Oxymatrine attenuates oxidized low‑density lipoprotein‑induced HUVEC injury by inhibiting NLRP3 inflammasome‑mediated pyroptosis via the activation of the SIRT1/Nrf2 signaling pathway
- Authors:
- Published online on: August 4, 2021 https://doi.org/10.3892/ijmm.2021.5020
- Article Number: 187
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Copyright: © Jin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Abstract
Introduction
Atherosclerosis, a chronic inflammatory disease, is an important pathological process involved in peripheral artery disease, coronary artery disease and stroke, and has been attracting research interest during recent years (1,2). A substantial number of studies have indicated that the NLR family pyrin domain containing 3 (NLRP3) inflammasome activation is implicated in the vascular inflammatory response driving the development and progression of atherosclerosis (3,4). Upon activation, NLRP3 interacts with adapter apoptosis-associated speck-like protein containing a C-terminal caspase recruitment domain (ASC) to form the NLRP3 inflammasome, resulting in caspase-1 activation and the release of proinflammatory cytokines [i.e., interleukin (IL)-1β and IL-18], a process referred to as NLRP3 inflammasome activation-mediated pyroptosis (5-7), which has been reported as an important event in endothelial injury (8,9) and the pathophysiological process of atherosclerosis (10,11). Therefore, mitigation of NLRP3 inflammasome activation-mediated pyroptosis may be a promising therapeutic strategy for reversing endothelial injury in atherosclerosis.
Oxymatrine, a quinolizidine alkaloid isolated from the traditional Chinese herb Sophora flavescens Aiton, possesses multiple pharmacological properties, such as anti-inflammatory, antioxidant, antifibrotic and cardiovascular protective effects (12-14). To date, oxymatrine has been extensively discussed for its protective effects against heart injury induced by doxorubicin (14), chronic heart failure (15), aldosterone (16) and hypertension (17). Recent studies have claimed that oxymatrine inhibits homocysteine-induced human umbilical vein endothelial cell (HUVEC) apoptosis/death, attenuating endothelial cell dysfunction (a hallmark of atherosclerosis) (18,19), thereby providing new insight into the role of oxymatrine in the prevention of atherosclerosis; however, the precise mechanisms have yet to be fully elucidated. Hence, the present study was undertaken to investigate the protective effect of oxymatrine against oxidized low-density lipoprotein (ox-LDL)-induced HUVEC injury, a typical in vitro model of atherosclerosis (20), and further demonstrated the role of NLRP3 inflammasome activation-mediated pyroptosis in this process.
Sirtuin (SIRT) 1, a member of the sirtuin family of NAD+-dependent deacetylases, has long been considered as an anti-atherosclerosis factor (21-23). SIRT1 can activate the nuclear factor-erythroid 2-related factor 2 (Nrf2) signaling pathway, and the activation of the Nrf2 pathway has been shown to attenuate endothelial cell injury, inhibit inflammation and exert anti-atherosclerotic effects (24,25). Once activated, the Nrf2 signaling pathway can also inhibit NLRP3-mediated pyroptosis (26,27). However, the exact effect of the SIRT1/Nrf2 signaling pathway on NLRP3 inflammasome activation-mediated pyroptosis in atherosclerosis has yet to be reported. Notably, it has been confirmed that oxymatrine can activate the SIRT1 signaling pathway (28,29). Hence, the present study further explored the role of the SIRT1/Nrf2 signaling pathway in the protective effect of oxymatrine against ox-LDL injury and the inhibitory effect of oxymatrine on NLRP3 inflammasome activation-mediated pyroptosis in HUVECs.
The results of the present study may improve our understanding of the protective properties of oxymatrine agaisnt ox-LDL injury, indicating potential novel approaches to the treatment of atherosclerosis.
