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Introduction

Prostate cancer is the second leading cause of cancer-related mortality in American males (1). The androgen receptor (AR) is a ligand activated steroid hormone receptor and a key regulator of normal prostate development and function (2). The AR has a critical role in prostate cancer development and progression (3). Consequently, the current therapeutic strategies for prostate cancer intervention, including androgen ablation therapy, inhibits AR function (4). An aggressive form of prostate cancer therapy is based on a combination of androgen synthesis suppression and AR inhibition (5). Therefore, identification of chemical agents that inhibit AR signaling by known or novel mechanisms warrant further investigation for the development of a novel prostate cancer therapeutic approach.

Curcumin is a non-nutritive yellow pigment found in the spice turmeric, which is derived from the rhizome of the plant Curcuma longa Linn. Numerous studies have demonstrated the anticancer activity of curcumin and curcumin analogues in animal models (6–11), as well as growth inhibition and apoptosis-inductive effects in a variety of cancer cell lines in vitro (12–20). However, the clinical efficacy of curcumin is limited, which is likely due to its low bioavailability (21–23).

Our previous study reported on the synthesis and evaluation of 61 curcumin-related compounds for the inhibitory effects on cultured prostate cancer PC-3 cells, pancreas cancer Panc-1 cells and colon cancer HT-29 cells (24). Five of these curcumin analogues with different linker groups but identical symmetrical aromatic rings (as revealed in Fig. 1) were selected for further study. These compounds included (2E,6E)-2,6-bis(3,4,5-trimethoxybenzylidene) cyclohexanone (A10), (2E,5E)-2,5-bis(3,4,5-trimethoxybenzylidene) cyclopentanone (B10), (1E,4E)-1,5-bis(3,4,5-trimethoxyphenyl) penta-1,4-dien-3-one (C10), (3E,5E)-3,5-bis (3,4,5-trimethoxybenzyli-dene) tetrahydropyran-4-one (E10), (3E,5E)-3,5-bis (3,4,5-trimethoxybenzylidene) tetrahydrothiopyran-4-one (F10). Compounds with a heteroatom linker (compounds E10 and F10) demonstrated a stronger inhibitory effect than those without a heteroatom linker (compounds A10, B10 and C10) on the growth of human prostate cancer cells, although C10 had intermediate activity. It was also demonstrated that E10 and F10 more potently inhibited AR activity and testosterone (TT)- or dihydrotestosterone (DHT)-induced prostate specific antigen expression than A10, B10 and curcumin in CWR-22Rv1 cells.

Figure 1 Structures of curcumin and its analogues.

Materials and methods

Chemistry

Several curcumin analogues (A10, B10, C10, E10, F10 as demonstrated in Fig. 1) with different linker groups were synthesized by coupling the appropriate substituted benzaldehyde with cyclohexanone, cyclopentanone, acetone, tetrahydropyran-4-ones or tetrahydrothiopyran-4-one as previously described (24). Characterization of the compounds, (2E,6E)-2,6-bis(3,4,5-trimethoxy-benzylidene) cyclohexanone (A10), (2E,5E)-2,5-bis(3,4,5-trimethoxybenzylidene) cyclopentanone (B10), (1E,4E)-1,5-bis(3,4,5-trimethoxyphenyl) penta-1,4-dien-3-one (C10), (3E,5E)-3,5-bis(3,4,5-trime thoxybenzylidene) tetrahydropyran-4-one (E10) and (3E,5E)-3,5-bis(3,4,5-trime-thoxybenzylidene) tetrahydro thiopyran-4-one (F10), was previously described in detail (24).

Cell culture and reagents

CWR-22Rv1 and LNCaP cells were obtained from the American Type Culture Collection (ATCC; Rockville, MD, USA). RPMI-1640 tissue culture medium, penicillin-streptomycin, L-glutamine and fetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA). CWR-22Rv1 cells were maintained in RPMI-1640 culture medium. RPMI-1640 medium was supplemented with 10% FBS, penicillin (100 units/ml)-streptomycin (100 μg/ml) and L-glutamine (300 μg/ml). The cultured cells were grown at 37°C in a humidified atmosphere of 5% CO2 and were passaged twice a week. Curcumin analogues were dissolved in dimethyl sulfoxide and the final concentration of DMSO was 0.1% in all experiments.

