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miR-497 suppresses angiogenesis in breast carcinoma by targeting HIF-1α

  • Authors:
    • Zhihao Wu
    • Xuehong Cai
    • Chenggang Huang
    • Jia Xu
    • An Liu

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  • Published online on: December 29, 2015     https://doi.org/10.3892/or.2015.4529
  • Pages: 1696-1702
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
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Abstract

Angiogenesis is a key factor in the growth and dissemination of malignant diseases, including breast cancer, with significant implications for its clinical management. It is known that microRNAs (miRNAs) play important roles in regulating tumor properties in cancers. However, whether miR-497 contributes to breast cancer angiogenesis remains unknown. Our study demonstrated that miR-497 was significantly downregulated in breast cancer tissue samples and cell lines. Conditioned medium obtained from breast cancer cell line MCF-7, treated with miR-497 mimics, suppressed the proliferation and tube formation of human umbilical vein endothelial cells in vitro, in comparison with the untransfected cells or cells transfected with the control vector alone. Furthermore, western blot assay confirmed that the overexpression of miR-497 reduced VEGF and HIF-1α protein levels. In addition, stable transfection of miR-497 inhibited tumorigenicity and angiogenesis in vivo. Moreover, HIF-1α was also increased in the breast cancer cells under a hypoxic condition, while the ectopic expression of miR-497 partially restored its level. Taken together, our findings indicate that miR-497 is a potential target for the biological therapy of breast cancer. Moreover, miR-497 inhibited the growth of tumors and reduced angiogenesis in a nude mouse xenograft tumor model, which was probably caused by the downregulation of pro-angiogenic molecules, such as VEGF and HIF-1α.
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March-2016
Volume 35 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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  • Impact Factor: 3.8
  • Ranked Oncology
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  • CiteScore: 8.5
  • CiteScore Rank: Q1: #74/404 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.719
  • SCImago Journal Rank (SJR): 0.864
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Spandidos Publications style
Wu Z, Cai X, Huang C, Xu J and Liu A: miR-497 suppresses angiogenesis in breast carcinoma by targeting HIF-1α. Oncol Rep 35: 1696-1702, 2016.
APA
Wu, Z., Cai, X., Huang, C., Xu, J., & Liu, A. (2016). miR-497 suppresses angiogenesis in breast carcinoma by targeting HIF-1α. Oncology Reports, 35, 1696-1702. https://doi.org/10.3892/or.2015.4529
MLA
Wu, Z., Cai, X., Huang, C., Xu, J., Liu, A."miR-497 suppresses angiogenesis in breast carcinoma by targeting HIF-1α". Oncology Reports 35.3 (2016): 1696-1702.
Chicago
Wu, Z., Cai, X., Huang, C., Xu, J., Liu, A."miR-497 suppresses angiogenesis in breast carcinoma by targeting HIF-1α". Oncology Reports 35, no. 3 (2016): 1696-1702. https://doi.org/10.3892/or.2015.4529