CCY-1a-E2 induces G2/M phase arrest and apoptotic cell death in HL-60 leukemia cells through cyclin-dependent kinase 1 signaling and the mitochondria-dependent caspase pathway

  • Authors:
    • Chin‑Fen Lin
    • Jai‑Sing Yang
    • Chingju Lin
    • Fuu‑Jen Tsai
    • Chi‑Cheng Lu
    • Miau‑Rong Lee
  • View Affiliations

  • Published online on: July 25, 2016     https://doi.org/10.3892/or.2016.4970
  • Pages: 1633-1639
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Abstract

Our previous study demonstrated that 2-[(3-methoxybenzyl)oxy]benzaldehyde (CCY-1a-E2) is a potent compound that acts against multiple human leukemia cell lines. CCY-1a-E2 was also shown to have efficacious anti‑leukemic activity in vivo. However, the molecular mechanism of action of CCY‑1a‑E2 attributed to its anticancer effect remains poorly understood. In the present study, CCY‑1a‑E2 suppressed cell viability in multiple leukemia cell lines (HL‑60, K562, KG‑1 and KG‑1a) via inhibition of cell proliferation, cell cycle arrest and induction of apoptosis. CCY‑1a‑E2 exhibited a marked toxic effect on HL‑60 cells and displayed low cytotoxicity in normal human peripheral blood mononuclear cells (PBMCs). Results from flow cytometric analysis indicated that CCY‑1a‑E2 promoted G2/M phase arrest and promoted apoptosis in the HL‑60 cells. CCY‑1a‑E2 treatment upregulated cyclin B, cyclin‑dependent kinase 1 (CDK1), cell division cycle 25C (cdc25C) and p21 protein expression. CCY‑1a‑E2 caused apoptotic cell death and DNA fragmentation as determined by 4',6‑diamidino‑2‑phenylindole (DAPI) staining and DNA gel electrophoresis. Elevated activities of caspase‑8, ‑9 and ‑3 were observed during CCY‑1a‑E2‑induced cell apoptosis; their specific inhibitors were found to block CCY‑1a‑E2‑induced apoptosis, respectively. Moreover, CCY‑1a‑E2 time‑dependently disrupted the mitochondrial membrane potential (ΔΨm), and it enhanced the protein levels of Fas/CD95, cytochrome c, Bax, cleaved PARP, as well as attenuated Bcl‑2 expression in the HL‑60 cells. Our results provide direct evidence that supports the future potential therapeutic application of CCY-1a-E2 in leukemia.

Introduction

Leukemia is characterized by uncontrolled cell proliferation and blockage in the differentiation of hematopoietic cells (1,2). However, clinical trials concerning treatment strategies for leukemia have not achieved satisfactory outcomes, and new targets for treating leukemia are necessary. One of the best strategies for new anti-leukemia agents are carried out via induction of cell differentiation or apoptotic death in leukemia cells (35). Regulation and/or management of cell cycle progression and apoptosis are prominent approaches to anti-leukemia therapy (2,6). Cyclin-dependent kinase (CDK) complexes can modulate cell cycle progression, especially cyclin-dependent kinase 1 (CDK1) and cyclin B are pivotal molecules in the regulation of the cell cycle in the G2/M phase (7,8). The cell division cycle 25C (cdc25C) phosphatase controls CDK1 activity and accelerates mitosis entry by dephosphorylation of CDK1 on Thr14 and Tyr15 sites (9,10). Additionally, the activity of CDK1/cyclin B complex is blocked by p21waf/cip1 signaling which serves as a CDK inhibitor (11,12). Several agents have been shown to interfere with the activity of CDK1 and cause subsequent cell cycle arrest, and these agents have been developed into significant clinical anticancer drugs through induction of cancer cell apoptosis (13,14). When tumor cells undergo apoptosis, nuclear condensation, DNA fragmentation and apoptotic bodies are manifested (15,16). During the apoptotic process, caspase proteins undergo proteolytic processing and trigger a cascade of caspase activation (17,18). Therefore, these key factors can regulate the apoptotic process and play a vital role in the treatment of leukemia.

