High CCR6/CCR7 expression and Foxp3+ Treg cell number are positively related to the progression of laryngeal squamous cell carcinoma

  • Authors:
    • Bin Chen
    • Duo Zhang
    • Jian Zhou
    • Qing Li
    • Lin Zhou
    • Shi-Min Li
    • Li Zhu
    • Kuang-Yen Chou
    • Liang Zhou
    • Lei Tao
    • Li-Ming Lu
  • View Affiliations

  • Published online on: July 8, 2013     https://doi.org/10.3892/or.2013.2603
  • Pages: 1380-1390
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Abstract

Chemokine receptors CCR6 and CCR7 have been reported to play important roles in T cell migration and organ-specific metastasis of various tumors. In the present study, we evaluated the expression and clinical significance of CCR6, CCR7, their ligands and CD4+CD25+Foxp3+ regulatory T cells in laryngeal squamous cell carcinoma (LSCC) and metastatic lymph nodes (LNs). The expression of CCR6, CCR7 and their ligands mRNA (CCL20, CCL19/CCL21) as well as the CCR6 and CCR7 proteins were detected by real-time RT-PCR and immunohistochemistry (IHC), respectively. Flow cytometry was used to investigate the percentage of CD4+CD25+Foxp3+ regulatory T cells (Tregs) in peripheral blood mononuclear cells (PBMCs). Furthermore, a number of cytokines, including interleukin (IL)-2, IL-4, IL-10, IL-12p70, interferon (IFN)-γ and transforming growth factor (TGF)-β1 were detected by ELISA. The results showed that CCR6 and CCR7 were expressed in tumors in situ, metastatic LNs and CD4+CD25+Foxp3+ Tregs. It was hypothesized that the expression profile of CCR6, CCR7 and the proliferation of CD4+CD25+Foxp3+ Tregs affected the process of LN metastasis in LSCC patients. Therefore, the increased percentage of the Foxp3+ Tregs and the upregulation of Foxp3 expression on CCR6+ Tregs in LSCC patients may have accounted for the downregulation of antitumor immunity in these patients, which could be valuable for assessment of prognosis in LSCC treatment.
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September 2013
Volume 30 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Chen B, Zhang D, Zhou J, Li Q, Zhou L, Li S, Zhu L, Chou K, Zhou L, Tao L, Tao L, et al: High CCR6/CCR7 expression and Foxp3+ Treg cell number are positively related to the progression of laryngeal squamous cell carcinoma. Oncol Rep 30: 1380-1390, 2013.
APA
Chen, B., Zhang, D., Zhou, J., Li, Q., Zhou, L., Li, S. ... Lu, L. (2013). High CCR6/CCR7 expression and Foxp3+ Treg cell number are positively related to the progression of laryngeal squamous cell carcinoma. Oncology Reports, 30, 1380-1390. https://doi.org/10.3892/or.2013.2603
MLA
Chen, B., Zhang, D., Zhou, J., Li, Q., Zhou, L., Li, S., Zhu, L., Chou, K., Zhou, L., Tao, L., Lu, L."High CCR6/CCR7 expression and Foxp3+ Treg cell number are positively related to the progression of laryngeal squamous cell carcinoma". Oncology Reports 30.3 (2013): 1380-1390.
Chicago
Chen, B., Zhang, D., Zhou, J., Li, Q., Zhou, L., Li, S., Zhu, L., Chou, K., Zhou, L., Tao, L., Lu, L."High CCR6/CCR7 expression and Foxp3+ Treg cell number are positively related to the progression of laryngeal squamous cell carcinoma". Oncology Reports 30, no. 3 (2013): 1380-1390. https://doi.org/10.3892/or.2013.2603