UV-dependent phosphorylation of COP9/signalosome in UV-induced apoptosis

  • Authors:
    • Emilie Laure Dubois
    • Scott Gerber
    • Alexei Kisselev
    • Annick Harel-Bellan
    • Regina Groisman
  • View Affiliations

  • Published online on: March 11, 2016     https://doi.org/10.3892/or.2016.4671
  • Pages: 3101-3105
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Abstract

The COP9/signalosome (CSN) multi-protein complex regulates the activity of cullin-RING ubiquitin ligases (CRLs), including the DDB2 and CSA CRL4 ligases (CRL4DDB2 and CRL4CSA), which are involved in the repair of UV-induced DNA damages. In the present study, we demonstrated that the protein kinase ATM, a key component of the DNA damage response (DDR), phosphorylates CSN1 and CSN7a, two subunits of the CSN complex, in a UV-dependent manner. The phosphorylation of CSN1 on serine 474 was detected as early as 3 h after UV-exposure, peaked at 8 h and persisted until 48 h post-UV irradiation. Such a time course suggests a role in late DDR rather than in DNA repair. Consistently, overexpression of a phosphorylation-resistant S474A CSN1 mutant reduced UV-induced apoptosis. Thus, CSN1 appears to play a role not only in DNA repair but also in UV-induced apoptosis.
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May-2016
Volume 35 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Dubois EL, Gerber S, Kisselev A, Harel-Bellan A and Groisman R: UV-dependent phosphorylation of COP9/signalosome in UV-induced apoptosis. Oncol Rep 35: 3101-3105, 2016.
APA
Dubois, E.L., Gerber, S., Kisselev, A., Harel-Bellan, A., & Groisman, R. (2016). UV-dependent phosphorylation of COP9/signalosome in UV-induced apoptosis. Oncology Reports, 35, 3101-3105. https://doi.org/10.3892/or.2016.4671
MLA
Dubois, E. L., Gerber, S., Kisselev, A., Harel-Bellan, A., Groisman, R."UV-dependent phosphorylation of COP9/signalosome in UV-induced apoptosis". Oncology Reports 35.5 (2016): 3101-3105.
Chicago
Dubois, E. L., Gerber, S., Kisselev, A., Harel-Bellan, A., Groisman, R."UV-dependent phosphorylation of COP9/signalosome in UV-induced apoptosis". Oncology Reports 35, no. 5 (2016): 3101-3105. https://doi.org/10.3892/or.2016.4671