Benzyloxybenzaldehyde derivatives have been reported to have multiple biological functions, including binding to estrogen receptors (ERα and β), arresting cell cycle progression and inducing apoptotic cell death (1921). A class of 2-benzyloxybenzaldehyde derivatives was designed and synthesized in our laboratory (20). 2-Benzyloxybenzaldehyde analog 2-[(3-methoxybenzyl)oxy]benzaldehyde (CCY-1a-E2) has been found to be a potent compound against leukemia cells. CCY-1a-E2 exhibited an anti-leukemic effect on a leukemia BALB/c mouse model (19). High dosage treatment (100 mg/kg) of CCY-1a-E2 was found to have no adverse effects on renal, hepatic and hematological parameters upon safety evaluation analysis (19). However, the molecular mechanism underlying the anti-leukemia effects of CCY-1a-E2 has not been completely clarified. Here, the anti-proliferative activity of CCY-1a-E2 was evaluated in human leukemia HL-60 cells. We found that CCY-1a-E2 led to cell cycle arrest at the G2/M phase and caused apoptosis in HL-60 cells through mitochondria-dependent caspase cascade signaling.

Materials and methods

Chemicals and reagents

Fetal bovine serum (FBS), L-glutamine, penicillin-streptomycin, RPMI-1640 medium, and trypsin-EDTA were obtained from Thermo Fisher Scientific, Inc. (Waltham, MA, USA). The primary antibodies used in this study and their corresponding IgG antibodies conjugated to horseradish peroxidase (HRP) were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Z-IETD-FMK (a specific caspase-8 inhibitor), Z-LEHD-FMK (a specific caspase-9 inhibitor) and Z-DEVD-FMK (a specific caspase-3 inhibitor) were obtained from R&D Systems, Inc. (Minneapolis, MN, USA). All chemicals and reagents were of analytical grade and purchased from Sigma-Aldrich Corp. (St. Louis, MO, USA) unless otherwise stated.

Cell culture

Human promyelocytic leukemia cell line HL-60 and human acute myelogenous leukemia cell lines KG-1 and KG-1a were purchased from the Bioresource Collection and Research Center (BCRC) (Hsinchu, Taiwan). K562 erythroleukemia cell line was purchased from the American Type Culture Collection (ATCC) (Manassas, VA, USA). Peripheral blood mononuclear cells (PBMCs) were collected from whole blood samples with the BD Vacutainer Mononuclear Cell Preparation Tube (CPT) with sodium heparin (Becton, Dickinson and Co., Franklin Lakes, NJ, USA) and were isolated using Ficoll-Paque™ Plus (GE Healthcare UK, Ltd., Little Chalfont, Buckinghamshire, UK). Cells were placed into 75-cm2 culture flasks and were grown in RPMI-1640 medium supplemented with 10% FBS, 100 U/ml penicillin, and 100 µg/ml streptomycin at 37°C under a humidified atmosphere of 5% CO2 and 95% air.

Detection of cell number and viability

HL-60, K562, KG-1 and KG-1a cells (1×104 cells/well) in 96-well plates were incubated with 0, 1, 2.5, 5 and 10 µM of CCY-1a-E2 for 24 and 48 h. The trypan blue dye exclusion assay was applied to determine the number of viable cells by using a Countess Automated Cell Counter (Thermo Fisher Scientific, Inc.) as previously reported (22). HL-60 cells were exposed to 0, 1, 2.5, 5 and 10 µM of CCY-1a-E2 after pre-incubation with or without 10, 25 and 50 µM of Z-IETD-FMK, Z-LEHD-FMK and Z-DEVD-FMK for 2 h. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed for the quantitative analysis of cell viability as described elsewhere (23,24).

Assessment of cell cycle distribution by flow cytometric analysis

HL-60 cells (2×105 cells/well) were seeded into 12-well plates and then treated with 5 µM CCY-1a-E2 for 0, 3, 6, 12 and 24 h. The cells were fixed and stained with propidium iodide (PI) solution following a previously reported method (6). The sub-G1 peak (apoptotic population) and cell cycle profiling were determined using a BD FACSCalibur flow cytometer (BD Biosciences, San Jose, CA, USA), and the data were analyzed by utilizing BD CellQuest software.

