Galectin fingerprinting in naso-sinusal diseases

Duray,Anaëlle; De Maesschalck,Thibault; Decaestecker,Christine; Remmelink,Myriam; Chantrain,Gilbert; Neiveyans,Jennifer; Horoi,Mihaela; Leroy,Xavier; Gabius,Hans-Joachim; Saussez,Sven

doi:10.3892/or.2014.3213

Galectin fingerprinting in naso-sinusal diseases

Authors:
- Anaëlle Duray
- Thibault De Maesschalck
- Christine Decaestecker
- Myriam Remmelink
- Gilbert Chantrain
- Jennifer Neiveyans
- Mihaela Horoi
- Xavier Leroy
- Hans-Joachim Gabius
- Sven Saussez
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Affiliations: Laboratory of Anatomy, Faculty of Medicine and Pharmacy, University of Mons, Mons, Belgium, Department of Oto-Rhino-Laryngology, CHU Saint-Pierre, Université Libre de Bruxelles, Brussels, Belgium, Laboratory of Image, Signal Processing and Acoustics (LISA), Ecole Polytechnique de Bruxelles, Université Libre de Bruxelles, Brussels, Belgium , Department of Pathology, Hôpital Erasme, Université Libre de Bruxelles, Brussels, Belgium, Department of Pathology, Faculty of Medicine, Hôpital Claude Huriez and Centre de Biologie-Pathologie, CHRU, Lille, France, Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig Maximilian University of Munich, Munich, Germany
Published online on: May 23, 2014 https://doi.org/10.3892/or.2014.3213
Pages: 23-32
Copyright: © Duray et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Galectins, a family of endogenous lectins, are multifunctional effectors that act at various sites and can be used in immunohistochemical localization studies of diseased states. Since they form a potentially cooperative and antagonistic network, we tested the hypothesis that histopathological fingerprinting of galectins could refine the molecular understanding of naso-sinusal pathologies. Using non-cross-reactive antibodies against galectin-1, -3, -4, -7, -8 and -9, we characterized the galectin profiles in chronic rhinosinusitis, nasal polyposis, inverted papillomas and squamous cell carcinomas. The expression, signal location and quantitative parameters describing the percentage of positive cells and labeling intensity were assessed for various cases. We discovered that inverted papillomas showed a distinct galectin immunohistochemical profile. Indeed, epithelial overexpression of galectin-3 (p=0.0002), galectin-4 (p<10-6), galectin-7 (p<10-6) and galectin-9 (p<10-6) was observed in inverted papillomas compared to non-malignant diseases. Regarding carcinomas, we observed increased expression of galectin-9 (p<10-6) in epithelial cells compared to non-tumor pathologies. Our results suggest that galectin-3, -4, -7 and -9 could be involved in the biology of inverted papillomas. In addition, we observed that the expression of galectin in naso-sinusal diseases seems to be affected by tumor progression and not inflammatory or allergic phenomena.

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1	Gabius HJ: The Sugar Code Fundamentals of Glycosciences. Wiley-VCH; Weinheim, Germany: 2009
2	Sperandio M: Selectins and glycosyltransferases in leukocyte rolling in vivo. FEBS J. 273:4377–4389. 2006. View Article : Google Scholar : PubMed/NCBI
3	André S, Sanchez-Ruderisch H, Nakagawa H, Buchholz M, Kopitz J, Forberich P, Kemmner W, Böck C, Deguchi K, Detjen KM, Wiedenmann B, von Knebel Doeberitz M, Gress TM, Nishimura S, Rosewicz S and Gabius HJ: Tumor suppressor p16^INK4a- modulator of glycomic profile and galectin-1 expression to increase susceptibility to carbohydrate-dependent induction of anoikis in pancreatic carcinoma cells. FEBS J. 274:3233–3256. 2007.
4	Sanchez-Ruderisch H, Fischer C, Detjen KM, Welzel M, Wimmel A, Manning JC, André S and Gabius HJ: Tumor suppressor p16^INK4a: downregulation of galectin-3, an endogenous competitor of the pro-anoikis effector galectin-1, in a pancreatic carcinoma model. FEBS J. 277:3552–3563. 2010.
