Broussonetia kazinoki modulates the expression of VEGFR-2 and MMP-2 through the inhibition of ERK, Akt and p70S6K‑dependent signaling pathways: Its implication in endothelial cell proliferation, migration and tubular formation

  • Authors:
    • Young-Rak Cho
    • Jae Hyeon Kim
    • Jin-Kyu Kim
    • Eun-Kyung Ahn
    • Hye-Jin Ko
    • Jae Kyung In
    • Sang-Jin Lee
    • Gyu-Un Bae
    • Yong Kee Kim
    • Joa Sub Oh
    • Dong-Wan Seo
  • View Affiliations

  • Published online on: August 4, 2014     https://doi.org/10.3892/or.2014.3380
  • Pages: 1531-1536
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Abstract

Broussonetia kazinoki (BK) has been used as a traditional medicine to improve vision, as well as for inflammatory and infectious diseases. In the present study, we investigated the effects and molecular mechanism of the ethanolic extract of BK on cell proliferation, migration and tubular formation in vascular endothelial growth factor-A (VEGF-A)-treated human umbilical vein endothelial cells. BK treatment inhibited VEGF-A-stimulated endothelial cell proliferation through the downregulation of cell cycle-related proteins including cyclin-dependent kinases and cyclins. Moreover, BK treatment suppressed cell migration and tubular formation in response to VEGF-A. These anti-angiogenic activities of BK were associated with the inactivation of mitogenic signaling pathways including extracellular signal-regulated kinase, Akt and p70S6K, and the subsequent downregulation of VEGFR-2 and matrix metalloproteinase-2. Taken together, these findings suggest further evaluation and development of BK as a potential therapeutic agent for the treatment and prevention of angiogenesis-related diseases including cancer.
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October 2014
Volume 32 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Cho Y, Kim JH, Kim J, Ahn E, Ko H, In JK, Lee S, Bae G, Kim YK, Oh JS, Oh JS, et al: Broussonetia kazinoki modulates the expression of VEGFR-2 and MMP-2 through the inhibition of ERK, Akt and p70S6K‑dependent signaling pathways: Its implication in endothelial cell proliferation, migration and tubular formation. Oncol Rep 32: 1531-1536, 2014.
APA
Cho, Y., Kim, J.H., Kim, J., Ahn, E., Ko, H., In, J.K. ... Seo, D. (2014). Broussonetia kazinoki modulates the expression of VEGFR-2 and MMP-2 through the inhibition of ERK, Akt and p70S6K‑dependent signaling pathways: Its implication in endothelial cell proliferation, migration and tubular formation. Oncology Reports, 32, 1531-1536. https://doi.org/10.3892/or.2014.3380
MLA
Cho, Y., Kim, J. H., Kim, J., Ahn, E., Ko, H., In, J. K., Lee, S., Bae, G., Kim, Y. K., Oh, J. S., Seo, D."Broussonetia kazinoki modulates the expression of VEGFR-2 and MMP-2 through the inhibition of ERK, Akt and p70S6K‑dependent signaling pathways: Its implication in endothelial cell proliferation, migration and tubular formation". Oncology Reports 32.4 (2014): 1531-1536.
Chicago
Cho, Y., Kim, J. H., Kim, J., Ahn, E., Ko, H., In, J. K., Lee, S., Bae, G., Kim, Y. K., Oh, J. S., Seo, D."Broussonetia kazinoki modulates the expression of VEGFR-2 and MMP-2 through the inhibition of ERK, Akt and p70S6K‑dependent signaling pathways: Its implication in endothelial cell proliferation, migration and tubular formation". Oncology Reports 32, no. 4 (2014): 1531-1536. https://doi.org/10.3892/or.2014.3380