Immunoblotting analysis

HL-60 cells (1×107 cells) were placed in T75 flasks and treated with 5 µM CCY-1a-E2 for 0, 1, 2, 4, 6, 8, 10, 12 and 16 h. After treatments, the cells were lysed with lysis buffer, and each sample was electrophoresed as previously detailed (15,22,25), and the membrane was probed with an appropriate secondary antibody for enhanced chemiluminescence (Immobilon Western HRP Substrate; Merck Millipore, Bedford, MA, USA).

4′,6-Diamidino-2-phenylindole (DAPI) staining and DNA fragmentation assay

HL-60 cells (2×105 cells/ml) were treated with 5 µM CCY-1a-E2 for 24 h and thereafter stained with 1 µg/ml DAPI as previously described (16,25). After a 48-h exposure, DNA was extracted from each sample and electrophoresis was run according to a previous method (26).

Analyses of caspase-3, -8 and -9 activities

HL-60 cells at a density of 1×107 cells/flask were incubated with 5 µM CCY-1a-E2 for 0, 2, 4, 8 and 12 h. At the end of the incubation, the cells were lysed and assessed according to the manufacturer's instructions provided in the Caspase-3, -8 and -9 Colorimetric Assay kits (R&D Systems, Inc.).

Determination of mitochondrial membrane potential (ΔΨm)

HL-60 cells at a density of 2×105 cells/well in 12-well plates were treated with 5 µM CCY-1a-E2 for 0, 6, 12, 18 and 24 h. Cells from each treatment were harvested and re-suspended in 500 µl of DiOC6 (3) (Thermo Fisher Scientific, Inc.) at 50 nM for ΔΨm. After incubation at 37°C for 30 min, the cells were analyzed by flow cytometry as described by Lee et al (27).

Statistical analysis

The statistical significance of the difference was defined (p<0.05) and carried out utilizing Student's t-test, and the data are expressed as the mean ± standard deviation (SD) from three independent experiments.

Results

CCY-1a-E2 reduces cell viability in leukemia cells

Four cell lines (HL-60, K562, KG-1 and KG-1a) were used to assess the cytotoxicity of CCY-1a-E2. The cells were treated with 0, 1, 2.5, 5 and 10 µM of CCY-1a-E2 for 24 h, and the viable cell number was measured by trypan blue dye exclusion assay. CCY-1a-E2 dose-dependently decreased the viability of the HL-60, K562, KG-1 and KG-1a cells. Notaby, HL-60 cells were more sensitive to CCY-1a-E2 than the three other cell lines (Fig. 1A). In addition, the inhibitory effect of CCY-1a-E2 on the proliferation of HL-60 cells was time-dependent (Fig. 1B), and the half maximal inhibitory concentration (IC50) value for the 48-h treatment of CCY-1a-E2 in the HL-60 cell line was 5.32±0.25 µM. In contrast, CCY-1a-E2 exerted low cytotoxicity against normal human PBMCs (Fig. 2). Our results suggest that CCY-1a-E2 exhibits anti-leukemia action against HL-60 cells in vitro.

CCY-1a-E2 causes G2/M phase arrest in HL-60 cells

To explore the effect of CCY-1a-E2 on cell cycle distribution, the cells were treated for various time periods with CCY-1a-E2. The percentage of treated cells in phase G1, S and G2/M was detected by DNA content stained with PI. CCY-1a-E2 induced G2/M phase arrest and increased the sub-G1 population (apoptotic cells) (Fig. 3A). Exposure of HL-60 cells to 5 µM CCY-1a-E2 for 6, 12 and 24 h resulted in a significant increase in the percentage of cells in the G2/M phase, while a marked decrease in the percentage of cells in the G0/G1 phase was observed (Fig. 3B). To examine the protein levels associated with the G2/M phase, CCY-1a-E2-treated cells were analyzed by immunoblotting. The protein expression levels of cyclin B, CDK1, cdc25C were increased. However, after 6 hours of treatment, cdc25C protein level gradually decreased, but p21 protein level significantly increased (Fig. 4). These findings suggest that CCY-1a-E2 regulated CDK1 activation and resulted in G2/M phase arrest in the HL-60 cells.

