Macamide B suppresses lung cancer progression potentially via the ATM signaling pathway

Tao,Haiyan; Shi,Hubo; Wang,Min; Xu,Yihui

doi:10.3892/ol.2023.13701

Macamide B suppresses lung cancer progression potentially via the ATM signaling pathway

Authors:
- Haiyan Tao
- Hubo Shi
- Min Wang
- Yihui Xu
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Affiliations: Department of Acupuncture and Massage, Central Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250013, P.R. China, Department of Thoracic Surgery, Shandong Public Health Clinical Center, Jinan, Shandong 250102, P.R. China, Medical Research and Laboratory Diagnostic Center, Central Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250013, P.R. China
Published online on: February 6, 2023 https://doi.org/10.3892/ol.2023.13701
Article Number: 115
Copyright: © Tao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Macamides are a class of bioactive natural products obtained from Lepidium meyenii (maca), which have been reported to exert inhibitory activity in cancer. However, their role in lung cancer is currently unknown. In the present study, macamide B was shown to inhibit the proliferation and invasion of lung cancer cells, as determined by Cell Counting Kit‑8 and Transwell assays, respectively. By contrast, macamide B induced cell apoptosis, as determined by Annexin V‑FITC assay. Moreover, combined treatment with macamide B and olaparib, an inhibitor of poly (ADP‑ribose) polymerase, further suppressed the proliferation of lung cancer cells. At the molecular level, the expression of ataxia‑telangiectasia mutated (ATM), RAD51, p53 and cleaved caspase‑3 were significantly increased by macamide B, as determined by western blotting, whereas the expression levels of Bcl‑2 were decreased. By contrast, when ATM expression was knocked down by small interfering RNA technology in A549 cells treated with macamide B, the expression levels of ATM, RAD51, p53 and cleaved caspase‑3 were reduced, whereas those of Bcl‑2 were increased. Consistently, cell proliferation and invasive ability were partially rescued by ATM knockdown. In conclusion, macamide B inhibits lung cancer progression by inhibiting cell proliferation and invasion, and inducing apoptosis. Furthermore, macamide B may participate in regulating the ATM signaling pathway. The present study provides a potential new natural drug for treating patients with lung cancer.

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