Upregulation of ubiquitin carboxy‑terminal
hydrolase 47 (USP47) in papillary thyroid carcinoma ex vivo and
reduction of tumor cell malignant behaviors after USP47 knockdown by stabilizing SATB1 expression in vitro

  • Authors:
    • Guirong Luo
    • Liting Zhang
    • Wenyi Wu
    • Lihong Zhang
    • Jianqing Lin
    • Haihong Shi
    • Xinquan Wu
    • Yihuang Yu
    • Weigang Qiu
    • Jinyan Chen
    • Hansen Ding
    • Xinyao Chen
  • View Affiliations

  • Published online on: July 17, 2023     https://doi.org/10.3892/ol.2023.13956
  • Article Number: 370
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Abstract

Aberrant ubiquitination contributes to cancer development, including thyroid carcinoma. The present study assessed the expression of ubiquitin carboxy‑terminal hydrolase 47 (USP47) and underlying molecular events in the development of papillary thyroid carcinoma (PTC). The effects of USP47 on PTC cell invasion and migration were analyzed by Transwell assays, while. the effects of USP47 and SATB1on PTC cell gene expression and changes in tumor cell metabolism were assayed by reverse transcription‑quantitative PCR, western bolt, or ELISA, respectively. The expression of USP47 mRNA and protein was upregulated in PTC tissue and associated with the PTC tumor size. Knockdown of USP47 expression in PTC cell lines (TPC‑1 and K1), decreased the cell proliferation mobility and invasion capacities, whereas USP47 overexpression in these cell lines showed an inverse effect and promoted cell glycolysis and glutamine metabolism. Moreover, expression of special AT‑rich sequence‑binding protein‑1 (SATB1) was high in PTC tissue and was associated with USP47 expression. SATB1 expression promoted tumor cell glycolysis and glutamine metabolism, while USP47 protein bound to and deubiquitinated SATB1 to increase its intracellular levels, thus promoting glycolysis and glutamine metabolism. USP47 promotion of PTC development may be due to its stabilization of SATB1 protein, suggesting that targeting the USP47/SATB1 signaling axis may serve as a therapeutic intervention for PTC.
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September-2023
Volume 26 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Luo G, Zhang L, Wu W, Zhang L, Lin J, Shi H, Wu X, Yu Y, Qiu W, Chen J, Chen J, et al: Upregulation of ubiquitin carboxy‑terminal <br />hydrolase 47 (USP47) in papillary thyroid carcinoma ex vivo and <br />reduction of tumor cell malignant behaviors after USP47 knockdown by stabilizing SATB1 expression in vitro. Oncol Lett 26: 370, 2023.
APA
Luo, G., Zhang, L., Wu, W., Zhang, L., Lin, J., Shi, H. ... Chen, X. (2023). Upregulation of ubiquitin carboxy‑terminal <br />hydrolase 47 (USP47) in papillary thyroid carcinoma ex vivo and <br />reduction of tumor cell malignant behaviors after USP47 knockdown by stabilizing SATB1 expression in vitro. Oncology Letters, 26, 370. https://doi.org/10.3892/ol.2023.13956
MLA
Luo, G., Zhang, L., Wu, W., Zhang, L., Lin, J., Shi, H., Wu, X., Yu, Y., Qiu, W., Chen, J., Ding, H., Chen, X."Upregulation of ubiquitin carboxy‑terminal <br />hydrolase 47 (USP47) in papillary thyroid carcinoma ex vivo and <br />reduction of tumor cell malignant behaviors after USP47 knockdown by stabilizing SATB1 expression in vitro". Oncology Letters 26.3 (2023): 370.
Chicago
Luo, G., Zhang, L., Wu, W., Zhang, L., Lin, J., Shi, H., Wu, X., Yu, Y., Qiu, W., Chen, J., Ding, H., Chen, X."Upregulation of ubiquitin carboxy‑terminal <br />hydrolase 47 (USP47) in papillary thyroid carcinoma ex vivo and <br />reduction of tumor cell malignant behaviors after USP47 knockdown by stabilizing SATB1 expression in vitro". Oncology Letters 26, no. 3 (2023): 370. https://doi.org/10.3892/ol.2023.13956