CCY-1a-E2 triggers apoptotic death in HL-60 cells

In Fig. 3, the percentage of cells in the sub-G1 phase (apoptotic cells) was increased after CCY-1a-E2 exposure. To evaluate whether CCY-1a-E2 induces apoptosis, we performed DAPI staining for chromatin condensation and a DNA fragmentation assay. The number of cells with brighter cell nuclei were elevated in the CCY-1a-E2-treated cells (Fig. 5A). In addition, DNA was extracted from the cells following treatment with various concentrations of CCY-1a-E2 for 24 h, and subjected to agarose gel electrophoresis. DNA ladder was observed in samples treated with 5, 10 and 15 µM CCY-1a-E2 (Fig. 5B). Our results imply that CCY-1a-E2 treatment induced apoptosis in the HL-60 cells.

CCY-1a-E2 enhances caspase-9, -8 and -3 activities in HL-60 cells

To further ascertain whether CCY-1a-E2-induced apoptosis is caspase-dependent, we performed specific caspase activity assays. HL-60 cells were exposed to 5 µM CCY-1a-E2 for different time periods. We found that CCY-1a-E2 enhanced the activities of caspase-9, -3 and -8 (Fig. 6A) after 2, 4, 8 and 12 h of exposure. To confirm the role of caspase-mediated apoptosis by CCY-1a-E2, the cells were pre-treated with specific caspase inhibitors. Our data showed that Z-IETD-FMK, Z-LEHD-FMK and Z-DEVE-FMK at 10, 25 and 50 µM concentration-dependently suppressed CCY-1a-E2-reduced cell viability (Fig. 6B). These data indicated that CCY-1a-E2-induced apoptosis was mediated via both extrinsic and intrinsic signaling in the HL-60 cells.

CCY-1a-E2 collapses ΔΨm and alters mitochondria-mediated apoptosis signaling in HL-60 cells

To confirm whether the CCY-1a-E2-provoked apoptosis was mediated via the mitochondrial pathway, the level of ΔΨm was measured and immunoblotting was carried out. CCY-1a-E2 treatment led to a decrease in ΔΨm in a time-course pattern (Fig. 7). CCY-1a-E2 also promoted the protein expression of cytochrome c, Bax and PARP, while it suppressed the level of Bcl-2 (Fig. 8). Additionally, activation of Fas/CD95 protein occurred in the treated cells (Fig. 8). Therefore, these results conclude that CCY-1a-E2-mediated apoptosis of HL-60 cells was carried out through the mitochondria- and death receptor-dependent pathway.

Discussion

2-Benzyloxybenzaldehyde (CCY-1a) has been shown to inhibit superoxide anion generation via Akt inactivation and phospholipase D activation in rat neutrophils (21). In addition, 2-benzyloxybenzaldehyde can block Ca2+ entry and suppress formyl peptide-stimulated increase in intracellular Ca2+ in neutrophils (28). Pan et al (29) demonstrated that 2-benzy-loxybenzaldehyde modulates vascular smooth muscle cell proliferation by blocking the Ras/p42/44 MAPK pathway and inhibiting NF-κB and AP-1 DNA binding activities. Our previous study showed that CCY-1a-E2 reduced the percentage of viable murine leukemia WEHI-3 cells, and the IC50 value of CCY-1a-E2 was 5 µM for a 24-h treatment (19). To evaluate the anticancer effect of CCY-1a-E2 on other leukemia cell lines, we treated HL-60, K562, KG-1 and KG-1a cells with various concentrations of CCY-1a-E2. A significant concentration-dependent decrease of cell viability was observed in all cell lines after 24 h exposure to 2.5-10 µM of CCY-1a-E2. CCY-1a-E2 not only decreased the cell viability in HL-60 cells, but also exerted low cytotoxicity in PBMCs (Figs. 1 and 2). The clinical applicability of an anti-leukemia drug depends on its being distinct in both its potency and therapeutic index between leukemia and normal blood cells (6). Our result was in agreement with the previous study of CCY-1a-E2 on reducing viability of WEHI-3 cells (19). Overall, CCY-1a-E2 represents a promising candidate as an anticancer agent for leukemia due to its low toxicity to normal cells.