Materials and methods
Materials
ox-LDL, 2′,7′-dichlorofluorescein diacetate (DCFH-DA) and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich; Merck KGaA. Oxymatrine (purity, >98%) was obtained from Green Valley Pharmaceutical Co., Ltd. A stock suspension of oxymatrine (50 mg/ml) was prepared by dilution with PBS and stored at 4°C. Dulbecco’s modified Eagle’s medium (DMEM; cat. no. SH30022.01B) was purchased from HyClone; Cytiva. Fetal bovine serum (FBS; cat. no. 16140071), doxorubicin and MTT assay kit (cat. no. V13154) were purchased from Gibco; Thermo Fisher Scientific, Inc. Annexin V-FITC/PI Apoptosis Detection kit was obtained from Shanghai Yisheng Technology Co., Ltd. (cat. no. 40302ES50). TNF-α assay kit (cat. no. H052), IL-1β assay kit (cat. no. H002), IL-6 assay kit (cat. no. H007) and IL-10 assay kit (cat. no. H009) were obtained from Nanjing Jiangcheng Bioengineering Institute. Anti-Bax (product no. 2772), anti-Bcl-2 (product no. 15071), anti-NLRP3 (product no. 13158), anti-cleaved caspase-1 (product no. 89332), anti-caspase-1 (product no. 24232) anti-IL-1β (product no. 12703), anti-IL-18 (product no. 54943), anti-SIRT1 (product no. 8469), anti-Nrf2 (product no. 12721), anti-heme oxygenase (HO)-1 (product no. 43966), anti-cleaved caspase-3 (product no. 9661), anti-caspase-3 (product no. 9662), anti-GAPDH (product no. 5174) and anti-histone H3 (product no. 4499) antibodies were purchased from Cell Signaling Technology, Inc. Small interfering (si)RNA for NLRP3 (siNLRP3; cat. no. sc-45469) and control siRNA-B (siNC; cat. no. sc-44230), siRNA for SIRT1 (siSIRT1; cat. no. sc-40986) and control siRNA-A (siCon; cat. no. sc-37007) were purchased from Santa Cruz Biotechnology, Inc.
HUVEC culture and treatment
HUVECs were purchased from the American Type Culture Collection and cultured in DMEM supplemented with 10% (v/v) FBS and 1% penicillin/streptomycin at 37°C in a humidified 5% CO2 incubator. The culture medium was replaced every 1-2 days. HUVECs were treated with ox-LDL (100 µg/ml) for 24 h at 37°C to mimic atherosclerotic conditions in vitro (30). To detect the possible protective effects of oxymatrine against ox-LDL injury, HUVECs were pre-treated with oxymatrine (2, 4 or 8 µM) for 1 h prior to ox-LDL (100 µg/ml) challenge for 24 h at 37°C. To investigate the role of NLRP3 inflammasome activation-mediated pyroptosis in the protective effect of oxymatrine against ox-LDL-induced injury, HUVECs were transfected with siRNAs for NLRP3 (siNLRP3) followed by oxymatrine (4 µM) for 1 h prior to ox-LDL (100 µg/ml) challenge for 24 h. To demonstrate the function of the SIRT1/Nrf2 signaling pathway in the protective effect of oxymatrine against ox-LDL injury, HUVECs were transfected with siRNAs for SIRT1 (siSIRT1) followed by oxymatrine (4 µM) for 1 h prior to ox-LDL (100 µg/ml) challenge for 24 h. The dose of oxymatrine selected was according to our pilot study (unpublished data).
