Open Access

Weighted gene coexpression analysis indicates that PLAGL2 and POFUT1 are related to the differential features of proximal and distal colorectal cancer

  • Authors:
    • Yiming Lv
    • Binbin Xie
    • Bingjun Bai
    • Lina Shan
    • Wenqian Zheng
    • Xuefeng Huang
    • Hongbo Zhu
  • View Affiliations

  • Published online on: October 14, 2019     https://doi.org/10.3892/or.2019.7368
  • Pages: 2473-2485
  • Copyright: © Lv et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

In the current era of precision medicine, there is a general consensus that the anatomical site is an important factor in the management of colorectal cancer (CRC). To investigate the underlying molecular mechanisms between proximal and distal CRC and to identify the responsible genes, we analyzed the gene expression patterns of colorectal tumors from two microarray datasets, GSE39582 and GSE14333, on the NCBI Gene Expression Omnibus and the RNA‑seq data from TCGA. Weighted coexpression network analysis (WGCNA) was applied to construct a gene coexpression network. The red module in GSE39582 and the dark‑gray module from the TCGA dataset were found to be highly correlated with the anatomical site of CRC. A total of 12 hub genes were found in two datasets, 2 of which PLAG1 like zinc finger 2 (PLAGL2) and protein O‑fucosyltransferase 1 (POFUT1) were common and upregulated in tumor samples in CRC. The module with the highest correlation provided references that will help to characterize the difference between left‑sided and right‑sided CRC. The survival analysis of PLAGL2 and POFUT1 expression revealed differences between proximal and distal CRC. Gene set enrichment analysis based on those two genes provided similar results: GPI anchor biosynthesis and peroxisome and selenoamino acid metabolism. PLAGL2 and POFUT1, which have the highest correlation with tumor location, may serve as biomarkers and therapeutic targets for the precise diagnosis and treatment of CRC in the future.
View Figures
View References

Related Articles

Journal Cover

December-2019
Volume 42 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Lv Y, Xie B, Bai B, Shan L, Zheng W, Huang X and Zhu H: Weighted gene coexpression analysis indicates that PLAGL2 and POFUT1 are related to the differential features of proximal and distal colorectal cancer. Oncol Rep 42: 2473-2485, 2019.
APA
Lv, Y., Xie, B., Bai, B., Shan, L., Zheng, W., Huang, X., & Zhu, H. (2019). Weighted gene coexpression analysis indicates that PLAGL2 and POFUT1 are related to the differential features of proximal and distal colorectal cancer. Oncology Reports, 42, 2473-2485. https://doi.org/10.3892/or.2019.7368
MLA
Lv, Y., Xie, B., Bai, B., Shan, L., Zheng, W., Huang, X., Zhu, H."Weighted gene coexpression analysis indicates that PLAGL2 and POFUT1 are related to the differential features of proximal and distal colorectal cancer". Oncology Reports 42.6 (2019): 2473-2485.
Chicago
Lv, Y., Xie, B., Bai, B., Shan, L., Zheng, W., Huang, X., Zhu, H."Weighted gene coexpression analysis indicates that PLAGL2 and POFUT1 are related to the differential features of proximal and distal colorectal cancer". Oncology Reports 42, no. 6 (2019): 2473-2485. https://doi.org/10.3892/or.2019.7368