LRP1 as a promising therapeutic target for gastrointestinal tumors: Inhibiting proliferation, invasion and migration of cancer cells

Zhu,Mengying; Shen,Hao; Wang,Bili; He,Yingfei; Chen,Jin; Ren,Jun; Zhang,Zhezhong; Jian,Xu

doi:10.3892/ol.2023.14019

LRP1 as a promising therapeutic target for gastrointestinal tumors: Inhibiting proliferation, invasion and migration of cancer cells

Authors:
- Mengying Zhu
- Hao Shen
- Bili Wang
- Yingfei He
- Jin Chen
- Jun Ren
- Zhezhong Zhang
- Xu Jian
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Affiliations: School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, P.R. China, Lab Center, The Children's Hospital Zhejiang University School of Medicine, Hangzhou, Zhejiang 310052, P.R. China, Lab Center, The Children's Hospital Zhejiang University School of Medicine, Hangzhou, Zhejiang 310052, P.R. China, Department of Pathology, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, P.R. China, School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, P.R. China, Department of Clinical Laboratory, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310006, P.R. China
Published online on: August 22, 2023 https://doi.org/10.3892/ol.2023.14019
Article Number: 432
Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Gastrointestinal (GI) cancers are the most common types of tumors worldwide. The lack of cancer biomarkers and targeted drug resistance are barriers to achieving effective cancer therapy. Low‑density lipoprotein receptor‑related protein 1 (LRP1) is a transmembrane protein that has multiple functions due to its ability to recognize different ligands; however, the role of LRP1 in GI cancer cells remains unclear. The present study aimed to investigate the role of LRP1 in GI tumors. The Cancer Genome Atlas database was used to analyze the potential correlation between expression of LRP1 and prognosis in patients with GI cancer. Bioinformatics analysis was utilized and the expression of LRP1 was simultaneously validated in GI cancer at the cellular level through western blot experiments. LRP1 was expressed at high levels in HGC‑27, HepG2 and BxPC‑3 cells. LRP1 expression in GI cancer cells was knocked down using lentivirus‑mediated shRNA and the effects on biological functions were observed. LRP1 knockdown suppressed the proliferation, invasion and migration of GI cancer cells. LRP1 knockdown inhibited CD36 gene expression in HepG2 and BxPC‑3 cells. LRP1 knockdown inhibited the proliferation, invasion and migration of GI cancer cells, suggesting that LRP1 may be a novel target for treatment of GI tumors.

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