siRNA transfection
HUVECs were incubated in 6-well plates at 37°C overnight at a density of 1×105 cells/well, and then transfected with siRNAs for NLRP3 (siNLRP3, 50 nM) or siRNA for SIRT1 (siSIRT1, 50 nM), as well as a respective negative control (siNC, 50 nM) using Lipofectamine® 2000 reagent (Invitrogen; Thermo Fisher Scientific, Inc.) following the manufacturer’s protocol. The transfection sequences were as follows: siNLRP3 sense, 5′-GCU UCA GCC ACA UGA CUU UTT-3′ and antisense, 5′-AAA GUC AUG UGG CUG AAG CTT-3′; siNC sense, 5′-UUC UCC GAA CGU GUC ACG UTT-3′ and antisense, 5′-ACG UGA CAC GUU CGG AGA ATT-3′; siSIRT1 sense, 5′-UUG GGA UUC ACG CAU AGG AGC ACUG-3′ and antisense, 5′-CAG UGC UCC UAU GCG GAA UCC CAA-3′; and siRNA for control (siCon), sense 5′-UUC UCC GAA CGU GUC ACG UdT dT-3′ and antisense 5′-ACG UGA CAC GUU CGG AGA AdT dT-3′. In brief, siNLRP3 (50 nM), siSIRT1 (50 nM), siNC (50 nM) or siCon (50 nM) was mixed with Lipofectamine® 2000 transfection reagent, and the mixture was added to the cells and incubated for 6 h at 37°C. Then the medium was replaced with complete medium and the cells were cultured for 24 h, following which the cells were collected for subsequent experiments. The transfection efficiency of siNLRP3 and siSIRT1 in HUVECs was detected at 24 h after transfection by western blotting.
Cell viability assay
The viability of HUVECs was measured by Cell Counting Kit-8 (CCK-8) assay. In brief, HUVECs were grown in 96-well plates and treated as aforementioned. CCK-8 solution (10 µl; cat. no. C0038; Beyotime Biotechnology) was added to each well and co-incubated for 3 h at 37°C. The optical density (OD) at 450 nm was measured using a microplate reader (Thermo Fisher Scientific, Inc.).
Lactate dehydrogenase (LDH) assay
The cell death was further detected using an LDH Cytotoxicity Assay kit (cat. no. C0017; Beyotime Biotechnology) according to the manufacturer’s protocol. The LDH release was measured at 490 nm using a microplate reader (Bio-Rad Laboratories, Inc.).
Apoptosis assay
The apoptosis ratios of HUVECs were measured using Annexin V-FITC/PI Apoptosis Detection kit by flow cytometry according to manufacturer’s instructions. Briefly, after treatment, HUVECs were collected, washed twice with PBS, and incubated with Annexin V-FITC (10 µl) and PI (5 µl) for 20 min at room temperature in the dark. The mixture was detected by flow cytometric analysis using a flow cytometer (FACSCalibur™; BD Biosciences), and the data were analyzed using FlowJo 7.6 software (Tree Star, Inc.).
Determination of reactive oxygen species (ROS) production
The production of intracellular ROS was detected by the DCFH-DA fluorescence probe according to the manufacturer’s protocol. In brief, the treated HUVECs were harvested and washed twice with PBS, and then incubated with DCFH-DA (10 µM) for 20 min in the dark at 37°C. After washing again with PBS, images were captured under a fluorescence microscope (80i; Nikon Corporation). In addition, the mean fluorescence intensity of ROS was analyzed and averaged using flow cytometry (FACSCalibur™), and the data were analyzed by FlowJo 7.6 software (Tree Star, Inc.).
Determination of malondialdehyde (MDA) content, super-oxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities
After treatment, HUVECs were harvested, washed with ice-cold PBS, and incubated with the lysis buffer provided in the assay kits for 30 min on ice. Subsequently, cell lysates were centrifuged at 10,000 × g for 10 min at 4°C, the supernatant was collected and the protein content was measured using bicinchoninic acid (BCA) protein assay kit (Beyotime Biotechnology). The MDA content (cat. no. A003-1-2), SOD (cat. no. A001-3-2), CAT (cat. no. A007-1-1) and GSH-Px (cat. no. A005-1-2) activities were determined by commercial kits from Nanjing Jiancheng Bioengineering Institute according to the manufacturers’ protocol.
Measurement of mitochondrial membrane potential (MMP)
The MMP was monitored using the JC-1 dye kit according to the manufacturers’ protocol (cat. no. C2006; Beyotime Biotechnology). Briefly, HUVECs were harvested, washed twice with PBS, and then incubated with JC-1 for 20 min at 37°C in the dark. The data were analyzed and averaged using flow cytometry (FACSCalibur™), and the data were analyzed by FlowJo 7.6 software (Tree Star, Inc.).
