Open Access

Differentially expressed genes related to lymph node metastasis in advanced laryngeal squamous cell cancers

  • Authors:
    • Ömer Bayır
    • Mehmet Doğan Aşık
    • Güleser Saylam
    • Ferda Alpaslan Pınarlı
    • Emel Çadallı Tatar
    • Ünsal Han
    • Ender Şi̇mşek
    • Mehmet Hakan Korkmaz
  • View Affiliations

  • Published online on: September 26, 2022     https://doi.org/10.3892/ol.2022.13529
  • Article Number: 409
  • Copyright: © Bayır et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Understanding the molecular mechanisms and gene expression in laryngeal squamous cell carcinoma (LSCC) may explain its aggressive biological behavior and regional metastasis pathways. In the present study, patients with locally advanced LSCC tumors were examined for differential gene expression in the normal mucosa (non-tumoral mucosa), tumors and lymph node tissues. The aim was to identify possible predictive genes for lymph node metastasis. A total of 16 patients who had undergone total laryngectomy with neck dissection for advanced LSCC were randomly selected from a hospital database: Eight of the patients had lymph node metastasis (Group 1) and the other eight patients did not have metastasis (Group 2). Overall survival (OS), disease‑free survival (DFS) and disease‑specific survival (DSS) were analyzed. For each patient, paraffin‑embedded tissue samples were collected from non‑tumoral mucosa, tumoral lesions and lymph node tissues. RNA was extracted from the tissue samples and used for complementary DNA synthesis, and microarray analysis was subsequently performed on each sample. Gene expression levels were determined in each specimen, and Groups 1 and 2 were compared and statistically analyzed. The microarray results for lymph node metastasis‑positive and ‑negative groups, indicated the differential expression of 312 genes in the lymph nodes, 691 genes in the normal mucosal tissue and 93 genes in the tumor tissue. Transgelin (TAGLN) and cofilin 1 (CFL1) were identified as possible target genes and validated using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). The RT‑qPCR results for TAGLN and CFL1 supported the microarray data. OS, DFS and DSS times were longer in Group 2 than in Group 1 (P=0.002, 0.015 and 0.009, respectively). In addition, TAGLN and CFL1 were associated with DFS and DSS. On the basis of these results, it is suggested that TAGLN and CFL1 expression may play an important role in the pathogenesis of regional metastasis and poor prognosis in advanced LSCC.
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November-2022
Volume 24 Issue 5

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Spandidos Publications style
Bayır Ö, Aşık MD, Saylam G, Pınarlı FA, Tatar EÇ, Han Ü, Şi̇mşek E and Korkmaz MH: Differentially expressed genes related to lymph node metastasis in advanced laryngeal squamous cell cancers. Oncol Lett 24: 409, 2022.
APA
Bayır, Ö., Aşık, M.D., Saylam, G., Pınarlı, F.A., Tatar, E.Ç., Han, Ü. ... Korkmaz, M.H. (2022). Differentially expressed genes related to lymph node metastasis in advanced laryngeal squamous cell cancers. Oncology Letters, 24, 409. https://doi.org/10.3892/ol.2022.13529
MLA
Bayır, Ö., Aşık, M. D., Saylam, G., Pınarlı, F. A., Tatar, E. Ç., Han, Ü., Şi̇mşek, E., Korkmaz, M. H."Differentially expressed genes related to lymph node metastasis in advanced laryngeal squamous cell cancers". Oncology Letters 24.5 (2022): 409.
Chicago
Bayır, Ö., Aşık, M. D., Saylam, G., Pınarlı, F. A., Tatar, E. Ç., Han, Ü., Şi̇mşek, E., Korkmaz, M. H."Differentially expressed genes related to lymph node metastasis in advanced laryngeal squamous cell cancers". Oncology Letters 24, no. 5 (2022): 409. https://doi.org/10.3892/ol.2022.13529