Bilen, C. Y., Konac, E."miR‑148a, miR‑152 and miR‑200b promote prostate cancer metastasis by targeting DNMT1 and PTEN expression". Oncology Letters 22.5 (2021): 805.
Chicago
Gurbuz, V., Sozen, S., Bilen, C. Y., Konac, E."miR‑148a, miR‑152 and miR‑200b promote prostate cancer metastasis by targeting DNMT1 and PTEN expression". Oncology Letters 22, no. 5 (2021): 805. https://doi.org/10.3892/ol.2021.13066