CCY-1a-E2 significantly inhibited cell viability and induced cell apoptosis in the HL-60 cells (Figs. 1 and 3). However, the molecular mechanisms of its anti-leukemia activity remain unknown. Our data demonstrated that CCY-1a-E2 triggered cell cycle arrest at the G2/M phase after a 6-h exposure, while its effect on the sub-G1 cell population appeared following a 12-h treatment (Fig. 3A). This result indicated that CCY-1a-E2-induced G2/M phase arrest occurred before the onset of apoptosis. The CDK1/cyclin B complex is one of the main regulators, resulting in G2/M progression and apoptosis. G2/M checkpoints can be exerted by inactivating cdc25C and activating CDK inhibitor (p21waf/cip), which subsequently inactivates CDK1, and prevents cells from entering mitosis (9,12). In the present study, we investigated the expression levels of G2/M phase-related proteins. Our results demonstrated that CCY-1a-E2 induced the expression of cyclin B, CDK1, cdc25C and p21 in a time-dependent manner (Fig. 4). Our finding implicates a novel role for the 2-benzyloxybenzaldehyde derivative.

Cells undergo apoptosis in response to cell death-inducing signals from death receptors on the cell surface [such as Fas, death receptor 4 (DR4) or DR5], mitochondria, or endoplasmic reticulum (ER) stress (3032). During apoptosis, caspases are activated and arranged in a proteolytic cascade to convey the apoptotic signal (17,18). Apoptotic evidence of chromatin condensation and DNA fragmentation was observed in the CCY-1a-E2-treated HL-60 cells, indicating that cells underwent apoptosis in response to CCY-1a-E2 treatment (Fig. 5). Moreover, induction of caspase-9, -8 and -3 activities (Fig. 6) as well as the specific cleavage of PARP (Fig. 8) were detected in the CCY-1a-E2-treated cells. Application of the intrinsic and extrinsic caspase specific inhibitors blocked the CCY-1a-E2-reduced cell viability (Fig. 6). This result further confirmed the effect of CCY-1a-E2 on apoptotic pathways. The caspase-independent factors such as apoptosis-inducing factor (AIF) and endonuclease G (Endo G) can be released from mitochondria into the cytosol (33,34). Our results fail to exclude the possibility of a caspase-independent pathway involved in the CCY-1a-E2-induced apoptosis.

Loss of ΔΨm is one of the features of apoptosis (35,36). Indeed, we detected a significant loss of ΔΨm in the CCY-1a-E2-treated HL-60 cells (Fig. 7). Moreover, cytochrome c release is related to the change in Bcl-2 family proteins during cell apoptosis. Bcl-2 and Bax are located in the mitochondrial outer membrane, and their ratio (Bcl-2/Bax) regulates the release of apoptotic elements (cytochrome c, pro-caspase-9, AIF and Endo G) to the cytosol (18,31,35). Our data indicated that the protein expression levels of cytochrome c, Bax and PARP (p85) were increased, whereas the protein expression level of Bcl-2 was decreased in the CCY-1a-E2-treated HL-60 cells (Fig. 8). Our results are consistent with these available observations as evidenced by the upregulation of Bax protein and the downregulation of Bcl-2 protein in the CCY-1a-E2-treated cells. These results suggest that the activation of the caspase cascade contributed to CCY-1a-E2-induced apoptosis of HL-60 cells.

In conclusion, we demonstrated that CCY-1a-E2 exerts cytotoxic activity against leukemia cells, while it is less toxic to normal PBMCs. CCY-1a-E2 induced G2/M phase arrest followed by caspase-mediated apoptosis in the human leukemia HL-60 cells. Taken together, these findings provide important new insights into the possible molecular mechanisms of the anti-leukemia activity of CCY-1a-E2.