Western blot analysis
Total protein from HUVECs was isolated using RIPA lysis buffer (Beyotime Biotechnology) containing 1% (v/v) protease inhibitor cocktail (Roche Diagnostics). The cytoplasmic and nuclear proteins of HUVECs were extracted with a cytoplasmic and nuclear extraction kit (cat. no. CW0199), according to the manufacturer’s instructions (CWBIO). The protein concentration was measured with a BCA protein assay kit. Equal amounts of protein (30 µg) were separated by 12% SDS-PAGE and then transferred onto PVDF membranes. After blocking with 5% (w/v) skimmed dry milk powder dissolved in Tris-buffered saline containing 0.1% (v/v) Tween-20 for 2 h at room temperature, the membranes were incubated with primary antibodies against Bax (1:2,000), Bcl-2 (1:2,000), NLPR3 (1:1,000), ASC (1:1,000), cleaved caspase-1 (1:1,000), caspase-1 (1:2,000) IL-1β (1:1,000), IL-18 (1:1,000), SIRT1 (1:2,000), Nrf2 (1:1,000), HO-1 (1:1,000), cleaved caspase-3 (1:2,000), caspase-3 (1:1,000), GAPDH (1:2,000) and histone H3 (1:2,000) overnight at 4°C. GAPDH was used as an internal control. Then, the membranes were incubated with HRP-conjugated secondary antibodies (1:5,000; product no. 7074; Cell Signaling Technology, Inc.) for 2 h at room temperature and the probed protein was visualized by Beyo ECL Plus (cat. no. P0018, Beyotime Biotechnology). The densitometric analysis was semi-quantified with ImageJ 1.43 software (National Institutes of Health).
Statistical analysis
Statistical analysis was performed by GraphPad Prism 5 (GraphPad Software, Inc.) using one-way analysis of variance (ANOVA) followed by Tukey’s post hoc test. All the data are presented as the mean ± SD. All experiments were repeated at least three times. P<0.05 was considered to indicate a statistically significant difference.
Results
Oxymatrine alleviates ox-LDL-induced HUVEC injury
To assess the protective effect of oxymatrine against ox-LDL-induced injury in HUVECs, cells were pre-treated with varying concentrations of oxymatrine in the presence or absence of ox-LDL (100 µg/ml). The results revealed that oxymatrine (2, 4 or 8 µM) significantly reversed the ox-LDL-induced decrease in cell viability (Fig. 1A). Treatment with oxymatrine alone (2, 4 or 8 µM) did not affect cell viability compared with the control group. The results revealed that oxymatrine (4 µM) obviously increased the viability of HUVECs treated with ox-LDL (P<0.01), which was selected as an optimal dose for interference with HUVECs treated with ox-LDL in a follow-up experiment. Subsequently, the result confirmed that oxymatrine (4 µM) significantly attenuated the ox-LDL-induced increase in LDH release in HUVECs (Fig. 1B). Flow cytometric assay results further demonstrated that oxymatrine pretreatment also suppressed the ox-LDL-induced upregulation of the apoptotic percentage in HUVECs (Fig. 1C). Bcl-2 and Bax are important members of the Bcl-2 family and they play a key role in the mitochondrial-mediated apoptotic pathway (31). The results of western blot analysis (Fig. 1D) further demonstrated that ox-LDL treatment resulted in an increase in the expression of the pro-apoptotic protein Bax (Fig. 1E) and a decrease in the expression of the anti-apoptotic protein Bcl-2 (Fig. 1F), while these effects were significantly blocked by oxymatrine. These data indicated that oxymatrine protected HUVECs against ox-LDL-induced injury.
