β-lapachone suppresses the proliferation of human malignant melanoma cells by targeting specificity protein 1

  • Authors:
    • Woong Bang
    • Young-Joo Jeon
    • Jin Hyoung Cho
    • Ra Ham Lee
    • Seon-Min Park
    • Jae-Cheon Shin
    • Nag-Jin Choi
    • Yung Hyun Choi
    • Jung-Jae Cho
    • Jae-Min Seo
    • Seung-Yeop Lee
    • Jung-Hyun Shim
    • Jung-Il Chae
  • View Affiliations

  • Published online on: November 20, 2015     https://doi.org/10.3892/or.2015.4439
  • Pages: 1109-1116
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

β-lapachone (β-lap), a novel natural quinone derived from the bark of the Pink trumpet tree (Tabebuia avellanedae) has been demonstrated to have anticancer activity. In this study, we investigated whether β-lap exhibits anti-proliferative effects on two human malignant melanoma (HMM) cell lines, G361 and SK-MEL-28. The effects of β-lap on the HMM cell lines were investigated using 3-(4,5-dimethylthiazol-2-yl)‑5-(3-carboxymethoxyphenyl)‑2-(4-sulfophenyl-2H-tetrazolium (MTS) assay, 4',6-diamidino-2-phenylindole (DAPI) staining, Annexin V and Dead cell assay, mitochondrial membrane potential (MMP) assay and western blot analysis. We demonstrated that β-lap significantly induced apoptosis and suppressed cell viability in the HMM cells. Intriguingly, the transcription factor specificity protein 1 (Sp1) was significantly downregulated by β-lap in a dose- and time-dependent manner. Furthermore, β-lap modulated the protein expression level of the Sp1 regulatory genes including cell cycle regulatory proteins and apoptosis-associated proteins. Taken together, our findings indicated that β-lap modulates Sp1 transactivation and induces apoptotic cell death through the regulation of cell cycle- and apoptosis-associated proteins. Thus, β-lap may be used as a promising anticancer drug for cancer prevention and may improve the clinical outcome of patients with cancer.
View Figures
View References

Related Articles

Journal Cover

February-2016
Volume 35 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Bang W, Jeon Y, Cho JH, Lee RH, Park S, Shin J, Choi N, Choi YH, Cho J, Seo J, Seo J, et al: β-lapachone suppresses the proliferation of human malignant melanoma cells by targeting specificity protein 1. Oncol Rep 35: 1109-1116, 2016.
APA
Bang, W., Jeon, Y., Cho, J.H., Lee, R.H., Park, S., Shin, J. ... Chae, J. (2016). β-lapachone suppresses the proliferation of human malignant melanoma cells by targeting specificity protein 1. Oncology Reports, 35, 1109-1116. https://doi.org/10.3892/or.2015.4439
MLA
Bang, W., Jeon, Y., Cho, J. H., Lee, R. H., Park, S., Shin, J., Choi, N., Choi, Y. H., Cho, J., Seo, J., Lee, S., Shim, J., Chae, J."β-lapachone suppresses the proliferation of human malignant melanoma cells by targeting specificity protein 1". Oncology Reports 35.2 (2016): 1109-1116.
Chicago
Bang, W., Jeon, Y., Cho, J. H., Lee, R. H., Park, S., Shin, J., Choi, N., Choi, Y. H., Cho, J., Seo, J., Lee, S., Shim, J., Chae, J."β-lapachone suppresses the proliferation of human malignant melanoma cells by targeting specificity protein 1". Oncology Reports 35, no. 2 (2016): 1109-1116. https://doi.org/10.3892/or.2015.4439