References

1 

Riether C, Schürch CM and Ochsenbein AF: Regulation of hematopoietic and leukemic stem cells by the immune system. Cell Death Differ. 22:187–198. 2015. View Article : Google Scholar :

2 

Lu CC, Yang JS, Chiang JH, Hour MJ, Lin KL, Lin JJ, Huang WW, Tsuzuki M, Lee TH and Chung JG: Novel quinazolinone MJ-29 triggers endoplasmic reticulum stress and intrinsic apoptosis in murine leukemia WEHI-3 cells and inhibits leukemic mice. PLoS One. 7:e368312012. View Article : Google Scholar : PubMed/NCBI

3 

Wang W, Lv M, Zhao X and Zhang J: Developing a novel indolocarbazole as histone deacetylases inhibitor against leukemia cell lines. J Anal Methods Chem. 2015:6750532015. View Article : Google Scholar : PubMed/NCBI

4 

Maioral MF, Gaspar PC, Rosa Souza GR, Mascarello A, Chiaradia LD, Licínio MA, Moraes AC, Yunes RA, Nunes RJ and Santos-Silva MC: Apoptotic events induced by synthetic naphthylchalcones in human acute leukemia cell lines. Biochimie. 95:866–874. 2013. View Article : Google Scholar

5 

Padma VV: An overview of targeted cancer therapy. Biomedicine (Taipei). 5:192015. View Article : Google Scholar

6 

Yang JS, Hour MJ, Huang WW, Lin KL, Kuo SC and Chung JG: MJ-29 inhibits tubulin polymerization, induces mitotic arrest, and triggers apoptosis via cyclin-dependent kinase 1-mediated Bcl-2 phosphorylation in human leukemia U937 cells. J Pharmacol Exp Ther. 334:477–488. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Kan SF, Huang WJ, Lin LC and Wang PS: Inhibitory effects of evodiamine on the growth of human prostate cancer cell line LNCaP. Int J Cancer. 110:641–651. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Murray AW: Recycling the cell cycle: Cyclins revisited. Cell. 116:221–234. 2004. View Article : Google Scholar : PubMed/NCBI

9 

Timofeev O, Cizmecioglu O, Settele F, Kempf T and Hoffmann I: Cdc25 phosphatases are required for timely assembly of CDK1-cyclin B at the G2/M transition. J Biol Chem. 285:16978–16990. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Castedo M, Perfettini JL, Roumier T and Kroemer G: Cyclin-dependent kinase-1: Linking apoptosis to cell cycle and mitotic catastrophe. Cell Death Differ. 9:1287–1293. 2002. View Article : Google Scholar : PubMed/NCBI

11 

Yang JS, Hour MJ, Kuo SC, Huang LJ and Lee MR: Selective induction of G2/M arrest and apoptosis in HL-60 by a potent anticancer agent, HMJ-38. Anticancer Res. 24:1769–1778. 2004.PubMed/NCBI

12 

Elsayed YA and Sausville EA: Selected novel anticancer treatments targeting cell signaling proteins. Oncologist. 6:517–537. 2001. View Article : Google Scholar : PubMed/NCBI

13 

Shah MA and Schwartz GK: Cell cycle-mediated drug resistance: An emerging concept in cancer therapy. Clin Cancer Res. 7:2168–2181. 2001.PubMed/NCBI

14 

Chan KS, Koh CG and Li HY: Mitosis-targeted anti-cancer therapies: Where they stand. Cell Death Dis. 3:e4112012. View Article : Google Scholar : PubMed/NCBI

15 

Lu CC, Yang JS, Chiang JH, Hour MJ, Lin KL, Lee TH and Chung JG: Cell death caused by quinazolinone HMJ-38 challenge in oral carcinoma CAL 27 cells: Dissections of endoplasmic reticulum stress, mitochondrial dysfunction and tumor xenografts. Biochim Biophys Acta. 1840:2310–2320. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Chiang JH, Yang JS, Lu CC, Hour MJ, Chang SJ, Lee TH and Chung JG: Newly synthesized quinazolinone HMJ-38 suppresses angiogenetic responses and triggers human umbilical vein endothelial cell apoptosis through p53-modulated Fas/death receptor signaling. Toxicol Appl Pharmacol. 269:150–162. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Chung JG, Yang JS, Huang LJ, Lee FY, Teng CM, Tsai SC, Lin KL, Wang SF and Kuo SC: Proteomic approach to studying the cytotoxicity of YC-1 on U937 leukemia cells and antileukemia activity in orthotopic model of leukemia mice. Proteomics. 7:3305–3317. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Sanjiv K, Su TL, Suman S, Kakadiya R, Lai TC, Wang HY, Hsiao M and Lee TC: The novel DNA alkylating agent BO-1090 suppresses the growth of human oral cavity cancer in xenografted and orthotopic mouse models. Int J Cancer. 130:1440–1450. 2012. View Article : Google Scholar

