Glucose-regulated protein 78 contributes to the proliferation and tumorigenesis of human colorectal carcinoma via AKT and ERK pathways

  • Authors:
    • Xuan Zhou
    • Xiaoming Xing
    • Shuping Zhang
    • Lili Liu
    • Chengqin Wang
    • Lin Li
    • Qiuxia Ji
    • Huamin Liu
  • View Affiliations

  • Published online on: September 16, 2016     https://doi.org/10.3892/or.2016.5097
  • Pages: 2723-2730
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Abstract

Glucose-regulated protein 78 (GRP78), a molecular chaperon in the endoplasmic reticulum (ER), is overexpressed in a variety of tumor types and plays a critical role in cancer cell proliferation, migration, invasion and drug resistance. However, the mechanisms underlying the role of GRP78 in tumor carcinogenesis remain largely unknown. In the present study, we found that GRP78 knockdown in colorectal carcinoma (CRC) cells significantly inhibited cell proliferation, colony formation and tumorigenesis in vitro and in vivo. The proliferation inhibition of CRC cells by GRP78 knockdown was associated with an S phase arrest, a reduced G1/S transition, and a downregulation of phosphorylation of AKT and ERK1/2, key cell cycle regulatory proteins. In addition, GRP78 knockdown enhanced the apoptosis induced by 5-fluorouracil (5-FU) in CRC cells. Taken together, our results indicate that GRP78 plays an important role in the development and progression of CRC and may have therapeutic potential for CRC patients.
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November-2016
Volume 36 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Zhou X, Xing X, Zhang S, Liu L, Wang C, Li L, Ji Q and Liu H: Glucose-regulated protein 78 contributes to the proliferation and tumorigenesis of human colorectal carcinoma via AKT and ERK pathways. Oncol Rep 36: 2723-2730, 2016.
APA
Zhou, X., Xing, X., Zhang, S., Liu, L., Wang, C., Li, L. ... Liu, H. (2016). Glucose-regulated protein 78 contributes to the proliferation and tumorigenesis of human colorectal carcinoma via AKT and ERK pathways. Oncology Reports, 36, 2723-2730. https://doi.org/10.3892/or.2016.5097
MLA
Zhou, X., Xing, X., Zhang, S., Liu, L., Wang, C., Li, L., Ji, Q., Liu, H."Glucose-regulated protein 78 contributes to the proliferation and tumorigenesis of human colorectal carcinoma via AKT and ERK pathways". Oncology Reports 36.5 (2016): 2723-2730.
Chicago
Zhou, X., Xing, X., Zhang, S., Liu, L., Wang, C., Li, L., Ji, Q., Liu, H."Glucose-regulated protein 78 contributes to the proliferation and tumorigenesis of human colorectal carcinoma via AKT and ERK pathways". Oncology Reports 36, no. 5 (2016): 2723-2730. https://doi.org/10.3892/or.2016.5097