Increased abundance of <em>Firmicutes</em> and depletion of <em>Bacteroidota</em> predicts poor outcome in chronic lymphocytic leukemia

Paziewska,Magdalena; Szelest,Monika; Kiełbus,Michał; Masternak,Marta; Zaleska,Joanna; Wawrzyniak,Ewa; Kotkowska,Aleksandra; Siemieniuk-Ryś,Monika; Morawska,Marta; Kalicińska,Elżbieta; Jabłonowska,Paula; Wróbel,Tomasz; Wolska-Washer,Anna; Błoński,Jerzy Zdzisław; Robak,Tadeusz; Bullinger,Lars; Giannopoulos,Krzysztof

doi:10.3892/ol.2024.14685

Increased abundance of Firmicutes and depletion of Bacteroidota predicts poor outcome in chronic lymphocytic leukemia

Authors:
- Magdalena Paziewska
- Monika Szelest
- Michał Kiełbus
- Marta Masternak
- Joanna Zaleska
- Ewa Wawrzyniak
- Aleksandra Kotkowska
- Monika Siemieniuk-Ryś
- Marta Morawska
- Elżbieta Kalicińska
- Paula Jabłonowska
- Tomasz Wróbel
- Anna Wolska-Washer
- Jerzy Zdzisław Błoński
- Tadeusz Robak
- Lars Bullinger
- Krzysztof Giannopoulos
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Affiliations: Department of Experimental Hematooncology, Medical University of Lublin, 20-093 Lublin, Poland, Department of Hematology, Medical University of Lodz, 93-510 Lodz, Poland, Department of Hematology, Blood Neoplasms and Bone Marrow Transplantation, Wroclaw Medical University, 50-367 Wroclaw, Poland, Department of Experimental Hematology, Medical University of Lodz, 93-510 Lodz, Poland, Department of Hematology, Oncology and Cancer Immunology, Charité-Universitätsmedizin Berlin (Corporate Member of Free University of Berlin, Humboldt University of Berlin), D-13353 Berlin, Germany
Published online on: September 17, 2024 https://doi.org/10.3892/ol.2024.14685
Article Number: 552
Copyright : © Paziewska et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].

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Abstract

Evidence indicates that there are significant alterations in gut microbiota diversity and composition in patients with hematological malignancies. The present study investigated the oral and intestinal microbiome in patients with chronic lymphocytic leukemia (CLL) (n=81) and age‑matched healthy volunteers (HVs; n=21) using 16S ribosomal RNA next‑generation sequencing. Changes in both oral and gut microbiome structures were identified, with a high abundance of Proteobacteria and depletion of Bacteroidetes in CLL as compared to HVs. Oral and stool samples of patients with CLL revealed a significant change in the abundance of short‑chain fatty acid‑producing genera in comparison with HVs. Furthermore, the relative abundance of oral and intestine Bacteroidetes was significantly decreased in patients with CLL with negative prognostic features, including unmutated immunoglobulin heavy chain gene (IGHV). Notably, an increased abundance of gut Firmicutes was found to be associated with high expression of CD38. Finally, the present study suggested the log Firmicutes/Bacteroidota ratio as a novel intestinal microbiome signature associated with a shorter time to first treatment in individuals with CLL. The findings indicate that oral and gut microbial diversity in CLL might point to the inflammatory‑related modulation of the clinical course of the disease.

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