19 

Lin C, Yang JS, Tsai SC, Lin CF and Lee MR: In vivo evaluation of the synthesized novel 2-benzyloxybenzaldehyde analog CCY-1a-E2 for the treatment of leukemia in the BALB/c mouse WEHI-3 allograft model. Oncol Lett. 5:777–782. 2013.PubMed/NCBI

20 

Chang C, Kuo S, Lin Y, Wang J and Huang L: Benzyloxybenzaldehyde analogues as novel adenylyl cyclase activators. Bioorg Med Chem Lett. 11:1971–1974. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Wang JP, Chang LC, Hsu MF, Huang LJ and Kuo SC: 2-Benzyloxy-benzaldehyde inhibits formyl-methionyl-leucyl-phenylalanine stimulation of phospholipase D activation in rat neutrophils. Biochim Biophys Acta. 1573:26–32. 2002. View Article : Google Scholar : PubMed/NCBI

22 

Liao CL, Lai KC, Huang AC, Yang JS, Lin JJ, Wu SH, Gibson Wood W, Lin JG and Chung JG: Gallic acid inhibits migration and invasion in human osteosarcoma U-2 OS cells through suppressing the matrix metalloproteinase-2/-9, protein kinase B (PKB) and PKC signaling pathways. Food Chem Toxicol. 50:1734–1740. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Kao TC, Shyu MH and Yen GC: Neuroprotective effects of glycyrrhizic acid and 18beta-glycyrrhetinic acid in PC12 cells via modulation of the PI3K/Akt pathway. J Agric Food Chem. 57:754–761. 2009. View Article : Google Scholar

24 

Lu CC, Yang SH, Hsia SM, Wu CH and Yen GC: Inhibitory effects of Phyllanthus emblica L. on hepatic steatosis and liver fibrosis in vitro. J Funct Foods. 20:20–30. 2016. View Article : Google Scholar

25 

Chang PY, Peng SF, Lee CY, Lu CC, Tsai SC, Shieh TM, Wu TS, Tu MG, Chen MY and Yang JS: Curcumin-loaded nanoparticles induce apoptotic cell death through regulation of the function of MDR1 and reactive oxygen species in cisplatin-resistant CAR human oral cancer cells. Int J Oncol. 43:1141–1150. 2013.PubMed/NCBI

26 

Lin CC, Chuang YJ, Yu CC, Yang JS, Lu CC, Chiang JH, Lin JP, Tang NY, Huang AC and Chung JG: Apigenin induces apoptosis through mitochondrial dysfunction in U-2 OS human osteosarcoma cells and inhibits osteosarcoma xenograft tumor growth in vivo. J Agric Food Chem. 60:11395–11402. 2012. View Article : Google Scholar : PubMed/NCBI

27 

Lee CY, Chien YS, Chiu TH, Huang WW, Lu CC, Chiang JH and Yang JS: Apoptosis triggered by vitexin in U937 human leukemia cells via a mitochondrial signaling pathway. Oncol Rep. 28:1883–1888. 2012.PubMed/NCBI

28 

Wang JP, Chang LC, Kuan YH, Tsao LT, Huang LJ and Kuo SC: 2-Benzyloxybenzaldehyde inhibits formyl peptide-stimulated increase in intracellular Ca2+ in neutrophils mainly by blocking Ca2+ entry. Naunyn Schmiedebergs Arch Pharmacol. 370:353–360. 2004. View Article : Google Scholar : PubMed/NCBI

