MicroRNA-361-5p inhibits epithelial-to-mesenchymal transition of glioma cells through targeting Twist1

  • Authors:
    • Xi Zhang
    • Chunyan Wei
    • Jin Li
    • Jiali Liu
    • Jianqiang Qu
  • View Affiliations

  • Published online on: January 25, 2017     https://doi.org/10.3892/or.2017.5406
  • Pages: 1849-1856
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Abstract

MicroRNA-361-5p (miR-361-5p) has been reported to be dysregulated in various human cancer types. However, the function of miR-361-5p in glioma remains unknown. In the present study, we aimed to investigate the biological functions of miR-361-5p in regulating glioma progression and the underlying molecular mechanism. We found that miR-361-5p was significantly decreased in glioma tissues and cell lines as detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis. Functional analysis revealed that miR-361-5p overexpression significantly inhibited glioma cell migration, invasion and epithelial-mesenchymal transition (EMT) whereas suppression of miR-361-5p showed opposite effects. Bioinformatic analysis showed that Twist1, a critical EMT inducer, was a predicted target of miR-361-5p which was validated by dual-luciferase reporter assay, RT-qPCR and western blot analysis. Further analysis indicated that miR-361-5p regulates the Twist1/Bmi-1 signaling axis. Rescue experiments showed that restoration of Twist1 expression significantly reversed the suppressive effect of miR-361-5p on cell migration, invasion and EMT. Taken together, the present study demonstrated an important role of miR-361-5p in glioma - which regulated the EMT of glioma cells by targeting and regulating Twist1. These findings provide novel insight into understanding the role and mechanism of miR-361-5p in regulating the biolo­gical behavior of glioma cells and suggest that miR-361-5p is a novel potential therapeutic target for glioma.
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March-2017
Volume 37 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Zhang X, Wei C, Li J, Liu J and Qu J: MicroRNA-361-5p inhibits epithelial-to-mesenchymal transition of glioma cells through targeting Twist1. Oncol Rep 37: 1849-1856, 2017.
APA
Zhang, X., Wei, C., Li, J., Liu, J., & Qu, J. (2017). MicroRNA-361-5p inhibits epithelial-to-mesenchymal transition of glioma cells through targeting Twist1. Oncology Reports, 37, 1849-1856. https://doi.org/10.3892/or.2017.5406
MLA
Zhang, X., Wei, C., Li, J., Liu, J., Qu, J."MicroRNA-361-5p inhibits epithelial-to-mesenchymal transition of glioma cells through targeting Twist1". Oncology Reports 37.3 (2017): 1849-1856.
Chicago
Zhang, X., Wei, C., Li, J., Liu, J., Qu, J."MicroRNA-361-5p inhibits epithelial-to-mesenchymal transition of glioma cells through targeting Twist1". Oncology Reports 37, no. 3 (2017): 1849-1856. https://doi.org/10.3892/or.2017.5406