5	Wu G, Lu ZH, Gabius HJ, Ledeen RW and Bleich D: Ganglioside GM1 deficiency in effector T cells from NOD mice induces resistance to regulatory T cell suppression. Diabetes. 60:2341–2349. 2011. View Article : Google Scholar : PubMed/NCBI
6	Amano M, Eriksson H, Manning JC, Detjen KM, André S, Nishimura SI, Lehtiö J and Gabius HJ: Tumour suppressor p16^INK4a: anoikis-favouring decrease in N/O-glycan/cell surface sialylation by down-regulation of enzymes in sialic acid biosynthesis in tandem in a pancreatic carcinoma model. FEBS J. 279:4062–4080. 2012.PubMed/NCBI
7	Clark MC and Baum LG: T cells modulate glycans on CD43 and CD45 during development and activation, signal regulation, and survival. Ann NY Acad Sci. 1253:58–67. 2012. View Article : Google Scholar : PubMed/NCBI
8	Liu FT, Yang RY and Hsu DK: Galectins in acute and chronic inflammation. Ann NY Acad Sci. 1253:80–91. 2012. View Article : Google Scholar : PubMed/NCBI
9	Cooper DN: Galectinomics: finding themes in complexity. Biochim Biophys Acta. 1572:209–231. 2002. View Article : Google Scholar : PubMed/NCBI
10	Kaltner H and Gabius HJ: A toolbox of lectins for translating the sugar code: the galectin network in phylogenesis and tumors. Histol Histopathol. 27:397–416. 2012.PubMed/NCBI
11	Haudek KC, Spronk KJ, Voss PG, Patterson RJ, Wang JL and Arnoys EJ: Dynamics of galectin-3 in the nucleus and cytoplasm. Biochim Biophys Acta. 1800.181–189. 2010.PubMed/NCBI
12	Smetana K Jr, Andre S, Kaltner H, Kopitz J and Gabius HJ: Context-dependent multifunctionality of galectin-1: a challenge for defining the lectin as therapeutic target. Expert Opin Ther Targets. 17:379–392. 2013. View Article : Google Scholar : PubMed/NCBI
13	Saussez S, Cucu DR, Decaestecker C, Chevalier D, Kaltner H, André S, Wacreniez A, Toubeau G, Camby I, Gabius HJ and Kiss R: Galectin-7 (p53-induced gene-1): a new prognostic predictor of recurrence and survival in stage IV hypopharyngeal cancer. Ann Surg Oncol. 13:999–1009. 2006. View Article : Google Scholar : PubMed/NCBI
14	Saussez S, Decaestecker C, Lorfevre F, Chevalier D, Mortuaire G, Kaltner H, André S, Toubeau G, Gabius HJ and Leroy X: Increased expression and altered intracellular distribution of adhesion/growth-regulatory lectins galectins-1 and -7 during tumour progression in hypopharyngeal and laryngeal squamous cell carcinomas. Histopathology. 52:483–493. 2008. View Article : Google Scholar
15	Saussez S, Decaestecker C, Mahillon V, Cludts S, Capouillez A, Chevalier D, Vet HK, Andre S, Toubeau G, Leroy X and Gabius HJ: Galectin-3 upregulation during tumor progression in head and neck cancer. Laryngoscope. 118:1583–1590. 2008. View Article : Google Scholar : PubMed/NCBI
16	Saussez S, de Leval L, Decaestecker C, Sirtaine N, Cludts S, Duray A, Chevalier D, André S, Gabius HJ, Remmelink M and Leroy X: Galectin fingerprinting in Warthin’s tumors: lectin-bases approach to trace its origin? Histol Histopathol. 25:541–550. 2010.