29 

Pan SL, Guh JH, Huang YW, Chang YL, Chang CY, Huang LJ, Kuo SC and Teng CM: Inhibition of Ras-mediated cell proliferation by benzyloxybenzaldehyde. J Biomed Sci. 9:622–630. 2002. View Article : Google Scholar : PubMed/NCBI

30 

Lavrik IN, Golks A and Krammer PH: Caspases: Pharmacological manipulation of cell death. J Clin Invest. 115:2665–2672. 2005. View Article : Google Scholar : PubMed/NCBI

31 

Orrenius S: Reactive oxygen species in mitochondria-mediated cell death. Drug Metab Rev. 39:443–455. 2007. View Article : Google Scholar : PubMed/NCBI

32 

Ho TF and Chang CC: A promising 'TRAIL' of tanshinones for cancer therapy. Biomedicine (Taipei). 5:232015. View Article : Google Scholar

33 

Kuo HM, Tsai HC, Lin YL, Yang JS, Huang AC, Yang MD, Hsu SC, Chung MC, Gibson Wood W and Chung JG: Mitochondrial-dependent caspase activation pathway is involved in baicalein-induced apoptosis in human hepatoma J5 cells. Int J Oncol. 35:717–724. 2009.PubMed/NCBI

34 

Strauss G, Westhoff MA, Fischer-Posovszky P, Fulda S, Schanbacher M, Eckhoff SM, Stahnke K, Vahsen N, Kroemer G and Debatin KM: 4-Hydroperoxy-cyclophosphamide mediates caspase-independent T-cell apoptosis involving oxidative stress-induced nuclear relocation of mitochondrial apoptogenic factors AIF and EndoG. Cell Death Differ. 15:332–343. 2008. View Article : Google Scholar

35 

Tsai SC, Huang WW, Huang WC, Lu CC, Chiang JH, Peng SF, Chung JG, Lin YH, Hsu YM, Amagaya S, et al: ERK-modulated intrinsic signaling and G(2)/M phase arrest contribute to the induction of apoptotic death by allyl isothiocyanate in MDA-MB-468 human breast adenocarcinoma cells. Int J Oncol. 41:2065–2072. 2012.PubMed/NCBI

36 

Huang TT, Lin HC, Chen CC, Lu CC, Wei CF, Wu TS, Liu FG and Lai HC: Resveratrol induces apoptosis of human nasopharyngeal carcinoma cells via activation of multiple apoptotic pathways. J Cell Physiol. 226:720–728. 2011. View Article : Google Scholar

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September-2016
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Spandidos Publications style
Lin CF, Yang JS, Lin C, Tsai FJ, Lu CC and Lee MR: CCY-1a-E2 induces G2/M phase arrest and apoptotic cell death in HL-60 leukemia cells through cyclin-dependent kinase 1 signaling and the mitochondria-dependent caspase pathway. Oncol Rep 36: 1633-1639, 2016.
APA
Lin, C., Yang, J., Lin, C., Tsai, F., Lu, C., & Lee, M. (2016). CCY-1a-E2 induces G2/M phase arrest and apoptotic cell death in HL-60 leukemia cells through cyclin-dependent kinase 1 signaling and the mitochondria-dependent caspase pathway. Oncology Reports, 36, 1633-1639. https://doi.org/10.3892/or.2016.4970
MLA
Lin, C., Yang, J., Lin, C., Tsai, F., Lu, C., Lee, M."CCY-1a-E2 induces G2/M phase arrest and apoptotic cell death in HL-60 leukemia cells through cyclin-dependent kinase 1 signaling and the mitochondria-dependent caspase pathway". Oncology Reports 36.3 (2016): 1633-1639.
Chicago
Lin, C., Yang, J., Lin, C., Tsai, F., Lu, C., Lee, M."CCY-1a-E2 induces G2/M phase arrest and apoptotic cell death in HL-60 leukemia cells through cyclin-dependent kinase 1 signaling and the mitochondria-dependent caspase pathway". Oncology Reports 36, no. 3 (2016): 1633-1639. https://doi.org/10.3892/or.2016.4970