131I therapy mediated by sodium/iodide symporter combined with kringle 5 has a synergistic therapeutic effect on glioma

  • Authors:
    • Shuo Shi
    • Min Zhang
    • Rui Guo
    • Miao Zhang
    • Jiajia Hu
    • Yun Xi
    • Ying Miao
    • Biao Li
  • View Affiliations

  • Published online on: November 13, 2015     https://doi.org/10.3892/or.2015.4420
  • Pages: 691-698
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Abstract

Glioblastoma (GBM) is the most common and most aggressive primary brain tumor; the prognosis of patients with GBM remains poor. The sodium/iodide symporter (NIS) can be used to absorb several isotopes, such as 131I for nuclear medicine imaging and radionuclide therapy. Previously, we found that the early growth response-1 (Egr1) promoter had an 131I radiation positive feedback effect on the NIS gene. Kringle 5 (K5), a kringle domain of plasminogen, induced endothelial cell apoptosis. We investigated the effect of K5 combined with the 131I radiation positive feedback effect (Egr1-NIS) for treating malignant U87 glioma cells using a lentiviral vector. We successfully constructed a stable U87 glioma cell line, U87-K5-Egr1-NIS. The radio-inducible Egr1 promoter induced an 131I radiation positive feedback effect absorbed by NIS. Mediated by 131I, K5 increased glioma cell apoptosis; 131I radiation also increased endothelial cell sensitivity to K5-induced apoptosis. The combined therapy had a synergistic effect on the antitumor efficacy of glioma treatment, not only increasing tumor cell apoptosis but also significantly inhibiting tumor cell proliferation and reducing capillary density in U87 glioma tissues.
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February-2016
Volume 35 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Shi S, Zhang M, Guo R, Zhang M, Hu J, Xi Y, Miao Y and Li B: 131I therapy mediated by sodium/iodide symporter combined with kringle 5 has a synergistic therapeutic effect on glioma. Oncol Rep 35: 691-698, 2016.
APA
Shi, S., Zhang, M., Guo, R., Zhang, M., Hu, J., Xi, Y. ... Li, B. (2016). 131I therapy mediated by sodium/iodide symporter combined with kringle 5 has a synergistic therapeutic effect on glioma. Oncology Reports, 35, 691-698. https://doi.org/10.3892/or.2015.4420
MLA
Shi, S., Zhang, M., Guo, R., Zhang, M., Hu, J., Xi, Y., Miao, Y., Li, B."131I therapy mediated by sodium/iodide symporter combined with kringle 5 has a synergistic therapeutic effect on glioma". Oncology Reports 35.2 (2016): 691-698.
Chicago
Shi, S., Zhang, M., Guo, R., Zhang, M., Hu, J., Xi, Y., Miao, Y., Li, B."131I therapy mediated by sodium/iodide symporter combined with kringle 5 has a synergistic therapeutic effect on glioma". Oncology Reports 35, no. 2 (2016): 691-698. https://doi.org/10.3892/or.2015.4420