Antitumor effect of 1, 8-cineole against colon cancer

Murata,Soichiro; Shiragami,Risa; Kosugi,Chihiro; Tezuka,Tohru; Yamazaki,Masato; Hirano,Atsushi; Yoshimura,Yukino; Suzuki,Masato; Shuto,Kiyohiko; Ohkohchi,Nobuhiro; Koda,Keiji

doi:10.3892/or.2013.2763

Antitumor effect of 1, 8-cineole against colon cancer

Authors:
- Soichiro Murata
- Risa Shiragami
- Chihiro Kosugi
- Tohru Tezuka
- Masato Yamazaki
- Atsushi Hirano
- Yukino Yoshimura
- Masato Suzuki
- Kiyohiko Shuto
- Nobuhiro Ohkohchi
- Keiji Koda
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Affiliations: Department of Surgery, Teikyo University Chiba Medical Center, Ichihara, Japan, Department of Surgery, Division of Gastroenterological and Hepatobiliary Surgery, and Organ Transplantation, University of Tsukuba, Tsukuba, Ibaraki, Japan
Published online on: October 1, 2013 https://doi.org/10.3892/or.2013.2763
Pages: 2647-2652

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Abstract

Several essential oils possess pharmacological effects. Among the various constituents of essential oils, 1, 8-cineole has been shown to possess pharmacological effects such as anti-bacterial and anti-inflammatory effects. The effect of 1, 8-cineole on human colorectal cancer cells, however, has not reported previously. In this study, we have investigated the anti-proliferative effect of 1, 8-cineole on human colon cancer cell lines HCT116 and RKO by WST-8 and BrdU assays. The cytotoxicity of 1, 8-cineole was investigated by LDH activity and TUNEL staining. The mechanism of apoptosis by 1, 8-cineole was determined by western blot analyses. In in vivo study, RKO cells were injected into the SCID mice and the effect of 1, 8-cineole was investigated. Specific induction of apoptosis, not necrosis, was observed in human colon cancer cell lines HCT116 and RKO by 1, 8-cineole. The treatment with 1, 8-cineole was associated with inactivation of survivin and Akt and activation of p38. These molecules induced cleaved PARP and caspase-3, finally causing apoptosis. In xenotransplanted SCID mice, the 1, 8-cineole group showed significantly inhibited tumor progression compared to the control group. These results indicated 1, 8-cineole suppressed human colorectal cancer proliferation by inducing apoptosis. Based on these studies 1, 8-cineole would be an effective strategy to treat colorectal cancer.

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