Proteomic identification of target proteins following Drosha knockdown in cervical cancer

  • Authors:
    • Jun Zhou
    • Jing Cai
    • Zaiju Huang
    • Hui Ding
    • Junjie Wang
    • Jinghui Jia
    • Yong Zhao
    • Da Huang
    • Zehua Wang
  • View Affiliations

  • Published online on: August 20, 2013     https://doi.org/10.3892/or.2013.2672
  • Pages: 2229-2237
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Abstract

The nuclear microRNA (miRNA) processing enzyme Drosha is upregulated in cervical cancer, and its overexpression is related to an invasive tumour phenotype. However, the mechanisms that underlie this effect remain poorly understood. The aim of this study was to identify the potential targets of Drosha in cervical cancer. Here, we demonstrated that Drosha knockdown (Drosha-KD) inhibited proliferation, colony formation and the migration of cervical cancer cells in vitro. A global upregulation of proteins in Drosha-KD cells was revealed by two-dimensional gel electrophoresis (2-DE). Eighteen proteins were identified by liquid chromatography and tandem mass spectrometry technology (LC-MS/MS) from 21 selected protein spots that exhibited significant alterations in Drosha-KD cells. The majority of the identified proteins have been previously associated with tumour formation. The downregulation of tubulin 5β in Drosha-KD cervical cancer cells was further confirmed by western blotting. Our results suggest that Drosha affects the biological activity of cervical cancer cells by regulating the expression of numerous tumour-associated proteins.
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November 2013
Volume 30 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Zhou J, Cai J, Huang Z, Ding H, Wang J, Jia J, Zhao Y, Huang D and Wang Z: Proteomic identification of target proteins following Drosha knockdown in cervical cancer. Oncol Rep 30: 2229-2237, 2013.
APA
Zhou, J., Cai, J., Huang, Z., Ding, H., Wang, J., Jia, J. ... Wang, Z. (2013). Proteomic identification of target proteins following Drosha knockdown in cervical cancer. Oncology Reports, 30, 2229-2237. https://doi.org/10.3892/or.2013.2672
MLA
Zhou, J., Cai, J., Huang, Z., Ding, H., Wang, J., Jia, J., Zhao, Y., Huang, D., Wang, Z."Proteomic identification of target proteins following Drosha knockdown in cervical cancer". Oncology Reports 30.5 (2013): 2229-2237.
Chicago
Zhou, J., Cai, J., Huang, Z., Ding, H., Wang, J., Jia, J., Zhao, Y., Huang, D., Wang, Z."Proteomic identification of target proteins following Drosha knockdown in cervical cancer". Oncology Reports 30, no. 5 (2013): 2229-2237. https://doi.org/10.3892/or.2013.2672