Biochanin-A induces apoptosis and suppresses migration in FaDu human pharynx squamous carcinoma cells

  • Authors:
    • In-A Cho
    • Sang-Joun You
    • Kyeong-Rok Kang
    • Su-Gwan Kim
    • Ji-Su Oh
    • Jae-Seek You
    • Gyeong-Je Lee
    • Yo-Seob Seo
    • Do Kyung Kim
    • Chun Sung Kim
    • Sook-Young Lee
    • Jae-Sung Kim
  • View Affiliations

  • Published online on: September 13, 2017     https://doi.org/10.3892/or.2017.5953
0

Abstract

The aim of the present study was to investigate biochanin-A-induced anticancer effects and their cellular signaling pathway in FaDu pharyngeal squamous carcinoma cells. Biochanin-A induced cell death through increased cytotoxicity of FaDu cells in a dose- and time-dependent manner. The number of cells with nucleus condensation and the apoptotic population were increased in the FaDu cells stimulated with biochanin-A for 24 h. Furthermore, extrinsic apoptotic factors such as FasL and their downstream target caspase-8 were increased and activated in the FaDu cells treated with biochanin-A in a dose-dependent manner. Moreover, biochanin-A decreased the expression of intrinsic anti-apoptotic factors such as Bcl-2 and Bcl-xL, and increased the level and activation of intrinsic apoptotic factors such as Bad and caspase-9. Finally, biochanin-A induced the activation of caspase-3 and Poly(ADP ribose) polymerase (PARP) in FaDu cells. Our results suggest that biochanin-A-induced apoptosis was mediated by death receptor mediated-extrinsic and mitochondria-dependent intrinsic apoptotic signaling pathways. Biochanin-A also inhibited wound healing migration and proliferation of FaDu cells via the downregulation and inactivation of matrix metalloproteinase-2 and -9 that are mediated by the suppression of p38, mitogen activated protein kinase (MAPK), NF-κB and Akt cellular signaling pathways. Therefore, these data suggest that the biochanin-A may act as a potential chemotherapeutic compound to treat head and neck cancer.

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Print ISSN: 1021-335X
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APA
Cho, I., You, S., Kang, K., Kim, S., Oh, J., You, J. ... Kim, J. (1899). Biochanin-A induces apoptosis and suppresses migration in FaDu human pharynx squamous carcinoma cells. Oncology Reports, 0, 0-0. https://doi.org/10.3892/or.2017.5953
MLA
Cho, I., You, S., Kang, K., Kim, S., Oh, J., You, J., Lee, G., Seo, Y., Kim, D. K., Kim, C. S., Lee, S., Kim, J."Biochanin-A induces apoptosis and suppresses migration in FaDu human pharynx squamous carcinoma cells". Oncology Reports 0.0 (1899): 0-0.
Chicago
Cho, I., You, S., Kang, K., Kim, S., Oh, J., You, J., Lee, G., Seo, Y., Kim, D. K., Kim, C. S., Lee, S., Kim, J."Biochanin-A induces apoptosis and suppresses migration in FaDu human pharynx squamous carcinoma cells". Oncology Reports 0, no. 0 (1899): 0-0. https://doi.org/10.3892/or.2017.5953