Oxymatrine inhibits NLRP3 inflammasome-mediated pyroptosis in HUVECs subjected to ox-LDL treatment
Several studies have revealed the important role of NLRP3 inflammasome in atherosclerosis (4,32). The present study further investigated whether oxymatrine has the ability to reduce NLRP3 inflammasome activation during atherosclerosis. As shown in Fig. 2A, compared with the ox-LDL group, pretreatment with oxymatrine significantly decreased the expression of NLRP3 (Fig. 2B) and ASC (Fig. 2C) in HUVECs. The research confirmed that NLRP3 inflammasome activation leads to caspase-1 activation, thereby leading to maturation of effector pro-inflammatory cytokines, such as IL-1β and IL-18; thus, activated caspase-1 indicates the presence of pyroptosis (7). The results of western blot analysis (Fig. 2D) further demonstrated that ox-LDL resulted in the upregulation of the ratio of cleaved caspase-1/caspase-1 (Fig. 2E), IL-1β (Fig. 2F) and the expression of IL-18 (Fig. 2G) in HUVECs, while these effects were blocked by oxymatrine. These results indicated that oxymatrine attenuated NLRP3 inflammasome-mediated pyroptosis under conditions of ox-LDL stimulation.
Furthermore, to investigate the exact role of NLRP3 inflammasome-mediated pyroptosis in the protection of oxymatrine against ox-LDL injury, HUVECs were transfected with siNLRP3. The results demonstrated that siNLRP3 transfection successfully decreased the expression of NLRP3, ASC, cleaved caspase-1, IL-1β and IL-18 protein (Fig. 2H) in HUVECs. Moreover, siNLRP3 transfection also mitigated the ox-LDL-induced decrease in cell viability (Fig. 2I), the increase in LDH release (Fig. 2J) and apoptosis rate (Fig. 2K) of HUVECs, indicating that inhibition of NLRP3 inflammasome activation-mediated pyroptosis attenuated ox-LDL-induced HUVEC injury. Collectively, these findings indicated that oxymatrine protected HUVECs against ox-LDL-induced injury through inhibition of NLRP3 inflammasome activation-mediated pyroptosis.
Oxymatrine promotes SIRT1/Nrf2 signaling pathway activation in HUVECs subjected to ox-LDL treatment
To further investigate the mechanism underlying oxymatrine-induced endothelial protection against ox-LDL injury, the effects of oxymatrine on the SIRT1/Nrf2 signaling pathway in HUVECs were investigated. As revealed in Fig. 3, ox-LDL treatment resulted in the downregulation of SIRT1 expression in HUVECs, while oxymatrine pretreatment led to the upregulation of SIRT expression (Fig. 3A and B) in ox-LDL-treated HUVECs. In addition, oxymatrine blocked the ox-LDL-induced increase in cytoplasmic Nrf2 (C-Nrf2) expression (Fig. 3A and C) and decrease in nuclear Nrf2 (N-Nrf2) expression (Fig. 3E and F), indicating that oxymatrine may promote Nrf2 nuclear translocation. In addition, the ox-LDL-induced downregulation of the expression of the Nrf2-related antioxidant defense enzyme HO-1 was also reversed by oxymatrine (Fig. 3A and D). These results indicated that oxymatrine promoted the activation of the SIRT1/Nrf2 signaling pathway under conditions of ox-LDL stimulation in HUVECs.
Depletion of the SIRT1 signaling pathway blocks the protective effect of oxymatrine against ox-LDL-induced injury in HUVECs
To further explore the pathophysiological significance of oxymatrine-induced SIRT1/Nrf2 signaling pathway activation, SIRT1 knockdown was performed by transfection of siSIRT1 to further investigate the effect of oxymatrine against ox-LDL-induced injury. The results (Fig. 4A) demonstrated that siSIRT1 transfection markedly reduced the expression of SIRT1 compared with siCon transfection in HUVECs treated with oxymatrine and ox-LDL (Fig. 4B). siSIRT1 transfection also decreased the expression of HO-1 (Fig. 4C) and N-Nrf2 (Fig. 4D) in comparison with siCon transfection, indicating that siSIRT1 transfection successfully led to the deficiency of the SIRT1/Nrf2 signaling pathway. On this basis, the results further revealed that siSIRT transfection inhibited the oxymatrine-induced increase in cell viability (Fig. 4E) and the decrease in LDH release (Fig. 4F) in ox-LDL-treated HUVECs compared with siCon transfection. Furthermore, the inhibitory effect of oxymatrine on apoptosis rate was also alleviated by siSIRT1 transfection in comparison with siCon transfection (Fig. 4G). Caspase-3 is the main executioner of apoptosis, and activated caspase-3 can induce the cells to undergo apoptosis (33). Oxymatrine reduced the ox-LDL-induced increase in the ratio of cleaved caspase-3/caspase-3, which was also reversed by siSIRT1 (Fig. 4H and I). Collectively, these data indicated that the SIRT1/Nrf2 signaling pathway may contribute to the protection of oxymatrine against ox-LDL injury in HUVECs.
