YWHAZ promotes ovarian cancer metastasis by modulating glycolysis

  • Authors:
    • Jie Shi
    • Jun Ye
    • He Fei
    • Shu‑Hen Jiang
    • Zhi‑Yong Wu
    • Ya‑Ping Chen
    • Li‑Wen Zhang
    • Xiao‑Mei Yang
  • View Affiliations

  • Published online on: December 7, 2018     https://doi.org/10.3892/or.2018.6920
  • Pages: 1101-1112
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Abstract

Ovarian cancer is one of the three most deadly gynecological cancers, with the highest mortality rate. As the main cause of death, metastasis is considered to be a crucial factor that reduces the survival time of ovarian carcinoma patients. YWHAZ (also known as 14‑3‑3ζ) influences diverse vital cellular processes such as metabolism, signal transduction, apoptosis and cell cycle regulation. In the present study, we determined that YWHAZ is upregulated in ovarian cancers in contrast to normal tissues by immunohistochemical staining. High YWHAZ expression was found to be associated with TNM stage and metastasis‑free prognosis of ovarian cancer patients. Silencing of YWHAZ inhibited the proliferation and facilitated serum starvation‑induced apoptosis of ovarian cancer cells. Cell migration was also suppressed by YWHAZ silencing. Furthermore, using an in vivo metastatic model, we found that YWHAZ silence also inhibited ovarian cancer metastasis in vivo. Notably, glycolysis was clearly inhibited in YWHAZ‑silenced ovarian cancer cells as determined by lactate production assay and Seahorse XF analysis. YWHAZ also regulated the PI3K/Akt1/vimentin signaling pathway in ovarian cancer cells as detected by western blot analysis. Taken together, our results demonstrated that YWHAZ plays an important role in the progression of ovarian cancer and can be used as a potential target for the diagnosis and treatment of epithelial ovarian cancer.
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February-2019
Volume 41 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Shi J, Ye J, Fei H, Jiang SH, Wu ZY, Chen YP, Zhang LW and Yang XM: YWHAZ promotes ovarian cancer metastasis by modulating glycolysis. Oncol Rep 41: 1101-1112, 2019.
APA
Shi, J., Ye, J., Fei, H., Jiang, S., Wu, Z., Chen, Y. ... Yang, X. (2019). YWHAZ promotes ovarian cancer metastasis by modulating glycolysis. Oncology Reports, 41, 1101-1112. https://doi.org/10.3892/or.2018.6920
MLA
Shi, J., Ye, J., Fei, H., Jiang, S., Wu, Z., Chen, Y., Zhang, L., Yang, X."YWHAZ promotes ovarian cancer metastasis by modulating glycolysis". Oncology Reports 41.2 (2019): 1101-1112.
Chicago
Shi, J., Ye, J., Fei, H., Jiang, S., Wu, Z., Chen, Y., Zhang, L., Yang, X."YWHAZ promotes ovarian cancer metastasis by modulating glycolysis". Oncology Reports 41, no. 2 (2019): 1101-1112. https://doi.org/10.3892/or.2018.6920