The specific killing effect of matrine on castration-resistant prostate cancer cells by targeting the Akt/FoxO3a signaling pathway Corrigendum in /10.3892/or.2020.7784

Bai,Shoumin; Chen,Ting; Yu,Xiaoli; Luo,Ming; Chen,Xianju; Lin,Chunhao; Lai,Yiming; Huang,Hai

doi:10.3892/or.2017.5510

The specific killing effect of matrine on castration-resistant prostate cancer cells by targeting the Akt/FoxO3a signaling pathway

Corrigendum in 10.3892/or.2020.7784

Authors:
- Shoumin Bai
- Ting Chen
- Xiaoli Yu
- Ming Luo
- Xianju Chen
- Chunhao Lin
- Yiming Lai
- Hai Huang
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Affiliations: Department of Radiation Oncology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, P.R. China, Department of Urology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, P.R. China
Published online on: March 16, 2017 https://doi.org/10.3892/or.2017.5510
Pages: 2819-2828

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Abstract

Matrine, a Sophora alkaloid, exhibits antiproliferative and anti-carcinogenic activities through several mechanisms. In a previous study, we found that matrine could effectively inhibit the proliferation of castration-resistant prostate cancer (CRPC). In the present study, the effect of matrine and LY294002 on the expression of the Akt/FoxO3a signaling pathway was examined by western blot analyses and RT-PCR. We discovered that matrine significantly inhibited the proliferation of both prostate cancer cell line PC-3 and prostate epithelial cell line RWPE1, induced apoptosis and induced cell cycle arrest. In addition, LY294002 was found to enhance the effect of matrine. Furthermore, the effects of matrine on the inhibition of proliferation and the induction of cell cycle arrest and cell apoptosis were more effective on PC-3 than on RWPE1 cells. Compared to RWPE1 cells, matrine exerted a more powerful influence on PC-3 cells in increasing the expression of the relevant protein. Our data suggested that FoxO3a-Bim and FoxO3a-P27 may mediate matrine-inhibited proliferation of CRPC cells by activating cell apoptosis and inducing cell cycle arrest. Matrine exhibited high selectivity in killing CRPC cells. Our findings demonstrated that matrine could be used in a potential therapeutic role in the management of CRPC in humans.

