Suppression of TPA-induced tumor cell invasion by sulfuretin via inhibition of NF-κB-dependent MMP-9 expression

Kim,Jeong-Mi; Noh,Eun-Mi; Kwon,Kang-Beom; Kim,Jong-Suk; You,Yong-Ouk; Hwang,Jin-Ki; Hwang,Bo-Mi; Kim,Min Seuk; Lee,Seung-Jin; Jung,Sung-Hoo; Youn,Hyun-Jo; Chung,Eun-Yong; Lee,Young-Rae

doi:10.3892/or.2012.2218

Suppression of TPA-induced tumor cell invasion by sulfuretin via inhibition of NF-κB-dependent MMP-9 expression

Authors:
- Jeong-Mi Kim
- Eun-Mi Noh
- Kang-Beom Kwon
- Jong-Suk Kim
- Yong-Ouk You
- Jin-Ki Hwang
- Bo-Mi Hwang
- Min Seuk Kim
- Seung-Jin Lee
- Sung-Hoo Jung
- Hyun-Jo Youn
- Eun-Yong Chung
- Young-Rae Lee
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Affiliations: Center for Metabolic Function Regulation, Wonkwang University School of Medicine, Iksan 570-749, Republic of Korea, Department of Biochemistry, Institute of Medical Science, Chonbuk National University Medical School, Jeonju 560-182, Republic of Korea, Department of Oral Biochemistry, School of Dentistry, Wonkwang University, Iksan 570-749, Republic of Korea, Laboratory of Oral Physiology, College of Dentistry, Wonkwang University, Iksan 570-749, Republic of Korea, Department of Pharmacy, College of Pharmacy, Ewha Womans University, Seoul 120-808, Republic of Korea, Division of Breast, Thyroid Surgery, Department of Surgery, Chonbuk National University Medical School, Jeonju 560-756, Republic of Korea, Department of Anesthesiology and Pain Medicine, Bucheon St. Mary's Hospital, The Catholic University of Korea, Bucheon 420-717, Republic of Korea
Published online on: December 28, 2012 https://doi.org/10.3892/or.2012.2218
Pages: 1231-1237

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Abstract

Cell invasion is required for neoplastic metastasis. Matrix metalloproteinase-9 (MMP-9), which degrades the extracellular matrix, is a major component in the process of cancer cell invasion. Sulfuretin is one of the major flavonoids isolated from Rhus verniciflua. Sulfuretin has been used to reduce oxidative stress, platelet aggregation, the inflammatory response and mutagenesis. However, the effect of sulfuretin on breast cancer metastasis is unknown. In this study, we investigated the inhibitory effect of sulfuretin on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced MMP-9 expression and cell invasion in MCF-7 cells. Sulfuretin inhibited TPA-induced transcriptional activation of nuclear factor-κB (NF-κB). We demonstrated that sulfuretin mediated the inhibition of TPA-induced MMP-9 expression and that cell invasion in MCF-7 cells involved suppression of the NF-κB pathway. Therefore, inhibiting MMP-9 expression by sulfuretin may have therapeutic potential for controlling breast cancer invasiveness.

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