Open Access

Long non‑coding RNA PCAT6 promotes the development of osteosarcoma by increasing MDM2 expression

  • Authors:
    • Xiliang Guan
    • Yufen Xu
    • Jufen Zheng
  • View Affiliations

  • Published online on: October 16, 2020     https://doi.org/10.3892/or.2020.7813
  • Pages: 2465-2474
  • Copyright: © Guan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Osteosarcoma is a severe malignant tumor. Several studies indicated that lncRNA prostate cancer‑associated transcript 6 (PCAT6) promoted the development of multiple types of cancers. Studies have also revealed that MDM2 could aggravate tumor symptoms inhibiting P53 expression. However, whether lncRNA PCAT6 could affect the proliferation and metastasis of osteosarcoma cells by regulating P53 expression is unclear. The present study established lncRNA PCAT6‑overexpressing osteosarcoma cells. Cell Counting Kit‑8, wound healing and Transwell assays were performed to detect the change in proliferation, migration and invasion of these cells, respectively. Subsequently, E3 ubiquitin‑protein ligase Mdm2 (MDM2), P53 and P21 expression were determined using western blotting. Finally, MDM2 expression was inhibited and the proliferation, migration and invasion of these cells was determined again. The present study found that the proliferation, migration and invasion of osteosarcoma cells increased following overexpression of lncRNA PCAT6. MDM2 expression was upregulated while the levels of P53 and P21 decreased following overexpression of lncRNA PCAT6. However, the proliferation, migration and invasion of osteosarcoma cells were inhibited following MDM2 knockdown. Additionally, P53 and P21 was rescued following MDM2 knockdown. To conclude, lncRNA PCAT6 promoted the proliferation, migration and invasion of osteosarcoma cells by promoting the expression of MDM2 and suppressing the expression of P53 and P21.
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December-2020
Volume 44 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Guan X, Xu Y and Zheng J: Long non‑coding RNA PCAT6 promotes the development of osteosarcoma by increasing MDM2 expression. Oncol Rep 44: 2465-2474, 2020.
APA
Guan, X., Xu, Y., & Zheng, J. (2020). Long non‑coding RNA PCAT6 promotes the development of osteosarcoma by increasing MDM2 expression. Oncology Reports, 44, 2465-2474. https://doi.org/10.3892/or.2020.7813
MLA
Guan, X., Xu, Y., Zheng, J."Long non‑coding RNA PCAT6 promotes the development of osteosarcoma by increasing MDM2 expression". Oncology Reports 44.6 (2020): 2465-2474.
Chicago
Guan, X., Xu, Y., Zheng, J."Long non‑coding RNA PCAT6 promotes the development of osteosarcoma by increasing MDM2 expression". Oncology Reports 44, no. 6 (2020): 2465-2474. https://doi.org/10.3892/or.2020.7813