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Cyclooxygenase-2 inhibition as a strategy for treating gastric adenocarcinoma

  • Authors:
    • Hong-Gang Xiang
    • Xiao Xie
    • Feng-Qing Hu
    • Hai-Bo Xiao
    • Wen-Jie Zhang
    • Lei Chen

  • View Affiliations

  • Published online on: July 2, 2014     https://doi.org/10.3892/or.2014.3301
  • Pages: 1140-1148
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Abstract

Cyclooxygenase-2 (COX-2) has been proven to play critical roles in inflammation as well as in cancer. Some studies have shown that the anti-inflammatory, immunosuppressive and anti-arthritic effects of celecoxib are mainly attributed to the inhibition of COX-2 expression. The present study aimed to investigate the function of COX-2 in human gastric adenocarcinoma (GAC). Forty-five cases of human GAC tissues and corresponding adjacent non-cancerous tissues (ANCTs) were collected. The expression of COX-2 and proliferating cell nuclear antigen (PCNA) was assessed using immunohistochemical assay through a tissue microarray procedure. GAC cells (SGC-7901 and MKN-45) in vitro were treated with COX-2 siRNA or different concentrations of celecoxib to observe their effects on cell proliferation, invasion and the underlying molecular mechanisms. As a consequence, the expression of COX-2 and PCNA was found in cancer tissues with a higher strong reactivity rate, compared with the ANCTs (80.0 vs. 53.3%, P=0.011; 68.9 vs. 48.9%, P=0.047), and COX-2 was positively associated with lymph node metastasis of GAC patients (P=0.011). Targeted knockdown of COX-2 inhibited the proliferation, migration and invasion of GAC cells with decreased expression of PCNA. COX-2 inhibitor celecoxib also suppressed the proliferative activities of GAC cells with decreased expression of COX-2 and PCNA. In addition, the tumor volume in the MKN-45 subcutaneous tumor model treated with siCOX-2 was significantly smaller than that of the negative control (NC) group (P<0.01). Taken together, our findings offer a strong preclinical rationale to target COX-2 signaling as a therapeutic strategy to improve the treatment of gastric adenocarcinoma.
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September-2014
Volume 32 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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  • Impact Factor: 3.8
  • Ranked Oncology
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  • CiteScore: 8.5
  • CiteScore Rank: Q1: #74/404 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.719
  • SCImago Journal Rank (SJR): 0.864
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Spandidos Publications style
Xiang H, Xie X, Hu F, Xiao H, Zhang W and Chen L: Cyclooxygenase-2 inhibition as a strategy for treating gastric adenocarcinoma. Oncol Rep 32: 1140-1148, 2014.
APA
Xiang, H., Xie, X., Hu, F., Xiao, H., Zhang, W., & Chen, L. (2014). Cyclooxygenase-2 inhibition as a strategy for treating gastric adenocarcinoma. Oncology Reports, 32, 1140-1148. https://doi.org/10.3892/or.2014.3301
MLA
Xiang, H., Xie, X., Hu, F., Xiao, H., Zhang, W., Chen, L."Cyclooxygenase-2 inhibition as a strategy for treating gastric adenocarcinoma". Oncology Reports 32.3 (2014): 1140-1148.
Chicago
Xiang, H., Xie, X., Hu, F., Xiao, H., Zhang, W., Chen, L."Cyclooxygenase-2 inhibition as a strategy for treating gastric adenocarcinoma". Oncology Reports 32, no. 3 (2014): 1140-1148. https://doi.org/10.3892/or.2014.3301