PI3K/Akt/YAP signaling promotes migration and invasion of DLD‑1 colorectal cancer cells

  • Authors:
    • Tomoya Takeda
    • Yuuta Yamamoto
    • Masanobu Tsubaki
    • Takuya Matsuda
    • Akihiro Kimura
    • Natsumi Shimo
    • Shozo Nishida
  • View Affiliations

  • Published online on: February 3, 2022     https://doi.org/10.3892/ol.2022.13226
  • Article Number: 106
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Abstract

Colorectal cancer (CRC) is one of the most prevalent malignant diseases and metastasis is the leading cause of poor prognosis in patients with CRC. Further knowledge of the molecular mechanism underlying metastasis in CRC and the identification of new therapeutic targets are needed. Yes‑associated protein (YAP) is a transcriptional regulator that is important in tumorigenesis and tumor cell proliferation. The present study investigated whether YAP was crucial for CRC migration and invasion. The protein expression levels were detected via western blotting, and migration and invasion were analyzed by Transwell migration and invasion assays. Subsequently, YAP expression was silenced using small interfering RNA. The mRNA expression levels were detected via reverse transcription‑quantitative PCR and cell viability was assessed via Trypan blue exclusion assay. The results revealed that YAP protein levels were associated with migration and invasion of CRC cells. Notably, YAP small interfering RNA inhibited the migration and invasion of DLD‑1 cells. In addition, the phosphoinositide 3‑kinase (PI3K)/Akt signaling pathway inhibitor LY294002 suppressed the migration and invasion of DLD‑1 cells by decreasing the expression of YAP. Notably, the present study demonstrated that verteporfin mediated the suppression of migration and invasion of DLD‑1 cells due to the decreased expression of YAP. Therefore, targeting YAP may be valuable for developing therapeutic strategies against CRC, and verteporfin may be an effective therapy to suppress the migration and invasion of CRC.
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April-2022
Volume 23 Issue 4

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Spandidos Publications style
Takeda T, Yamamoto Y, Tsubaki M, Matsuda T, Kimura A, Shimo N and Nishida S: PI3K/Akt/YAP signaling promotes migration and invasion of DLD‑1 colorectal cancer cells. Oncol Lett 23: 106, 2022.
APA
Takeda, T., Yamamoto, Y., Tsubaki, M., Matsuda, T., Kimura, A., Shimo, N., & Nishida, S. (2022). PI3K/Akt/YAP signaling promotes migration and invasion of DLD‑1 colorectal cancer cells. Oncology Letters, 23, 106. https://doi.org/10.3892/ol.2022.13226
MLA
Takeda, T., Yamamoto, Y., Tsubaki, M., Matsuda, T., Kimura, A., Shimo, N., Nishida, S."PI3K/Akt/YAP signaling promotes migration and invasion of DLD‑1 colorectal cancer cells". Oncology Letters 23.4 (2022): 106.
Chicago
Takeda, T., Yamamoto, Y., Tsubaki, M., Matsuda, T., Kimura, A., Shimo, N., Nishida, S."PI3K/Akt/YAP signaling promotes migration and invasion of DLD‑1 colorectal cancer cells". Oncology Letters 23, no. 4 (2022): 106. https://doi.org/10.3892/ol.2022.13226