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1	Siegel RL, Miller KD and Jemal A: Cancer statistics, 2015. CA Cancer J Clin. 65:5–29. 2015. View Article : Google Scholar : PubMed/NCBI
2	Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J and Jemal A: Global cancer statistics, 2012. CA Cancer J Clin. 65:87–108. 2015. View Article : Google Scholar : PubMed/NCBI
3	Fowke JH, McLerran DF, Gupta PC, He J, Shu XO, Ramadas K, Tsugane S, Inoue M, Tamakoshi A, Koh WP, et al: Associations of body mass index, smoking, and alcohol consumption with prostate cancer mortality in the Asia Cohort Consortium. Am J Epidemiol. 182:381–389. 2015. View Article : Google Scholar : PubMed/NCBI
4	Sarker D, Reid AHM, Yap TA and de Bono JS: Targeting the PI3K/AKT pathway for the treatment of prostate cancer. Clin Cancer Res. 15:4799–4805. 2009. View Article : Google Scholar : PubMed/NCBI
5	Shukla S: FOXO3a: A potential target in prostate cancer. Austin J Urol. 12014.
6	Daitoku H, Sakamaki J and Fukamizu A: Regulation of FoxO transcription factors by acetylation and protein-protein interactions. Biochim Biophys Acta. 1813:1954–1960. 2011. View Article : Google Scholar : PubMed/NCBI
7	Arrendale A, Kim K, Choi JY, Li W, Geahlen RL and Borch RF: Synthesis of a phosphoserine mimetic prodrug with potent 14-3-3 protein inhibitory activity. Chem Biol. 19:764–771. 2012. View Article : Google Scholar : PubMed/NCBI
8	Rinner O, Mueller LN, Hubálek M, Müller M, Gstaiger M and Aebersold R: An integrated mass spectrometric and computational framework for the analysis of protein interaction networks. Nat Biotechnol. 25:345–352. 2007. View Article : Google Scholar : PubMed/NCBI
9	Wang W, You RL, Qin WJ, Hai LN, Fang MJ, Huang GH, Kang RX, Li MH, Qiao YF, Li JW, et al: Anti-tumor activities of active ingredients in Compound Kushen Injection. Acta Pharmacol Sin. 36:676–679. 2015. View Article : Google Scholar : PubMed/NCBI
10	Zhou Y, Wu Y, Deng L, Chen L, Zhao D, Lv L, Chen X, Man J, Wang Y, Shan H, et al: The alkaloid matrine of the root of Sophora flavescens prevents arrhythmogenic effect of ouabain. Phytomedicine. 21:931–935. 2014. View Article : Google Scholar : PubMed/NCBI
11	Guo B, Zhang T, Su J, Wang K and Li X: Oxymatrine targets EGFR^p-Tyr845 and inhibits EGFR-related signaling pathways to suppress the proliferation and invasion of gastric cancer cells. Cancer Chemother Pharmacol. 75:353–363. 2015. View Article : Google Scholar : PubMed/NCBI
12	Xie M, He G, Wang R, Shi S, Chen J, Ye Y, Xie L, Yi X and Tang A: Matrine-induced apoptosis of human nasopharyngeal carcinoma cells via in vitro vascular endothelial growth factor-A/extracellular signal-regulated kinase1/2 pathway inactivation. Horm Metab Res. 46:556–560. 2014. View Article : Google Scholar : PubMed/NCBI
13	Zhang L, Wang T, Wen X, Wei Y, Peng X, Li H and Wei L: Effect of matrine on HeLa cell adhesion and migration. Eur J Pharmacol. 563:69–76. 2007. View Article : Google Scholar : PubMed/NCBI
14	Liu Y, Xu Y, Ji W, Li X, Sun B, Gao Q and Su C: Anti-tumor activities of matrine and oxymatrine: Literature review. Tumour Biol. 35:5111–5119. 2014. View Article : Google Scholar : PubMed/NCBI
15	Shukla S, Bhaskaran N, Babcook MA, Fu P, Maclennan GT and Gupta S: Apigenin inhibits prostate cancer progression in TRAMP mice via targeting PI3K/Akt/FoxO pathway. Carcinogenesis. 35:452–460. 2014. View Article : Google Scholar : PubMed/NCBI
16	Li Q, Lai Y, Wang C, Xu G, He Z, Shang X, Sun Y, Zhang F, Liu L and Huang H: Matrine inhibits the proliferation, invasion and migration of castration-resistant prostate cancer cells through regulation of the NF-κB signaling pathway. Oncol Rep. 35:375–381. 2016.PubMed/NCBI
17	Wang D, Yang Y, Huang R, Zhang Z and Lin X: Myostatin activates the ubiquitin-proteasome and autophagy-lysosome systems contributing to muscle wasting in chronic kidney disease. Oxid Med Cell Longev. 2015:6849652015. View Article : Google Scholar : PubMed/NCBI
18	Majumder PK and Sellers WR: Akt-regulated pathways in prostate cancer. Oncogene. 24:7465–7474. 2005. View Article : Google Scholar : PubMed/NCBI
19	Carnero A: The PKB/AKT pathway in cancer. Curr Pharm Des. 16:34–44. 2010. View Article : Google Scholar : PubMed/NCBI
20	Trotman LC, Alimonti A, Scaglioni PP, Koutcher JA, Cordon-Cardo C and Pandolfi PP: Identification of a tumour suppressor network opposing nuclear Akt function. Nature. 441:523–527. 2006. View Article : Google Scholar : PubMed/NCBI
21	Zhang P, Wang Z, Chong T and Ji Z: Matrine inhibits proliferation and induces apoptosis of the androgen-independent prostate cancer cell line PC-3. Mol Med Rep. 5:783–787. 2012.PubMed/NCBI
22	Yaoxian W, Hui Y, Yunyan Z, Yanqin L, Xin G and Xiaoke W: Emodin induces apoptosis of human cervical cancer hela cells via intrinsic mitochondrial and extrinsic death receptor pathway. Cancer Cell Int. 13:712013. View Article : Google Scholar : PubMed/NCBI
23	Nam YJ, Mani K, Ashton AW, Peng CF, Krishnamurthy B, Hayakawa Y, Lee P, Korsmeyer SJ and Kitsis RN: Inhibition of both the extrinsic and intrinsic death pathways through nonhomotypic death-fold interactions. Mol Cell. 15:901–912. 2004. View Article : Google Scholar : PubMed/NCBI
24	Heath-Engel HM and Shore GC: Regulated targeting of Bax and Bak to intracellular membranes during apoptosis. Cell Death Differ. 13:1277–1280. 2006. View Article : Google Scholar : PubMed/NCBI
25	Desagher S and Martinou JC: Mitochondria as the central control point of apoptosis. Trends Cell Biol. 10:369–377. 2000. View Article : Google Scholar : PubMed/NCBI
26	Takuma K, Baba A and Matsuda T: Astrocyte apoptosis: Implications for neuroprotection. Prog Neurobiol. 72:111–127. 2004. View Article : Google Scholar : PubMed/NCBI
27	Morimoto M, Nishida T, Honda R and Yasuda H: Modification of cullin-1 by ubiquitin-like protein Nedd8 enhances the activity of SCF^skp2 toward p27^kip1. Biochem Biophys Res Commun. 270:1093–1096. 2000. View Article : Google Scholar : PubMed/NCBI
28	Ray A, James MK, Larochelle S, Fisher RP and Blain SW: p27^Kip1 inhibits cyclin D-cyclin-dependent kinase 4 by two independent modes. Mol Cell Biol. 29:986–999. 2009. View Article : Google Scholar : PubMed/NCBI
29	Stahl M, Dijkers PF, Kops GJ, Lens SM, Coffer PJ, Burgering BM and Medema RH: The forkhead transcription factor FoxO regulates transcription of p27^Kip1 and Bim in response to IL-2. J Immunol. 168:5024–5031. 2002. View Article : Google Scholar : PubMed/NCBI