17	Cludts S, Decaestecker C, Mahillon V, Chevalier D, Kaltner H, André S, Remmelink M, Leroy X, Gabius HJ and Saussez S: Galectin-8 up-regulation during hypopharyngeal and laryngeal tumor progression and comparison with galectin-1, -3 and -7. Anticancer Res. 29:4933–4940. 2009.PubMed/NCBI
18	Remmelink M, de Leval L, Decaestecker C, Duray A, Crompot E, Sirtaine N, André S, Kaltner H, Leroy X, Gabius HJ and Saussez S: Quantitative immunohistochemical fingerprinting of adhesion/growth-regulatory galectins in salivary gland tumours: divergent profiles with diagnostic potential. Histopathology. 58:543–556. 2011. View Article : Google Scholar
19	Liu FT and Rabinovich G: Galectins as modulators of tumour progression. Nat Rev Cancer. 5:29–41. 2005. View Article : Google Scholar : PubMed/NCBI
20	Kaltner H, Raschta AS, Manning JC and Gabius HJ: Copy-number variation of functional galectin genes: studying animal galectin-7 (p53-induced gene 1 in man) and tandem-repeat-type galectins-4 and -9. Glycobiology. 23:1152–1163. 2013. View Article : Google Scholar : PubMed/NCBI
21	Teclu A and Lacroix JS: Chronic rhinosinusitis and nasal polyposis - a review. Otorinolaringol. 53:89–97. 2003.
22	Wood AJ and Douglas RG: Pathogenesis and treatment of chronic rhinosinusitis. Postgrad Med J. 86:359–364. 2010. View Article : Google Scholar : PubMed/NCBI
23	Georgy MS and Peters AT: Chapter 8: Rhinosinusitis. Allergy Asthma Proc. 33:24–27. 2012. View Article : Google Scholar
24	Sok JC and Ferguson BJ: Differential diagnosis of eosinophilic chronic rhinosinusitis. Clin Allergy Immunol. 19:69–85. 2007.PubMed/NCBI
25	Takeno S, Hirakawa K and Ishino T: Pathological mechanisms and clinical features of eosinophilic chronic rhinosinusitis in the Japanese population. Allergol Int. 59:247–256. 2010. View Article : Google Scholar : PubMed/NCBI
26	Ferguson BJ: Categorization of eosinophilic chronic rhinosinusitis. Curr Opin Otolaryngol Head Neck Surg. 12:237–242. 2004. View Article : Google Scholar : PubMed/NCBI
27	Delbrouck C, Gabius HJ, Kaltner H, Decaestecker C, Kiss R and Hassid S: Expression patterns of galectin-1 and galectin-3 in nasal polyps and middle and inferior turbinates in relation to growth regulation and immunosuppression. Arch Otolaryngol Head Neck Surg. 129:665–669. 2003. View Article : Google Scholar : PubMed/NCBI
28	Georgy MS and Peters AT: Chapter 7: Nasal polyps. Allergy Asthma Proc. 33:22–23. 2012. View Article : Google Scholar : PubMed/NCBI
29	Cheng W, Zheng C, Tian J and Shi G: T helper cell population and eosinophilia in nasal polyps. J Investig Allergol Clin Immunol. 17:297–301. 2007.PubMed/NCBI
30	Tomassen P, Van Zele T, Zhang N, Perez-Novo C, Van Bruaene N, Gevaert P and Bachert C: Pathophysiology of chronic rhinosinusitis. Proc Am Thorac Soc. 8:115–120. 2011. View Article : Google Scholar
31	Nadir H, Prades JM, Dumoliard JM and Martin CH: Papillomes inverses des cavités naso-sinusiennes: A propos de 19 patients. Journal Français d’oto-rhino-laryngologie. 52:81–86. 2003.(In French).
32	Anari S and Carrie S: Sinonasal inverted papilloma: narrative review. J Laryngol Otol. 124:705–715. 2010. View Article : Google Scholar : PubMed/NCBI
33	Brasnu D, Ayache D, Hans S, Hartl D and Papon JF: Traité d’ORL. Flammarion, Paris: pp. 212–220. 2008
34	Altavilla G, Staffieri A, Busatto G, Canesso A, Giacomelli L and Marioni G: Expression of p53, p16^INK4A, pRB, p21^WAF1/CIP1, p27^KIP1, cyclin D1, Ki-67 and HPV DNA in sinonasal endophytic Schneiderian (inverted) papilloma. Acta Otolaryngol. 129:1242–1249. 2009.
