Biochanin-A induces apoptosis and suppresses migration in FaDu human pharynx squamous carcinoma cells

Cho,In-A; You,Sang-Joun; Kang,Kyeong-Rok; Kim,Su-Gwan; Oh,Ji-Su; You,Jae-Seek; Lee,Gyeong-Je; Seo,Yo-Seob; Kim,Do Kyung; Kim,Chun Sung; Lee,Sook-Young; Kim,Jae-Sung

doi:10.3892/or.2017.5953

Biochanin-A induces apoptosis and suppresses migration in FaDu human pharynx squamous carcinoma cells

Authors:
- In-A Cho
- Sang-Joun You
- Kyeong-Rok Kang
- Su-Gwan Kim
- Ji-Su Oh
- Jae-Seek You
- Gyeong-Je Lee
- Yo-Seob Seo
- Do Kyung Kim
- Chun Sung Kim
- Sook-Young Lee
- Jae-Sung Kim
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Affiliations: Department of Oral and Maxillofacial Surgery, School of Dentistry, Chosun University, Gwangju 61452, Republic of Korea, Department of Periodontology, School of Dentistry, Chosun University, Gwangju 61452, Republic of Korea, Department of Prosthodontics, School of Dentistry, Chosun University, Gwangju 61452, Republic of Korea, Department of Oral and Maxillofacial Radiology, School of Dentistry, Chosun University, Gwangju 61452, Republic of Korea, Oral Biology Research Institute, School of Dentistry, Chosun University, Gwangju 61452, Republic of Korea, Regional Innovation Center for Dental Science and Engineering, School of Dentistry, Chosun University, Gwangju 61452, Republic of Korea
Published online on: September 13, 2017 https://doi.org/10.3892/or.2017.5953
Pages: 2985-2992

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Abstract

The aim of the present study was to investigate biochanin-A-induced anticancer effects and their cellular signaling pathway in FaDu pharyngeal squamous carcinoma cells. Biochanin-A induced cell death through increased cytotoxicity of FaDu cells in a dose- and time-dependent manner. The number of cells with nucleus condensation and the apoptotic population were increased in the FaDu cells stimulated with biochanin-A for 24 h. Furthermore, extrinsic apoptotic factors such as FasL and their downstream target caspase-8 were increased and activated in the FaDu cells treated with biochanin-A in a dose-dependent manner. Moreover, biochanin-A decreased the expression of intrinsic anti-apoptotic factors such as Bcl-2 and Bcl-xL, and increased the level and activation of intrinsic apoptotic factors such as Bad and caspase-9. Finally, biochanin-A induced the activation of caspase-3 and Poly(ADP ribose) polymerase (PARP) in FaDu cells. Our results suggest that biochanin-A-induced apoptosis was mediated by death receptor mediated-extrinsic and mitochondria-dependent intrinsic apoptotic signaling pathways. Biochanin-A also inhibited wound healing migration and proliferation of FaDu cells via the downregulation and inactivation of matrix metalloproteinase-2 and -9 that are mediated by the suppression of p38, mitogen activated protein kinase (MAPK), NF-κB and Akt cellular signaling pathways. Therefore, these data suggest that the biochanin-A may act as a potential chemotherapeutic compound to treat head and neck cancer.

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