TPX2 regulated by miR‑29c‑3p induces cell proliferation in osteosarcoma via the AKT signaling pathway

Zhu,Dongsheng; Xu,Xiangfei; Zhang,Ming; Wang,Tong

doi:10.3892/ol.2022.13262

TPX2 regulated by miR‑29c‑3p induces cell proliferation in osteosarcoma via the AKT signaling pathway

Authors:
- Dongsheng Zhu
- Xiangfei Xu
- Ming Zhang
- Tong Wang
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Affiliations: Department of Pediatric Surgery, The First People's Hospital of Lianyungang Affiliated to Xuzhou Medical University, Lianyungang, Jiangsu 222000, P.R. China
Published online on: March 11, 2022 https://doi.org/10.3892/ol.2022.13262
Article Number: 143
Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to investigate the significance of targeting protein for Xenopus kinesin‑like protein 2 (TPX2) expression in osteosarcoma. First, the TPX2 expression and survival analysis data were evaluated from The Cancer Genome Atlas (TCGA) database. Next, reverse transcription‑quantitative PCR was used to explore the expression of TPX2 in osteosarcoma tissues. The observed potential target relationship between TPX2 and microRNA (miR)‑29c‑3p was verified using TargetScan and luciferase reporter assays. Kaplan‑Meier survival analysis was used to determine associations between TPX2 expression levels and survival prognosis. TPX2 small interfering RNA was successfully constructed and transfected into osteosarcoma cell lines. The effects of TPX2 on osteosarcoma cell proliferation were then detected by MTT assay. In addition, the expression levels of AKT signaling pathway‑associated proteins were identified by western blot analysis. The expression of TPX2 was upregulated and the expression of miR‑29c‑3p was downregulated in osteosarcoma. High expression of TPX2 was linked to a poor prognosis. Using luciferase assay and the miRNA mimic and inhibitors, miR‑29c‑3p was able to target and repress TPX2, and siRNA knockdown of TPX2 resulted in the inhibition of osteosarcoma cell proliferation by affecting the AKT pathway. Overall, the study showed that miR‑29c‑3p could inhibit the proliferation of osteosarcoma cells via TPX2 downregulation, and that TPX2 and miR‑29c‑3p may serve as promising prognostic indicators.

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