Inhibition of the SIRT1/Nrf2 signaling pathway eliminates the oxymatrine-induced protective effect against oxidative stress in ox-LDL-stimulated HUVECs
The present study further investigated the role of the SIRT1/Nrf2 signaling pathway in the antioxidant activity of oxymatrine in atherosclerosis. As revealed in Fig. 5, oxymatrine pretreatment significantly reversed the ox-LDL-induced increase in endogenous ROS generation (Fig. 5A and B) and MDA content (Fig. 5C), a major marker of lipid peroxidation. However, these effects of oxymatrine were both blocked by siSIRT1 transfection. In addition, oxymatrine resulted in an increase in MMP in HUVECs subjected to ox-LDL, which was also reversed by siSIRT1 transfection (Fig. 5D). Excessive ROS production and/or failure of antioxidant defense systems lead to oxidative stress and cardiomyocyte injury (34). Hence, the effects of oxymatrine on antioxidant enzymes inducing SOD, CAT and GSH-Px activity and the role of the SIRT1/Nrf2 signaling pathway in this process were explored. The results demonstrated that oxymatrine pretreatment obviously attenuated the ox-LDL-induced decrease in SOD (Fig. 5E), GSH-Px (Fig. 5F) and CAT (Fig. 5G) activities in HUVECs. However, oxymatrine-induced increases in SOD (Fig. 5E), GSH-Px (Fig. 5F) and CAT (Fig. 5G) activities were blocked by siSIRT1 transfection. Collectively, these results indicated that the SIRT1/Nrf2 signaling pathway mediated the protective effects of oxymatrine against ox-LDL-induced oxidative damage in HUVECs.
Inhibition of the SIRT1/Nrf2 signaling pathway alleviates oxymatrine-reduced NLRP3 inflammasome-mediated pyroptosis in ox-LDL-stimulated HUVECs
Research has confirmed that SIRT1 signaling inhibits NLRP3 inflammasome activation in vascular endothelial cells (35). The present study was undertaken to further explore whether oxymatrine attenuates NLRP3 inflammasome activation-mediated pyroptosis in ox-LDL-stimulated HUVECs via promoting SIRT1/Nrf2 signaling. The findings (Fig. 6A) revealed that inactivation of the SIRT1/Nrf2 signaling pathway induced by siSIRT1 transfection weakened the oxymatrine-induced decrease in the expression of NLRP3 (Fig. 6B) and ASC (Fig. 6C) in ox-LDL-treated HUVECs. Transfection with siSIRT1 also inhibited the oxymatrine-induced decrease in the ratio of cleaved caspase-1/caspase-1 (Fig. 6D and E) and the expression of IL-1β (Fig. 6D and F) and IL-18 (Fig. 6D and G) in ox-LDL-treated HUVECs. These results indicated that the SIRT1/Nrf2 signaling pathway mediated the inhibitory effect of oxymatrine on NLRP3 inflammasome-mediated pyroptosis in ox-LDL-treated HUVECs.
Discussion
The present study investigated the protective effects of oxymatrine against ox-LDL-induced injury in HUVECs, and further demonstrated the underlying mechanisms, focusing on NLRP3 inflammasome-mediated pyroptosis and SIRT1/Nrf2 signaling. The results revealed that oxymatrine attenuated ox-LDL-induced cytotoxicity and apoptosis via inhibiting NLRP3 inflammasome-mediated pyroptosis. In addition, the SIRT1/Nrf2 signaling pathway mediated the protective effects of oxymatrine against ox-LDL injury and oxidative stress, and contributed to the inhibitory effect of oxymatrine on ox-LDL-induced NLRP3 inflammasome-mediated pyroptosis in HUVECs. These results indicated that oxymatrine may mitigate ox-LDL injury by inhibiting NLRP3 inflammasome-mediated pyroptosis via activating the Sirt1/Nrf2 signaling pathway, providing new insight into a functional role of oxymatrine in atherosclerosis.