35	Thompson LDR: Head and Neck Pathology. Churchill Livingstone/Elsevier; Philadelphia: pp. 124–132. pp. 155–160. 2006
36	Kopitz J, André S, von Reitzenstein C, Versluis K, Kaltner H, Pieters RJ, Wasano K, Kuwabara I, Liu FT, Cantz M, Heck AJ and Gabius HJ: Homodimeric galectin-7 (p53-induced gene 1) is a negative growth regulator for human neuroblastoma cells. Oncogene. 22:6277–6288. 2003. View Article : Google Scholar : PubMed/NCBI
37	Purkrábková T, Smetana K Jr, Dvoránková B, Holíková Z, Böck C, Lensch M, André S, Pytlík R, Liu FT, Klíma J, Smetana K, Motlik J and Gabius HJ: New aspects of galectin functionality in nuclei of cultured bone marrow stromal and epidermal cells: biotinylated galectins as tool to detect specific binding sites. Biol Cell. 95:535–545. 2003.PubMed/NCBI
38	Langbein S, Brade J, Badawi JK, Hatzinger M, Kaltner H, Lensch M, Specht K, André S, Brinck U, Alken P and Gabius HJ: Gene-expression signature of adhesion/growth-regulatory tissue lectins (galectins) in transitional cell cancer and its prognostic relevance. Histopathology. 51:681–690. 2007. View Article : Google Scholar : PubMed/NCBI
39	Kaltner H, Kübler D, López-Merino L, Lohr M, Manning JC, Lensch M, Seidler J, Lehmann WD, André S, Solís D and Gabius HJ: Toward comprehensive analysis of the galectin network in chicken: unique diversity of galectin-3 and comparison of its localization profile in organs of adult animals to the other four members of this lectin family. Anat Rec. 294:427–444. 2011. View Article : Google Scholar
40	Fík Z, Valach J, Chovanec M, Mazánek J, Kodet R, Kodet O, Tachezy R, Foltynova E, André S, Kaltner H, Gabius HJ and Smetana K Jr: Loss of adhesion/growth-regulatory galectin-9 from squamous cell epithelium in head and neck carcinomas. J Oral Pathol Med. 42:166–1673. 2013.PubMed/NCBI
41	Saal I, Nagy N, Lensch M, Lohr M, Manning JC, Decaestecker C, André S, Kiss R, Salmon I and Gabius HJ: Human galectin-2: expression profiling by RT-PCR/immunohistochemistry and its introduction as histochemical tool for ligand localization. Histol Histopathol. 20:1191–1208. 2005.PubMed/NCBI
42	Lohr M, Kaltner H, Lensch M, André S, Sinowatz F and Gabius HJ: Cell-type-specific expression of murine multifunctional galectin-3 and its association with follicular atresia/luteolysis in contrast to pro-apoptotic galectins-1 and -7. Histochem Cell Biol. 130:567–581. 2008. View Article : Google Scholar : PubMed/NCBI
43	Sarter K, Janko C, Andre S, Munoz LE, Schorn C, Winkler S, Rech J, Kaltner H, Lorenz HM, Schiller M, Andreoli L, Manfredi AA, Isenberg DA, Schett G, Herrmann M and Gabius HJ: Autoantibodies against galectins are associated with antiphospholipid syndrome in patients with systemic lupus erythematosus. Glycobiology. 23:12–22. 2013. View Article : Google Scholar : PubMed/NCBI
44	Danguy A, Rorive S, Decaestecker C, Bronckart Y, Kaltner H, Hadari YR, Goren R, Zich Y, Petein M, Salmon I, Gabius HJ and Kiss R: Immunohistochemical profile of galectin-8 expression in benign and malignant tumors of epithelial, mesenchymatous and adipous origins, and of the nervous system. Histol Histopathol. 16:861–868. 2001.PubMed/NCBI
45	Wang JL, Gray RM, Haudek KC and Patterson RJ: Nucleocytoplasmic lectins. Biochim Biophys Acta. 1673:75–93. 2004. View Article : Google Scholar : PubMed/NCBI
