Lipid droplet storage promotes murine pancreatic tumor growth

  • Authors:
    • Jeremy J. Grachan
    • Martin Kery
    • Amato J. Giaccia
    • Nicholas C. Denko
    • Ioanna Papandreou
  • View Affiliations

  • Published online on: February 9, 2021     https://doi.org/10.3892/or.2021.7972
  • Article Number: 21
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Hypoxia Inducible Lipid Droplet Associated (HILPDA) is frequently overexpressed in tumors and promotes neutral lipid storage. The impact of Hilpda on pancreatic ductal adenocarcinoma (PDAC) tumor growth is not known. In order to evaluate Hilpda‑dependent lipid storage mechanisms, expression of Hilpda in murine pancreatic cells (KPC) was genetically manipulated. Lipid droplet (LD) abundance and triglyceride content in vitro were measured, and model tumor growth in nu/nu mice was determined. The results showed that excess lipid supply increased triglyceride storage and LD formation in KPC cells in a HILPDA‑dependent manner. Contrary to published results, inhibition of Adipose Triglyceride Lipase (ATGL) did not ameliorate the triglyceride abundance differences between Hilpda WT and KO cells. Hilpda ablation significantly decreased the growth rate of model tumors in immunocompromised mice. In conclusion, Hilpda is a positive regulator of triglyceride storage and lipid droplet formation in murine pancreatic cancer cells in vitro and lipid accumulation and tumor growth in vivo. Our data suggest that deregulated ATGL is not responsible for the absence of LDs in KO cells in this context.
View Figures
View References

Related Articles

Journal Cover

April-2021
Volume 45 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Grachan JJ, Kery M, Giaccia AJ, Denko NC and Papandreou I: Lipid droplet storage promotes murine pancreatic tumor growth. Oncol Rep 45: 21, 2021.
APA
Grachan, J.J., Kery, M., Giaccia, A.J., Denko, N.C., & Papandreou, I. (2021). Lipid droplet storage promotes murine pancreatic tumor growth. Oncology Reports, 45, 21. https://doi.org/10.3892/or.2021.7972
MLA
Grachan, J. J., Kery, M., Giaccia, A. J., Denko, N. C., Papandreou, I."Lipid droplet storage promotes murine pancreatic tumor growth". Oncology Reports 45.4 (2021): 21.
Chicago
Grachan, J. J., Kery, M., Giaccia, A. J., Denko, N. C., Papandreou, I."Lipid droplet storage promotes murine pancreatic tumor growth". Oncology Reports 45, no. 4 (2021): 21. https://doi.org/10.3892/or.2021.7972