Atherosclerosis is a multifactorial, chronic inflammatory disease that causes injury of endothelial cells, which are the first to be exposed to the effects of stimuli and physiological factors from their surroundings (2,36). Oxymatrine, a natural bioactive compound, has been demonstrated to possess anti-inflammatory, antioxidant, antiviral, antifibrotic and cardioprotective properties (19,37). Recently, Zhang et al (19) revealed that oxymatrine may inhibit homocysteine-induced endothelial dysfunction, a hallmark of atherosclerosis, by attenuating autophagy-activated HUVEC apoptosis/death. Another study also demonstrated that oxymatrine prevented homocysteine-induced endothelial injury via attenuating mitochondrial-dependent apoptosis (18). Consistent with these studies, the present study also demonstrated that oxymatrine alleviated ox-LDL-induced cytotoxicity and apoptosis in HUVECs. Bcl-2 family proteins are involved in the mitochondria-dependent apoptosis pathway (38). The results of the present study further demonstrated that oxymatrine reversed the ox-LDL-induced increase in Bax expression and the decrease in Bcl-2 expression in HUVECs. Collectively, these results indicated that oxymatrine protected HUVECs against ox-LDL injury via inhibiting the mitochondria-dependent apoptosis pathway.
Inflammation is an important driver of atherosclerosis, and therapeutic targeting of inflammatory pathways is suggested to ameliorate atherosclerosis (39,40). NLRP3 inflammasomes are intracellular complexes involved in the inflammatory response that increases the levels of mature IL-1β and IL-18 and initiates pyroptosis via cleaving procaspase-1, playing a decisive role in atherosclerosis (3,41). A number of studies have revealed that oxymatrine plays a markedly beneficial role in multiple diseases due to its profound anti-inflammatory effects (37,42,43). However, to the best of our knowledge, the impact of oxymatrine on NLRP3 inflammasomes and pyroptosis has not been explored in atherosclerosis. The present study demonstrated that oxymatrine significantly inhibited the ox-LDL-induced increase in the expression of NLRP3 and ASC, as well as the expression of cleaved caspase-1, IL-1β and IL-18 in HUVECs, indicating that oxymatrine attenuated NLRP3 inflammasome activation-mediated pyroptosis under conditions of ox-LDL stimulation. In addition, the results further revealed that inhibition of NLRP3 inflammasome activation-mediated pyroptosis induced by siNLRP3 transfection inhibited the protective effects of oxymatrine against ox-LDL-induced HUVEC injury. Collectively, these results indicated that oxymatrine protected HUVECs against ox-LD-induced injury via inhibiting NLRP3 inflammasome-mediated pyroptosis.
SIRT1 was previously revealed to be involved in multiple cellular processes, and exerts marked protective effects against endothelial cell injury, vascular aging, inflammation and atherosclerotic plaque development (22,23). It was recently reported that oxymatrine plays a beneficial role in ischemic stroke (29) and non-alcoholic fatty liver disease (29) via promoting SRT1 signaling. However, the effect of oxymatrine on the SIRT1 signaling pathway in atherosclerosis remains unknown. In the present study, the results revealed that oxymatrine pretreatment markedly increased the expression of SIRT1 in ox-LDL-treated HUVECs. Nrf2, a key antioxidant sensor for cellular defense mechanisms, is considered as an important downstream target of SIRT1 and increases resistance to oxidative damage; it is also closely associated with atherosclerosis development (25). Under normal conditions, Nrf2 binds to its negative regulator, Kelch-like ECH-associated protein 1 (Keap1), which leads to its degradation via ubiquitination. In response to oxidative stress, Nrf2 is dissociated from Keap1 and translocates to the nucleus, thereby activating antioxidant responses by upregulating the expression of several antioxidant enzymes, particularly HO-1 (44,45). Consistent with these previous findings, the present study demonstrated that ox-LDL increased the expression of C-Nrf2, decreased the expression of N-Nrf2, and enhanced the expression of HO-1, which were also attenuated by oxymatrine. These results indicated that oxymatrine activated the SIRT1/Nrf2 signaling pathway in ox-LDL-treated HUVECs. Furthermore, the present study demonstrated that inhibition of SIRT1/Nrf2 signaling induced by siSIRT1 transfection mitigated the protective effects of oxymatrine against ox-LDL injury in HUVECs. Collectively, these results indicated that the SIRT1/Nrf2 signaling pathway mediated the cardioprotective function of oxymatrine in atherosclerosis.
