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Article

Cordycepin increases sensitivity of Hep3B human hepatocellular carcinoma cells to TRAIL-mediated apoptosis by inactivating the JNK signaling pathway

  • Authors:
    • Hye Hyeon Lee
    • Jin-Woo Jeong
    • Jun Hyuk Lee
    • Gi-Young Kim
    • Jaehun  Cheong
    • Yong Kee Jeong
    • Young Hyun Yoo
    • Yung Hyun Choi
  • View Affiliations / Copyright

    Affiliations: Department of Biotechnology and Medi-Farm Industrialization Research Center, Dong-A University, Busan 604‑714, Republic of Korea, Center for Core Research Facilities, Daegu Gyeongbuk Institute of Science and Technology, Daegu 711-873, Republic of Korea, Biotechnology Examination Division, Chemistry and Biotechnology Examination Bureau, Korean Intellectual Property Office, Daejeon 302-701, Republic of Korea, Laboratory of Immunobiology, Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea, Department of Molecular Biology, College of Natural Sciences, Pusan National University, Busan 609-735, Republic of Korea, Department of Anatomy and Cell Biology and Mitochondria Hub Regulation Center, College of Medicine, Dong-A University, Busan 602-714, Republic of Korea, Department of Biochemistry, Dongeui University College of Oriental Medicine, Busan 614-052, Republic of Korea
  • Pages: 1257-1264
    |
    Published online on: July 4, 2013
       https://doi.org/10.3892/or.2013.2589
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Abstract

Resistance to tumor necrosis factor-related apoptosis‑inducing ligand (TRAIL)-induced apoptosis has been reported in various cancer cells. Cordycepin, a specific polyadenylation inhibitor, is the main functional component in Cordyceps militaris, which possesses many pharmacological activities including antitumor and anti-inflammation. In the present study, we demonstrated that treatment of cordycepin sensitized TRAIL-resistant Hep3B human hepatocellular carcinoma cells to TRAIL-mediated apoptosis as evidenced by formation of apoptotic bodies, chromatin condensation and accumulation of cells in the sub-G1 phase. The induction of apoptosis following co-treatment with cordycepin and TRAIL in Hep3B cells appeared to be correlated with modulation of Bcl-2 family protein expression and activation of the caspase cascade, which resulted in the cleavage of poly(ADP-ribose) polymerase and β-catenin. In addition, cordycepin treatment also inhibited activation of c-Jun N-terminal kinase (JNK). Pretreatment with SP600125, a JNK inhibitor, resulted in a significantly increased sub-G1 population and caspase activity in cordycepin plus TRAIL-mediated apoptosis. Taken together, these results indicate that JNK acts as a key regulator of apoptosis in response to combined treatment with cordycepin and TRAIL in human hepatocellular carcinoma Hep3B cells.
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Copy and paste a formatted citation
Spandidos Publications style
Lee HH, Jeong J, Lee JH, Kim G, Cheong J, Jeong YK, Yoo YH and Choi YH: Cordycepin increases sensitivity of Hep3B human hepatocellular carcinoma cells to TRAIL-mediated apoptosis by inactivating the JNK signaling pathway. Oncol Rep 30: 1257-1264, 2013.
APA
Lee, H.H., Jeong, J., Lee, J.H., Kim, G., Cheong, J., Jeong, Y.K. ... Choi, Y.H. (2013). Cordycepin increases sensitivity of Hep3B human hepatocellular carcinoma cells to TRAIL-mediated apoptosis by inactivating the JNK signaling pathway. Oncology Reports, 30, 1257-1264. https://doi.org/10.3892/or.2013.2589
MLA
Lee, H. H., Jeong, J., Lee, J. H., Kim, G., Cheong, J., Jeong, Y. K., Yoo, Y. H., Choi, Y. H."Cordycepin increases sensitivity of Hep3B human hepatocellular carcinoma cells to TRAIL-mediated apoptosis by inactivating the JNK signaling pathway". Oncology Reports 30.3 (2013): 1257-1264.
Chicago
Lee, H. H., Jeong, J., Lee, J. H., Kim, G., Cheong, J., Jeong, Y. K., Yoo, Y. H., Choi, Y. H."Cordycepin increases sensitivity of Hep3B human hepatocellular carcinoma cells to TRAIL-mediated apoptosis by inactivating the JNK signaling pathway". Oncology Reports 30, no. 3 (2013): 1257-1264. https://doi.org/10.3892/or.2013.2589
Copy and paste a formatted citation
x
Spandidos Publications style
Lee HH, Jeong J, Lee JH, Kim G, Cheong J, Jeong YK, Yoo YH and Choi YH: Cordycepin increases sensitivity of Hep3B human hepatocellular carcinoma cells to TRAIL-mediated apoptosis by inactivating the JNK signaling pathway. Oncol Rep 30: 1257-1264, 2013.
APA
Lee, H.H., Jeong, J., Lee, J.H., Kim, G., Cheong, J., Jeong, Y.K. ... Choi, Y.H. (2013). Cordycepin increases sensitivity of Hep3B human hepatocellular carcinoma cells to TRAIL-mediated apoptosis by inactivating the JNK signaling pathway. Oncology Reports, 30, 1257-1264. https://doi.org/10.3892/or.2013.2589
MLA
Lee, H. H., Jeong, J., Lee, J. H., Kim, G., Cheong, J., Jeong, Y. K., Yoo, Y. H., Choi, Y. H."Cordycepin increases sensitivity of Hep3B human hepatocellular carcinoma cells to TRAIL-mediated apoptosis by inactivating the JNK signaling pathway". Oncology Reports 30.3 (2013): 1257-1264.
Chicago
Lee, H. H., Jeong, J., Lee, J. H., Kim, G., Cheong, J., Jeong, Y. K., Yoo, Y. H., Choi, Y. H."Cordycepin increases sensitivity of Hep3B human hepatocellular carcinoma cells to TRAIL-mediated apoptosis by inactivating the JNK signaling pathway". Oncology Reports 30, no. 3 (2013): 1257-1264. https://doi.org/10.3892/or.2013.2589
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