Functional expression, characterization, and application of human S100B

  • Authors:
    • Lei Wu
    • Xuesi Zhou
    • Zhigang Xiao
    • Xiujie Gao
    • Ziquan Liu
    • Zhiqing Zhang
    • Kun Wang
    • Yulin Zhu
    • Hanlin Ren
    • Tianhui Wang
  • View Affiliations

  • Published online on: August 24, 2017     https://doi.org/10.3892/or.2017.5922
  • Pages: 2309-2316
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Abstract

The EF-hand calcium-binding protein S100B presents a wide range of biological activities and functions. This binding protein is involved in various human diseases, including cancer, brain trauma and ischemia, neuro­degenerative disease (Alzheimer's disease), and psychiatric disorders. In this study, we prepared human S100B protein and its monoclonal antibodies. Human S100B protein was expressed in Escherichia coli, successfully purified by diethylaminoethyl cellulose anion-exchange chromatography, and then identified by western blot analysis. Monoclonal antibodies (mAbs) were produced by the standard hybridoma method and validated by enzyme-linked immunosorbent assay and western blot analysis. The prepared human S100B protein and its mAbs demonstrated potential biological activities. The KD of one mAb is approximately 4.72x10-8 mol/l, and its cross reactivity is low with human S100A4, mouse S100A4, and human S100A1. Recombinant Soluble S100B can promote the migration and invasion of HeLa cells. The expression of S100B protein in tumor tissues can be detected effectively by using the prepared monoclonal antibodies. Increasing concentration of the anti-human S100B mAbs showed a reduced expression of the S100B protein. Subsequently, the expression of p53 increased significantly (P<0.05) in A375 cells. A significant increase in apoptosis in A375 cells was observed with increasing S100B mAb concentration. Results showed that our prepared S100B mAbs were suitable for detecting S100B expression in human tissues, furnishing promising tools for further functional investigation and clinical applications.
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October-2017
Volume 38 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Wu L, Zhou X, Xiao Z, Gao X, Liu Z, Zhang Z, Wang K, Zhu Y, Ren H, Wang T, Wang T, et al: Functional expression, characterization, and application of human S100B . Oncol Rep 38: 2309-2316, 2017.
APA
Wu, L., Zhou, X., Xiao, Z., Gao, X., Liu, Z., Zhang, Z. ... Wang, T. (2017). Functional expression, characterization, and application of human S100B . Oncology Reports, 38, 2309-2316. https://doi.org/10.3892/or.2017.5922
MLA
Wu, L., Zhou, X., Xiao, Z., Gao, X., Liu, Z., Zhang, Z., Wang, K., Zhu, Y., Ren, H., Wang, T."Functional expression, characterization, and application of human S100B ". Oncology Reports 38.4 (2017): 2309-2316.
Chicago
Wu, L., Zhou, X., Xiao, Z., Gao, X., Liu, Z., Zhang, Z., Wang, K., Zhu, Y., Ren, H., Wang, T."Functional expression, characterization, and application of human S100B ". Oncology Reports 38, no. 4 (2017): 2309-2316. https://doi.org/10.3892/or.2017.5922