Curcumin suppresses cell proliferation through inhibition of the Wnt/β-catenin signaling pathway in medulloblastoma

He,Misi; Li,Yu; Zhang,Li; Li,Longjiang; Shen,Yi; Lin,Ling; Zheng,Weiping; Chen,Li; Bian,Xiuwu; Ng,Ho-Keung; Tang,Li

doi:10.3892/or.2014.3206

Curcumin suppresses cell proliferation through inhibition of the Wnt/β-catenin signaling pathway in medulloblastoma

Authors:
- Misi He
- Yu Li
- Li Zhang
- Longjiang Li
- Yi Shen
- Ling Lin
- Weiping Zheng
- Li Chen
- Xiuwu Bian
- Ho-Keung Ng
- Li Tang
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Affiliations: Department of Pathophysiology, Chongqing Medical University, Chongqing, P.R. China, Department of Pathology, Insititute of Neuroscience, Chongqing Medical University, Chongqing, P.R. China, Experimental and Teaching Center, Chongqing Medical University, Chongqing, P.R. China, Institute of Pathology and Southwest Cancer Center, Southwest Hospital, Third Military Medical University, Chongqing, P.R. China, Department of Anatomical and Cellular Pathology, The Chinese University of Hong Kong, Hong Kong, SAR, P.R. China
Published online on: May 22, 2014 https://doi.org/10.3892/or.2014.3206
Pages: 173-180

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Abstract

Recently, the survival rate of medulloblastoma patients has greatly improved; yet, patients undergoing current treatment regimes suffer from serious therapy-related side-effects. The aim of the present study was to investigate the anticancer effects of curcumin on medulloblastoma cells by testing its capacity to suppress proliferation and regulate the Wnt/β-catenin pathway. In the present study, cell proliferation was determined by MTT assay. Cell cycle was observed by flow cytometry. The changes in the Wnt/β-catenin pathway were analyzed by immunofluorescence, western blot analysis and RT-PCR. Curcumin treatment resulted in a dose- and time-dependent inhibition of proliferation in the medulloblastoma cell line. Curcumin treatment arrested the cell-cycle at the G2/M phase. Furthermore, curcumin treatment led to activation of GSK-3β, reduced expression of β-catenin and its downstream target cyclin D1. The attenuation of the Wnt/β‑catenin pathway was due to the loss of nuclear β-catenin. In conclusion, curcumin can inhibit cell growth by suppressing the Wnt/β-catenin signaling pathway, and it has the potential to be developed as a therapeutic agent for medulloblastoma.

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