Establishment and characterization of prostate organoids from treatment‑naïve patients with prostate cancer

Cheaito,Katia; Bahmad,Hisham F.; Hadadeh,Ola; Msheik,Hiba; Monzer,Alissar; Ballout,Farah; Dagher,Christelle; Telvizian,Talar; Saheb,Nour; Tawil,Ayman; El‑Sabban,Marwan; El‑Hajj,Albert; Mukherji,Deborah; Al‑Sayegh,Mohamed; Abou‑Kheir,Wassim

doi:10.3892/ol.2021.13124

Establishment and characterization of prostate organoids from treatment‑naïve patients with prostate cancer

Authors:
- Katia Cheaito
- Hisham F. Bahmad
- Ola Hadadeh
- Hiba Msheik
- Alissar Monzer
- Farah Ballout
- Christelle Dagher
- Talar Telvizian
- Nour Saheb
- Ayman Tawil
- Marwan El‑Sabban
- Albert El‑Hajj
- Deborah Mukherji
- Mohamed Al‑Sayegh
- Wassim Abou‑Kheir
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Affiliations: Department of Anatomy, Cell Biology and Physiological Sciences, Faculty of Medicine, American University of Beirut, Beirut 1107‑2020, Lebanon, Department of Internal Medicine, Division of Hematology/Oncology, Faculty of Medicine, American University of Beirut Medical Center, Beirut 1107‑2020, Lebanon, Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, Beirut 1107‑2020, Lebanon, Department of Surgery, Division of Urology, American University of Beirut Medical Center, Beirut 1107‑2020, Lebanon, Biology Division, New York University Abu Dhabi, Abu Dhabi, United Arab Emirates
Published online on: November 5, 2021 https://doi.org/10.3892/ol.2021.13124
Article Number: 6
Copyright: © Cheaito et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Three‑dimensional (3D) organoid culture systems are emerging as potential reliable tools to investigate basic developmental processes of human disease, especially cancer. The present study used established and modified culture conditions to report successful generation and characterization of patient‑derived organoids from fresh primary tissue specimens of patients with treatment‑naïve prostate cancer (PCa). Fresh tissue specimens were collected, digested enzymatically and the resulting cell suspensions were plated in a 3D environment using Matrigel as an extracellular matrix. Previously established 12‑factor medium for organoid culturing was modified to create a minimal 5‑factor medium. Organoids and corresponding tissue specimens were characterized using transcriptomic analysis, immunofluorescent analysis, and immunohistochemistry. Furthermore, patient‑derived organoids were used to assess the drug response. Treatment‑naïve patient‑derived PCa organoids were obtained from fresh radical prostatectomy specimens. These PCa organoids mimicked the heterogeneity of corresponding parental tumor tissue. Histopathological analysis demonstrated similar tissue architecture and cellular morphology, as well as consistent immunohistochemical marker expression. Also, the results confirmed the potential of organoids as an in vitro model to assess potential personalized treatment responses as there was a differential drug response between different patient samples. In conclusion, the present study investigated patient‑derived organoids from a cohort of treatment‑naïve patients. Derived organoids mimicked the histological features and prostate lineage profiles of their corresponding parental tissue and may present a potential model to predict patient‑specific treatment response in a pre‑clinical setting.

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