Open Access

PLCε promotes urinary bladder cancer cells proliferation through STAT3/LDHA pathway‑mediated glycolysis

  • Authors:
    • Honglin Cheng
    • Yanni Hao
    • Yingying Gao
    • Yunfeng He
    • Chunli Luo
    • Wei Sun
    • Mengjuan Yuan
    • Xiaohou Wu
  • View Affiliations

  • Published online on: March 13, 2019     https://doi.org/10.3892/or.2019.7056
  • Pages: 2844-2854
  • Copyright: © Cheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Phospholipase Cε (PLCε) and anaerobic glycolysis were determined to be involved in the development of human urinary bladder cancer (UBC), but the mechanisms remain unclear. In the present study, 64 bladder cancer specimens and 42 adjacent tissue specimens were obtained from 64 patients, and immunochemistry indicated that PLCε and lactate dehydrogenase (LDHA) are overexpressed in UBC. PLCε and LDHA were demonstrated to be positively correlated at transcription levels, indicating that one of these two genes may be regulated by another. To elucidate the mechanisms, PLCε was knocked down in T24 cells by short hairpin RNA, and then signal transducer and activator of transcription 3 (STAT3) phosphorylation and LDHA were determined to be downregulated, which indicated that PLCε may serve roles upstream of LDHA through STAT3 to regulate glycolysis in UBC. Furthermore, chromatin immunoprecipitation and luciferase reporter assays were performed to confirm that STAT3 could bind to the promoter of the LDHA gene to enhance its expression. A xenograft tumor mouse model also demonstrated similar results as the in vitro experiments, further confirming the role of PLCε in regulating bladder cell growth in vivo. Collectively, the present study demonstrated that PLCε may regulate glycolysis through the STAT3/LDHA pathway to take part in the development of human UBC.
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May-2019
Volume 41 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Cheng H, Hao Y, Gao Y, He Y, Luo C, Sun W, Yuan M and Wu X: PLCε promotes urinary bladder cancer cells proliferation through STAT3/LDHA pathway‑mediated glycolysis. Oncol Rep 41: 2844-2854, 2019.
APA
Cheng, H., Hao, Y., Gao, Y., He, Y., Luo, C., Sun, W. ... Wu, X. (2019). PLCε promotes urinary bladder cancer cells proliferation through STAT3/LDHA pathway‑mediated glycolysis. Oncology Reports, 41, 2844-2854. https://doi.org/10.3892/or.2019.7056
MLA
Cheng, H., Hao, Y., Gao, Y., He, Y., Luo, C., Sun, W., Yuan, M., Wu, X."PLCε promotes urinary bladder cancer cells proliferation through STAT3/LDHA pathway‑mediated glycolysis". Oncology Reports 41.5 (2019): 2844-2854.
Chicago
Cheng, H., Hao, Y., Gao, Y., He, Y., Luo, C., Sun, W., Yuan, M., Wu, X."PLCε promotes urinary bladder cancer cells proliferation through STAT3/LDHA pathway‑mediated glycolysis". Oncology Reports 41, no. 5 (2019): 2844-2854. https://doi.org/10.3892/or.2019.7056