The MRTF-A/B function as oncogenes in pancreatic cancer

  • Authors:
    • Zhao Song
    • Zhao Liu
    • Jing Sun
    • Feng-Lei Sun
    • Chuan-Zhi Li
    • Jiu-Zheng Sun
    • Li-You Xu
  • View Affiliations

  • Published online on: October 15, 2015     https://doi.org/10.3892/or.2015.4329
  • Pages: 127-138
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Abstract

Despite evidence that MRTF-A/B, co-activators of serum response factor (SRF), promotes tumor cell invasion and metastasis in cancer, there are no studies describing MRTF-A/B in pancreatic cancer. To clarify involvement of MRTF-A/B expression in pancreatic cancer, we used quantitative reverse transcription-polymerase chain reaction and western blot analysis to detect MRTF-A/B in pancreatic cancer, intraductal papillary mucinous neoplasm (IPMN) and non-neoplastic pancreata. MRTF-A/B expression differs significantly between cancer and non-neoplastic tissues as well as between non-neoplastic tissues and IPMN bulk tissues. Next, we studied the roles of MRTF-A/B in vitro. Overexpression of MRTF-A/B promoted epithelial‑mesenchymal transition (EMT) and generated stem cell-like cells in normal pancreatic cells. We performed quantitative reverse transcription-polymerase chain reaction to detect the level of MRTF-A/B in 19 pancreatic cancer cell lines. We found that their expression was associated with gemcitabine resistance. Like in normal pancreatic cells, MRTF-A/B also promoted EMT and promoted formation of stem cell-like cells in pancreatic cancer and they could regulate microRNA expression associated with EMT and CICs. Finally, to further demonstrate the roles of MRTF-A/B in vivo, we performed nude mouse model of s.c. xenograft and found that overexpression of MRTF-A and MRTF-B promoted pancreatic cancer growth. Elucidating the roles of MRTF-A/B will help us to further understand molecular basis of the disease and offer new gene targets for effective therapies.
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January-2016
Volume 35 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Song Z, Liu Z, Sun J, Sun F, Li C, Sun J and Xu L: The MRTF-A/B function as oncogenes in pancreatic cancer. Oncol Rep 35: 127-138, 2016.
APA
Song, Z., Liu, Z., Sun, J., Sun, F., Li, C., Sun, J., & Xu, L. (2016). The MRTF-A/B function as oncogenes in pancreatic cancer. Oncology Reports, 35, 127-138. https://doi.org/10.3892/or.2015.4329
MLA
Song, Z., Liu, Z., Sun, J., Sun, F., Li, C., Sun, J., Xu, L."The MRTF-A/B function as oncogenes in pancreatic cancer". Oncology Reports 35.1 (2016): 127-138.
Chicago
Song, Z., Liu, Z., Sun, J., Sun, F., Li, C., Sun, J., Xu, L."The MRTF-A/B function as oncogenes in pancreatic cancer". Oncology Reports 35, no. 1 (2016): 127-138. https://doi.org/10.3892/or.2015.4329