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Comparative analysis of transient receptor potential channel 5 opposite strand‑induced gene expression patterns and protein‑protein interactions in triple‑negative breast cancer

  • Authors:
    • Jinghui Peng
    • Shengbin Pei
    • Yangyang Cui
    • Yiqin Xia
    • Yue Huang
    • Xiaowei Wu
    • Mingjie Zheng
    • Miaomiao Weng
    • Xu Han
    • Hongtao Fu
    • Lili Yang
    • Wenbin Zhou
    • Ziyi Fu
    • Shui Wang
    • Hui Xie

  • View Affiliations

  • Published online on: June 14, 2022     https://doi.org/10.3892/ol.2022.13379
  • Article Number: 259

  • Copyright: © Peng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

In patients with triple‑negative breast cancer (TNBC), high tumour mutation burden and aberrant oncogene expression profiles are some of the causes of poor prognosis. Therefore, it is necessary to identify aberrantly expressed oncogenes, since they have the potential to serve as therapeutic targets. Transient receptor potential channel 5 opposite strand (TRPC5OS) has been previously shown to function as a novel tumour inducer. However, the underlying mechanism of TRPC5OS function in TNBC remain to be elucidated. Therefore, in the present study TRPC5OS expression was first measured in tissue samples of patients with TNBC and a panel of breast cancer cell lines (ZR‑75‑1, MDA‑MB‑453, SK‑BR‑3, JIMT‑1, BT474 and HCC1937) by using qRT‑PCR and Western blotting. Subsequently, the possible effects of TRPC5OS on MDA‑MB‑231 cells proliferation were determined using Cell Counting Kit‑8 and 5‑Ethynyl‑2'‑deoxyuridine assays after Lentiviral transfection of MDA‑MB‑231. In addition, potential interaction partners of TRPC5OS were explored using liquid chromatography‑mass spectrometry (LC‑MS)/MS. Gene expression patterns following TRPC5OS overexpression were also detected in MDA‑MB‑231 cells by using High‑throughput sequencing. Gene Ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) analysis were then used to systematically verify the potential interactions among the TRPC5OS‑regulated genes. The potential relationship between TRPC5OS‑interacting proteins and gene expression patterns were studied using Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) analysis. TRPC5OS expression was found to be significantly higher in TNBC tumour tissues and breast cancer cell lines compared with luminal tumour tissues and ZR‑75‑1. In addition, the overexpression of TRPC5OS significantly increased cell proliferation. High‑throughput sequencing results revealed that 5,256 genes exhibited differential expression following TRPC5OS overexpression, including 3,269 upregulated genes and 1,987 downregulated genes. GO analysis results indicated that the functions of these differentially expressed genes were enriched in the categories of ‘cell division’ and ‘cell proliferation’ regulation. KEGG analysis showed that the TRPC5OS‑regulated genes were associated with processes of ‘homologous recombination’ and ‘TNF signalling pathways’. Subsequently, 17 TRPC5OS‑interacting proteins were found using LC‑MS/MS and STRING analysis. The most important protein among interacting proteins was ENO1 which was associated with glycolysis and regulated proliferation of cancer. In summary, data from the present study suggest that TRPC5OS overexpression can increase TNBC cell proliferation and ENO1 may be a potential target protein mediated by TRPC5OS. Therefore, TRPC5OS may serve as a novel therapeutic target for TNBC.
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August-2022
Volume 24 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Peng J, Pei S, Cui Y, Xia Y, Huang Y, Wu X, Zheng M, Weng M, Han X, Fu H, Fu H, et al: Comparative analysis of transient receptor potential channel 5 opposite strand‑induced gene expression patterns and protein‑protein interactions in triple‑negative breast cancer. Oncol Lett 24: 259, 2022
APA
Peng, J., Pei, S., Cui, Y., Xia, Y., Huang, Y., Wu, X. ... Xie, H. (2022). Comparative analysis of transient receptor potential channel 5 opposite strand‑induced gene expression patterns and protein‑protein interactions in triple‑negative breast cancer. Oncology Letters, 24, 259. https://doi.org/10.3892/ol.2022.13379
MLA
Peng, J., Pei, S., Cui, Y., Xia, Y., Huang, Y., Wu, X., Zheng, M., Weng, M., Han, X., Fu, H., Yang, L., Zhou, W., Fu, Z., Wang, S., Xie, H."Comparative analysis of transient receptor potential channel 5 opposite strand‑induced gene expression patterns and protein‑protein interactions in triple‑negative breast cancer". Oncology Letters 24.2 (2022): 259.
Chicago
Peng, J., Pei, S., Cui, Y., Xia, Y., Huang, Y., Wu, X., Zheng, M., Weng, M., Han, X., Fu, H., Yang, L., Zhou, W., Fu, Z., Wang, S., Xie, H."Comparative analysis of transient receptor potential channel 5 opposite strand‑induced gene expression patterns and protein‑protein interactions in triple‑negative breast cancer". Oncology Letters 24, no. 2 (2022): 259. https://doi.org/10.3892/ol.2022.13379