An increasing number of studies have revealed that the mechanisms underlying the protective effects of oxymatrine are mainly associated with its anti-inflammatory and antioxidant properties (13,37,42). Similarly, the present results demonstrated that oxymatrine markedly attenuated ox-LDL-induced oxidative stress, as evidenced by the decrease in ROS generation and MDA content, and the increase in MMP and the activities of the antioxidant enzymes SOD, CAT and GSH-Px in HUVECs. Previous studies have reported that SIRT1/Nrf2 signaling dysregulation is involved in the ability to scavenge oxygen free radicals through the action of SOD, CAT and GSH-Px (46,47). Consistent with these studies, the present results further revealed that the inhibition of SIRT1/Nrf2 signaling pathway through siSIRT1 transfection eliminated the antioxidant properties of oxymatrine under conditions of ox-LDL stimulation. These results indicated that oxymatrine attenuated ox-LDL-induced injury by attenuating oxidative stress via promoting SIRT1/Nrf2 signaling in HUVECs.
An increasing number of studies have reported that activating SIRT1/Nrf2 signaling may reduce NLRP3 inflammasome activation (48) and pyroptosis (49). However, the role of the SIRT1/Nrf2 signaling pathway in the inhibitory effect of oxymatrine on NLRP3 inflammasome-mediated pyroptosis has not been reported to date. In the present study, the results demonstrated that siSIRT1 also abolished the inhibitory effect of oxymatrine on NLRP3 inflammasome-mediated pyroptosis, which was similar to the findings of previous studies reporting that NLRP3 inflammasome activation was associated with Nrf2 and SIRT1 activation and can be reversed by Nrf2 siRNA and SIRT1 inhibitor treatment (48,50). These results indicated that oxymatrine reduced NLRP3 inflammasome-mediated pyroptosis via enhancing SIRT1/Nrf2 signaling in ox-LDL-treated HUVECs.
Some shortcomings must be acknowledged in this study. Firstly, no specific immunofluorescence markers for pyroptosis in HUVECs were tested. Secondly, our experiments are limited to HUVECs, other cells or an in vivo experimental model have not yet been considered.
Collectively, the findings of the present study demonstrated that oxymatrine protected HUVECs against ox-LDL-induced injury by inhibiting NLRP3 inflammasome-mediated pyroptosis via activation of the SIRT1/Nrf2 signaling pathway. The results indicated that oxymatrine may serve as a potential therapeutic candidate for the treatment of atherosclerosis, and suggested a new strategy for targeting SIRT1/Nrf2 signaling and NLRP3 inflammasome-mediated pyroptosis.
Availability of data and materials
The datasets generated and/or analyzed in the present study are available from the corresponding author upon reasonable request.
Authors’ contributions
XJ and BW conceived, designed the experiments, and confirmed the authenticity of the raw data. XJ, WF and JZ performed the experiments and analyzed the data. NS and YY collected and analyzed the data. XJ, YY and BW wrote and revised the manuscript. All the authors have read and approved the final manuscript.
Ethics approval and consent to participate
Not applicable.
Patient consent for publication
Not applicable.
Competing interests
The authors declare that they have no competing interests.
Acknowledgments
Not applicable.
Funding
The present study was supported by the Hunan Provincial Natural Science Foundation of China (grant nos. 2018JJ3465 and 2019JJ50551).
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