The saponin monomer of dwarf lilyturf tuber, DT-13, inhibits angiogenesis under hypoxia and normoxia via multi-targeting activity

Zhao,Renping; Sun,Li; Lin,Sensen; Bai,Xianshu; Yu,Boyang; Yuan,Shengtao; Zhang,Luyong

doi:10.3892/or.2013.2272

The saponin monomer of dwarf lilyturf tuber, DT-13, inhibits angiogenesis under hypoxia and normoxia via multi-targeting activity

Authors:
- Renping Zhao
- Li Sun
- Sensen Lin
- Xianshu Bai
- Boyang Yu
- Shengtao Yuan
- Luyong Zhang
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Affiliations: Jiangsu Center for Pharmacodynamics Research and Evaluation, China Pharmaceutical University, Nanjing, Jiangsu 210009, P.R. China, Jiangsu Center for Drug Screening, China Pharmaceutical University, Nanjing, Jiangsu 210009, P.R. China, Jiangsu Center for Pharmacodynamics Research and Evaluation, China Pharmaceutical University, Nanjing, Jiangsu 210009, P.R. China, Department of Molecular Biology, University of Saarland, D-66421 Homburg, Germany, Department of Complex Prescription of Traditional Chinese Medicine, School of Chinese Material Medicine, China Pharmaceutical University, Nanjing, Jiangsu 210009, P.R. China, Jiangsu Center for Drug Screening, China Pharmaceutical University, Nanjing, Jiangsu 210009, P.R. China
Published online on: February 5, 2013 https://doi.org/10.3892/or.2013.2272
Pages: 1379-1386

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Abstract

The saponin monomer of dwarf lilyturf tuber, DT-13, exhibits anticancer activity by reducing human breast cancer cell adhesion and migration under hypoxia. To further investigate the anticancer activity of DT-13, we investigated whether DT-13 exhibits anti-angiogenic activity. DT-13 showed no effect on human umbilical vein endothelial cell proliferation but inhibited tube formation and migration under normoxia and hypoxia. Moreover, DT-13 significantly reduced density of vessels in vivo observed from a chicken chorioallantoic membrane model. Western blotting results showed that DT-13 suppressed the increased level of hypoxia-inducible factor 1α, p-extracellular signal-regulated kinase 1/2 and p-Akt induced by hypoxia. Enzyme-linked immunosorbent assay revealed that vascular endothelial growth factor excretion was suppressed by DT-13. DT-13 inhibited migration and tube formation induced by vascular endothelial growth factor under normoxia and hypoxia. In addition, DT-13 reduced the level of p-vascular endothelial growth factor receptor 2 and p-Akt induced by vascular endothelial growth factor. Our data suggest that DT-13 inhibits angiogenesis under normoxia and hypoxia and also inhibits angiogenesis induced by vascular endothelial growth factor via targeting at multi elements.

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