RRM1, TUBB3, TOP2A, CYP19A1, CYP2D6: Difference between mRNA and protein expression in predicting prognosis of breast cancer patients

  • Authors:
    • Ying-Chun Xu
    • Feng-Chun Zhang
    • Jun-Jian Li
    • Jia-Qi Dai
    • Qiang Liu
    • Lei Tang
    • Yue Ma
    • Qi Xu
    • Xiao-Lin Lin
    • Hong-Bin Fan
    • Hong-Xia Wang
  • View Affiliations

  • Published online on: August 7, 2015     https://doi.org/10.3892/or.2015.4183
  • Pages: 1883-1894
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Abstract

The study investigated the clinical significance of RRM1 (ribonucleoside reductase subunit M1), TUBB3 (tubulin-β-III), TOP2A (DNA topoisomerase II), CYP19A1 (cytochrome P450, family 19, subfamily A, polypeptide 1) and CYP2D6 (cytochrome P450, family 2, subfamily D, polypeptide 6) for the diagnosis and possible predictive roles in breast cancer. Tissue microarray detected the expression of RRM1, tubulin-β-III, Topo IIα, CYP19A1 and CYP2D6 protein in breast cancer tissue and tissue adjacent to tumors (TATs). In addition, a publically available tool, was used to assess the prognostic value of their gene expression in breast cancer (http://kmplot.com). Analysis for relapse-free survival (RFS), disease-free survival (DFS) and overall survival (OS) was performed. Cytoplasmic RRM1, tubulin-β-III, CYP19A1 and Topo IIα staining were significantly higher in breast cancer tissues compared with TATs (P<0.050). Significant correlation occurred between RRM1 expression with pathological classification (P=0.018), lymph node involvement (P=0.035) and ER status (P=0.003). Tubulin-β-III and CYP2D6 expression correlated significantly with tumor grade (P=0.021 for tubulin-β-III and P=0.029 for CYP2D6, respectively). Cox analysis showed that the protein expression of CYP2D6, CYP19A1, RRM1, Topo IIα or tubulin-β-III was not an independent prognostic factor. A significant association occurred between RFS and TUBB3, TOP2A, CYP19A1, and CYP2D6 mRNA expression. With CYP19A1 (P<0.001) and CYP2D6 (P<0.001), a high expression was associated with good clinical outcome. Conversely, a low expression of TUBB3 (P<0.001) and TOP2A (P<0.001) was associated with good clinical outcome. TUBB3 (P=0.0004) and TOP2A (P<0.001) were significant prognostic factors in predicting the patient OS. The expression of RRM1, tubulin-β-III, Topo IIα and CYP19A1 in tumor tissues was significantly higher than that in TATs. TUBB3, TOP2A, CYP19A1 and CYP2D6 gene expression, but not protein expression, was associated with patient survival.
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October-2015
Volume 34 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Xu Y, Zhang F, Li J, Dai J, Liu Q, Tang L, Ma Y, Xu Q, Lin X, Fan H, Fan H, et al: RRM1, TUBB3, TOP2A, CYP19A1, CYP2D6: Difference between mRNA and protein expression in predicting prognosis of breast cancer patients. Oncol Rep 34: 1883-1894, 2015.
APA
Xu, Y., Zhang, F., Li, J., Dai, J., Liu, Q., Tang, L. ... Wang, H. (2015). RRM1, TUBB3, TOP2A, CYP19A1, CYP2D6: Difference between mRNA and protein expression in predicting prognosis of breast cancer patients. Oncology Reports, 34, 1883-1894. https://doi.org/10.3892/or.2015.4183
MLA
Xu, Y., Zhang, F., Li, J., Dai, J., Liu, Q., Tang, L., Ma, Y., Xu, Q., Lin, X., Fan, H., Wang, H."RRM1, TUBB3, TOP2A, CYP19A1, CYP2D6: Difference between mRNA and protein expression in predicting prognosis of breast cancer patients". Oncology Reports 34.4 (2015): 1883-1894.
Chicago
Xu, Y., Zhang, F., Li, J., Dai, J., Liu, Q., Tang, L., Ma, Y., Xu, Q., Lin, X., Fan, H., Wang, H."RRM1, TUBB3, TOP2A, CYP19A1, CYP2D6: Difference between mRNA and protein expression in predicting prognosis of breast cancer patients". Oncology Reports 34, no. 4 (2015): 1883-1894. https://doi.org/10.3892/or.2015.4183