LSD1-mediated epigenetic modification contributes to ovarian cancer cell migration and invasion

Li,Yuanxia; Wan,Xiaolei; Wei,Ye; Liu,Xiuwen; Lai,Wensheng; Zhang,Liuping; Jin,Jie; Wu,Chaoyang; Shao,Qixiang; Shao,Genbao; Lin,Qiong

doi:10.3892/or.2016.4729

LSD1-mediated epigenetic modification contributes to ovarian cancer cell migration and invasion

Authors:
- Yuanxia Li
- Xiaolei Wan
- Ye Wei
- Xiuwen Liu
- Wensheng Lai
- Liuping Zhang
- Jie Jin
- Chaoyang Wu
- Qixiang Shao
- Genbao Shao
- Qiong Lin
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Affiliations: School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, P.R. China, Department of Oncology, The Affiliated People's Hospital, Jiangsu University, Zhenjiang, Jiangsu 212002, P.R. China, Department of General Medicine, The Affiliated Third People's Hospital of Jiangsu University, Zhenjiang, Jiangsu 212005, P.R. China
Published online on: April 4, 2016 https://doi.org/10.3892/or.2016.4729
Pages: 3586-3592

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Abstract

Lysine-specific demethylase 1 (LSD1) has been implicated in the process of tumor progression at various steps, but its role in epithelial-messenchymal transition (EMT) and the migration of ovarian cancer cells remains obscure. In this study, we demonstrated the effect of LSD1 on ovarian cancer cell migration and the regulatory role of LSD1 in the expression of EMT markers. Inhibition of LSD1 expression impaired the migration and invasion of HO8910 ovarian cancer cells. In contrast, overexpression of LSD1 enhanced the cell migration and invasion of HO8910 cells. Mechanistic analyses showed that LSD1 promoted cell migration through induction of N-cadherin, vimentin, MMP-2 and inhibition of E-cadherin. Furthermore, LSD1 interacted with the promoter of E-cadherin and demethylated histone H3 lysine 4 (H3K4) at this region, downregulated E-cadherin expression, and consequently enhanced ovarian cancer cell migration. These data indicate that LSD1 acts as an epigenetic regulator of EMT and contributes to the metastasis of ovarian cancer.

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