Depletion of G9a gene induces cell apoptosis in human gastric carcinoma

  • Authors:
    • Xiaolei Lin
    • Yiqun Huang
    • Yong Zou
    • Xingsheng Chen
    • Xudong Ma
  • View Affiliations

  • Published online on: March 17, 2016     https://doi.org/10.3892/or.2016.4692
  • Pages: 3041-3049
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Abstract

G9a is a mammalian histone methyltransferase that contributes to the epigenetic silencing of tumor suppressor genes. Evidence suggests that G9a is required to maintain the malignant phenotype, but little documentation show the role of G9a function in mediating tumor growth. We retrospectively analyzed the protein of G9a and monomethylated histone H3 lysine 9 (H3K9 me1), and dimethylated histone H3 lysine 9 (H3K9 me2) in 175 cases of gastric carcinoma by immunohistochemistry. RNAi-based inhibition of G9a in MGC803 cancer cell line was studied. G9a depletion was done by transient transfection using Lipofectamine 2000. Depletion efficiency of G9a was tested using real-time PCR and western blot analysis. Cell apoptosis and proliferation were detected by TUNEL assay and MTT, respectively. The proteins of H3K9 me1, me2, trimethylation of H3K9 (H3K9 me3), monomethylated histone H3 lysine 27 (H3K27 me1), dimethylated histone H3 lysine 27 (H3K27 me2) and histone acetylated H3, apoptotic proteins were studied by western blot analysis. G9a and H3K9 me2 expression was higher in gastric cancer cells compared to the control (p<0.05). Both G9a and H3K9 me2 were positively correlated with the degree of differentiation, depth of infiltration, lymphatic invasions and tumor-node-metastasis stage in gastric carcinoma, (p<0.05). RNAi-mediated knockdown of G9a induced cell apoptosis and inhibited cell proliferation. Depletion of G9a reduced the levels of H3K9 me1 and me2, H3K27 me1 and me2. Nonetheless, it did not activate acetylation of H3 and H3K9 me3. These data suggest that G9a is required in tumorigenesis, and correlated with prognosis. Furthermore, G9a plays a critical role in regulating epigenetics. Depletion of G9a inhibits cell growth and induces cells apoptosis in gastric cancer. It might be of therapeutic benefit in gastric cancers.
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May-2016
Volume 35 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Lin X, Huang Y, Zou Y, Chen X and Ma X: Depletion of G9a gene induces cell apoptosis in human gastric carcinoma. Oncol Rep 35: 3041-3049, 2016.
APA
Lin, X., Huang, Y., Zou, Y., Chen, X., & Ma, X. (2016). Depletion of G9a gene induces cell apoptosis in human gastric carcinoma. Oncology Reports, 35, 3041-3049. https://doi.org/10.3892/or.2016.4692
MLA
Lin, X., Huang, Y., Zou, Y., Chen, X., Ma, X."Depletion of G9a gene induces cell apoptosis in human gastric carcinoma". Oncology Reports 35.5 (2016): 3041-3049.
Chicago
Lin, X., Huang, Y., Zou, Y., Chen, X., Ma, X."Depletion of G9a gene induces cell apoptosis in human gastric carcinoma". Oncology Reports 35, no. 5 (2016): 3041-3049. https://doi.org/10.3892/or.2016.4692