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Upregulated miR‑378a‑3p expression suppresses energy metabolism and promotes apoptosis by targeting a GLUT‑1/ALDOA/PKM2 axis in esophageal carcinoma

  • Authors:
    • Yuan Qu
    • Shan Xue
    • Yujian Zheng
    • Yajing Du
    • Guoping Zhang
    • Liting Huang
    • Hui Li
    • Huiwu Li

  • View Affiliations

  • Published online on: August 10, 2023     https://doi.org/10.3892/ol.2023.14007
  • Article Number: 421

  • Copyright: © Qu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Esophageal squamous cell carcinoma (ESCC) is an aggressive malignancy of the digestive system with increasing incidence and mortality rates. The biological roles of microRNA (miR)‑378a‑3p in tumor cells remain contested, and the mechanisms underlying the functions, energy metabolism, and cell survival mechanisms in ESCC cells are yet to be fully elucidated. In the present study, miR‑378a‑3p overexpression and negative control plasmids were transfected into ECA‑109 cells using electroporation. Western blotting was used to detect the relative expression of proteins, and flow cytometry was used to detect cell apoptosis. Subsequently, ELISA assays were performed to determine enzyme activity, and an ATP detection kit was used to measure ATP content. Dual‑luciferase reporter assays were performed to identify the target genes of miR‑378a‑3p. The results of the present study demonstrated that miR‑378a‑3p inhibited the gene expression and enzyme activities of glucose transporter protein 1 (GLUT‑1), Aldolase A (ALDOA), and pyruvate kinase M2 (PKM2), all of which are involved in the glycolytic pathway of cells. Energy metabolism was suppressed by miR‑378a‑3p by reducing ATP content, and this downregulated the expression of Bcl‑2 and Survivin. Moreover, increased miR‑378a‑3p expression promoted cell apoptosis in the early stages by increasing the expression levels and the activity of Bad and Caspase‑3, while inhibiting the expression levels of Bcl‑2 and Survivin. The results of the present study also demonstrated that GLUT‑1/ALDOA/PKM2 were target genes of miR‑378a‑3p. Notably, miR‑378a‑3p blocked energy production and promoted the apoptosis of tumor cells via the downregulation of glycolytic enzyme expression and by reducing the mitochondrial membrane potential in ESCC. Bad, Caspase‑3, Survivin, and Bcl‑2 may be associated with blocking energy production and promoting apoptosis via miR‑378a‑3p in ESCC cells.
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October-2023
Volume 26 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Qu Y, Xue S, Zheng Y, Du Y, Zhang G, Huang L, Li H and Li H: Upregulated miR‑378a‑3p expression suppresses energy metabolism and promotes apoptosis by targeting a GLUT‑1/ALDOA/PKM2 axis in esophageal carcinoma. Oncol Lett 26: 421, 2023.
APA
Qu, Y., Xue, S., Zheng, Y., Du, Y., Zhang, G., Huang, L. ... Li, H. (2023). Upregulated miR‑378a‑3p expression suppresses energy metabolism and promotes apoptosis by targeting a GLUT‑1/ALDOA/PKM2 axis in esophageal carcinoma. Oncology Letters, 26, 421. https://doi.org/10.3892/ol.2023.14007
MLA
Qu, Y., Xue, S., Zheng, Y., Du, Y., Zhang, G., Huang, L., Li, H., Li, H."Upregulated miR‑378a‑3p expression suppresses energy metabolism and promotes apoptosis by targeting a GLUT‑1/ALDOA/PKM2 axis in esophageal carcinoma". Oncology Letters 26.4 (2023): 421.
Chicago
Qu, Y., Xue, S., Zheng, Y., Du, Y., Zhang, G., Huang, L., Li, H., Li, H."Upregulated miR‑378a‑3p expression suppresses energy metabolism and promotes apoptosis by targeting a GLUT‑1/ALDOA/PKM2 axis in esophageal carcinoma". Oncology Letters 26, no. 4 (2023): 421. https://doi.org/10.3892/ol.2023.14007