46	Rubinstein N, Ilarregui JM, Toscano MA and Rabinovich GA: The role of galectins in the initiation, amplification and resolution of the inflammatory response. Tissue Antigens. 64:1–12. 2004. View Article : Google Scholar : PubMed/NCBI
47	Sena AAS, Provazzi PJS, Fernandes AM, Cury PM, Rahal P and Oliani SM: Spatial expression of two anti-inflammatory mediators, annexin 1 and galectin-1, in nasal polyposis. Clin Exp Allergy. 36:1260–1267. 2006. View Article : Google Scholar : PubMed/NCBI
48	Iino Y, Miyazama T, Kakizaki K, Saigusa H, Katano H, Shiga J and Kanegasaki S: Expression of ecalectin, a novel eosinophil chemoattractant, in nasal polyps. Acta Otolaryngol. 126:43–50. 2006. View Article : Google Scholar : PubMed/NCBI
49	Matsuura A, Tsukada J, Mizobe T, Higashi T, Mouri F, Tanikawa R, Yamauchi A, Hirashima M and Tanaka Y: Intracellular galectin-9 activates inflammatory cytokines in monocytes. Genes Cells. 14:511–521. 2009. View Article : Google Scholar : PubMed/NCBI
50	Nobumoto A, Nagahara K, Oomizu S, Katoh S, Nishi N, Takeshita K, Niki T, Tominaga A, Yamauchi A and Hirashima M: Galectin-9 suppresses tumor metastasis by blocking adhesion to endothelium and extracellular matrices. Glycobiology. 18:735–744. 2008. View Article : Google Scholar : PubMed/NCBI
51	Pioche-Durieu C, Keryer C, Souquere S, Bosq J, Faigle W, Loew D, Hirashima M, Nishi N, Middeldorp J and Busson P: In nasopharyngeal carcinoma cells, Epstein-Barr virus LMP1 interacts with galectin 9 in membrane raft elements resistant to simvastatin. J Virol. 79:13326–13337. 2005. View Article : Google Scholar : PubMed/NCBI
52	Keryer-Bibens C, Pioche-Durieu C, Villemant C, Souquere S, Nishi N, Hirashima M, Middeldorp J and Busson P: Exosomes released by EBV-infected nasopharyngeal carcinoma cells convey the viral latent membrane protein 1 and the immunomodulatory protein galectin 9. BMC Cancer. 6:2832006. View Article : Google Scholar
53	Klibi J, Niki T, Riedel A, Pioche-Durieu C, Souquere S, Rubinstein E, Le Moulec S, Guigay J, Hirashima M, Guemira F, Adhikary D, Mautner J and Busson P: Blood diffusion and Th1-suppressive effects of galectin-9-containing exosomes released by Epstein-Barr virus-infected nasopharyngeal carcinoma cells. Blood. 113:1957–1966. 2009. View Article : Google Scholar
54	Hadari YR, Arbel-Goren R, Levy Y, Amsterdam A, Alon R, Zakut R and Zick Y: Galectin-8 binding to integrins inhibits cell adhesion and induces apoptosis. J Cell Sci. 113:2385–2397. 2000.PubMed/NCBI
55	Saussez S, Lorfevre F, Nonclercq D, Laurent G, André S, Journé F, Kiss R, Toubeau G and Gabius HJ: Towards functional glycomics by localization of binding sites for tissue lectins: lectin histochemical reactivity for galectins during diethylstilbestrol-induced kidney tumorigenesis in male Syrian hamster. Histochem Cell Biol. 126:57–69. 2006. View Article : Google Scholar
56	Vansthertem D, Cludts S, Nonclercq D, Gossiaux A, Saussez S, Legrand A, Gabius HJ and Toubeau G: Immunohistochemical localization of galectins-1 and -3 and monitoring of tissue galectin-binding sites during tubular regeneration after renal ischemia reperfusion in the rat. Histol Histopathol. 25:1417–1429. 2010.
57	Dawson H, André S, Karamitopoulou E, Zlobec I and Gabius HJ: The growing galectin network in colon cancer and clinical relevance of cytoplasmic galectin-3 reactivity. Anticancer Res. 33:3053–3059. 2013.PubMed/NCBI