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Lanosterol synthase loss of function decreases the malignant phenotypes of HepG2 cells by deactivating the Src/MAPK signaling pathway

  • Authors:
    • Xiaomei Sun
    • Jun Zhang
    • Hui Liu
    • Mingcong Li
    • Li Liu
    • Zhen Yang
    • Weikang Hu
    • Hongmei Bai
    • Jiansheng Xu
    • Jun Xing
    • Zhijun Xu
    • Aizhu Mo
    • Ziyi Guo
    • Yajie  Bai
    • Qing Zhou
    • Yuan Wang
    • Shengquan Zhang
    • Sumei Zhang

  • View Affiliations

  • Published online on: May 23, 2023     https://doi.org/10.3892/ol.2023.13881
  • Article Number: 295

  • Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cholesterol is critical for tumor cells to maintain their membrane components, cell morphology and activity functions. The inhibition of the cholesterol pathway may be an efficient strategy with which to limit tumor growth and the metastatic process. In the present study, lanosterol synthase (LSS) was knocked down by transfecting LSS short hairpin RNA into HepG2 cells, and cell growth, apoptosis and migratory potential were then detected by Cell Counting Kit‑8 cell proliferation assay, flow cytometric analysis and wound healing assay, respectively. In addition, proteins associated with the regulation of the aforementioned cell biological behaviors were analyzed by western blot analysis. The activity of the Src/MAPK signaling pathway was measured by western blotting to elucidate the possible signal transduction mechanisms. LSS knockdown in the HepG2 liver cancer cell line inhibited cell proliferation, with cell cycle arrest at the S phase; it also decreased cell migratory ability and increased apoptosis. The expression proteins involved in the regulation of cell cycle, cell apoptosis and migration was altered by LSS knockdown in HepG2 cells. Furthermore, a decreased Src/MAPK activity was observed in the HepG2 cells subjected to LSS knockdown. LSS loss of function decreased the malignant phenotypes of HepG2 cells by deactivating the Src/MAPK signaling pathway and regulating expression of genes involved in cell cycle regulation, cell apoptosis and migration.
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July-2023
Volume 26 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Sun X, Zhang J, Liu H, Li M, Liu L, Yang Z, Hu W, Bai H, Xu J, Xing J, Xing J, et al: Lanosterol synthase loss of function decreases the malignant phenotypes of HepG2 cells by deactivating the Src/MAPK signaling pathway. Oncol Lett 26: 295, 2023.
APA
Sun, X., Zhang, J., Liu, H., Li, M., Liu, L., Yang, Z. ... Zhang, S. (2023). Lanosterol synthase loss of function decreases the malignant phenotypes of HepG2 cells by deactivating the Src/MAPK signaling pathway. Oncology Letters, 26, 295. https://doi.org/10.3892/ol.2023.13881
MLA
Sun, X., Zhang, J., Liu, H., Li, M., Liu, L., Yang, Z., Hu, W., Bai, H., Xu, J., Xing, J., Xu, Z., Mo, A., Guo, Z., Bai, Y., Zhou, Q., Wang, Y., Zhang, S., Zhang, S."Lanosterol synthase loss of function decreases the malignant phenotypes of HepG2 cells by deactivating the Src/MAPK signaling pathway". Oncology Letters 26.1 (2023): 295.
Chicago
Sun, X., Zhang, J., Liu, H., Li, M., Liu, L., Yang, Z., Hu, W., Bai, H., Xu, J., Xing, J., Xu, Z., Mo, A., Guo, Z., Bai, Y., Zhou, Q., Wang, Y., Zhang, S., Zhang, S."Lanosterol synthase loss of function decreases the malignant phenotypes of HepG2 cells by deactivating the Src/MAPK signaling pathway". Oncology Letters 26, no. 1 (2023): 295. https://doi.org/10.3892/ol.2023.13881