Open Access

Evaluation of the effect of oral administration of collagen peptides on an experimental rat osteoarthritis model

  • Authors:
    • Satoko Isaka
    • Akimasa Someya
    • Shinji Nakamura
    • Kiyohito Naito
    • Masahiko Nozawa
    • Naoki Inoue
    • Fumihito Sugihara
    • Isao Nagaoka
    • Kazuo Kaneko
  • View Affiliations

  • Published online on: April 5, 2017     https://doi.org/10.3892/etm.2017.4310
  • Pages: 2699-2706
  • Copyright: © Isaka et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Collagen is an extracellular matrix protein present in the skin, tendon, cartilage and bone. Collagen peptides (CP) are produced by the hydrolysis of gelatin (heat‑denatured collagen) by proteases and are utilized as a component of nutraceuticals. The current study investigated the effect of CP on the articular cartilage of OA by evaluating the serum levels of biomarkers (CTX‑II for type II collagen degradation and CPII for type II collagen synthesis), histopathological changes (Mankin score, based on the toluidine blue staining of proteoglycans), and immunohistochemical staining of matrix metalloproteinase (MMP)‑13 and type II collagen, using a rat experimental osteoarthritis (OA) model. Anterior cruciate ligament transection (ACLT) was performed on the right knee joint to surgically induce OA. Animals were divided into four groups: Control group (Control), sham‑operated group (Sham), ACLT group without collagen peptide (ACLT group) and ACLT group with oral administration of CP (CP group). ACLT induced histological damages and significantly increased the Mankin score (P<0.05). However, CP administration markedly suppressed the Mankin score, although this difference was not significant. In addition, serum CTX‑II levels were significantly decreased in CP group compared with those in the ACLT group (P<0.05). By contrast, serum CPII levels did not differ significantly among the four groups. Moreover, immunohistochemical staining of type II collagen and MMP‑13 (an important type II collagen‑degrading enzyme) indicated that the amount of type II collagen increased, whereas the number of MMP‑13 positive chondrocytes decreased in the CP group compared with ACLT group. These observations suggest that CP has the potential to exert chondroprotective action on OA by inhibiting MMP‑13 expression and type II collagen degeneration.

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June-2017
Volume 13 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Isaka S, Someya A, Nakamura S, Naito K, Nozawa M, Inoue N, Sugihara F, Nagaoka I and Kaneko K: Evaluation of the effect of oral administration of collagen peptides on an experimental rat osteoarthritis model. Exp Ther Med 13: 2699-2706, 2017
APA
Isaka, S., Someya, A., Nakamura, S., Naito, K., Nozawa, M., Inoue, N. ... Kaneko, K. (2017). Evaluation of the effect of oral administration of collagen peptides on an experimental rat osteoarthritis model. Experimental and Therapeutic Medicine, 13, 2699-2706. https://doi.org/10.3892/etm.2017.4310
MLA
Isaka, S., Someya, A., Nakamura, S., Naito, K., Nozawa, M., Inoue, N., Sugihara, F., Nagaoka, I., Kaneko, K."Evaluation of the effect of oral administration of collagen peptides on an experimental rat osteoarthritis model". Experimental and Therapeutic Medicine 13.6 (2017): 2699-2706.
Chicago
Isaka, S., Someya, A., Nakamura, S., Naito, K., Nozawa, M., Inoue, N., Sugihara, F., Nagaoka, I., Kaneko, K."Evaluation of the effect of oral administration of collagen peptides on an experimental rat osteoarthritis model". Experimental and Therapeutic Medicine 13, no. 6 (2017): 2699-2706. https://doi.org/10.3892/etm.2017.4310