MTT, trypan blue and apoptosis assays

For the MTT assay, CWR-22Rv1 and LNCaP cells were seeded at a density of 2×104 cells/ml of medium in a 96-well plate (0.2 ml/well) and incubated for 24 h. The cells were then treated with various concentrations (0.5–30 μM) of curcumin analogues for 72 h. Following treatment, 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide was added to each well of the plate and incubated for 1 h. After careful removal of the medium, 0.1 ml DMSO was added to each well and absorbance at 550 nm was recorded on a microplate reader. For the trypan blue exclusion assay, the CWR-22Rv1 cells were seeded at a density of 2×104 cells/ml of medium in 35 mm tissue culture dishes and incubated for 24 h. The cells were then treated with curcumin analogues for 96 h. The number of viable cells following each treatment was determined using a hemocytometer under a light microscope (Nikon Optiphot; Nikon, Tokyo, Japan). The cell viability was determined by the trypan blue exclusion assay, which was performed by mixing 80 μl of the cell suspension and 20 μl of 0.4% trypan blue stain solution for 2 min. The blue cells were counted as dead cells and the cells that did not absorb dye were counted as live cells. Apoptosis was determined by morphological assessment in the cells stained with propidium iodide (25). Apoptotic cells were identified by classical morphological features, including nuclear condensation, cell shrinkage and the formation of apoptotic bodies. At least 200 cells were counted in each sample and the percentage of apoptotic cells was determined.

AR luciferase reporter assay

AR transcriptional activity was measured by an AR-luciferase reporter gene expression assay. An AR luciferase construct was stably transfected into CWR-22Rv1 cells and a single stable clone, CWR-22Rv1/AR, was used in the present study. CWR22-Rv-1 cells cultured in 10% FBS RPMI-1640 medium were infected with a lentivirus carrying the Cignal Lenti AR reporter (luciferase; Qiagen, Valencia, CA, USA) in the medium containing 8 μg/ml Polybrene (Sigma, St. Louis, MO, USA). At 6 h following infection, the culture medium was replaced with fresh 10% RPMI-1640 medium. To establish the cells expressing stable AR-luciferase reporter, cells were selected using puromycin (5 μg/ml) on day 3 following infection for one week. The selected cells were then used for the reporter assay for AR activity.

The CWR-22Rv1/AR cells were treated with curcumin and its analogues for 24 h, and the luciferase activities were measured using luciferase assay kits from Promega Corporation (Madison, WI, USA). Following treatment, the cells were washed with ice-cold phosphate-buffered saline (PBS) and harvested in a reporter lysis buffer. After centrifugation, 10 μl aliquots of the supernatants were used for measuring the luciferase activity with a luminometer from Turner Designs Instruments (Sunnyvale, CA, USA). The luciferase activity was normalized against protein concentration and expressed as the percentage of luciferase activity in the control cells, which were treated with DMSO solvent. The protein level was determined by Bio-Rad protein assay kits (Bio-Rad, Hercules, CA, USA) according to the manufacturer’s instructions.

Western blot analysis

Following treatment with curcumin, A10, B10, C10, E10 and F10 for 24 h, the CWR-22Rv1 and LNCaP cells were washed with ice-cold PBS and lysed with 800 μl of lysis buffer (10 mm Tris-HCl, pH 8.0, 10 mm EDTA, 150 mm sodium chloride, 1% NP-40, 0.5% SDS, in deionized water). The homogenates were centrifuged at 12,000 × g for 15 min at 4°C. The protein concentration of whole cell lysates was determined with a Bio-Rad protein assay kit (Bio-Rad). Equal amounts (50 μg) of protein were then resolved on a 10% Criterion Precast Gel (Bio-Rad) and transferred onto a PVDF membrane using a semi-dry transfer system. The membrane was then probed with anti-PSA (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) primary antibody. Following hybridization with primary antibody, the membrane was washed with Tris-buffered saline (TBS) three times, then incubated with horseradish peroxidase-conjugated secondary antibody (Santa Cruz Biotechnology, Inc.) and washed with TBS three times. Final detection was performed with enhanced chemiluminescent reagents. The extent of protein loading was determined by blotting for β-actin. The membrane was incubated in stripping buffer (100 mm β-mercaptoethanol, 2% SDS and 62.5 mm Tris-HCl at pH 6.7) at 50°C for 30 min with occasional agitation prior to incubating in blocking buffer and re-probing using anti-β-actin (Santa Cruz Biotechnology, Inc.).

Statistical analyses

The analyses of differences among curcumin and its analogues on the TT- or DHT-induced activation of AR were based on a repeated measurement model. The effects of the treatments were assessed by comparing the rates of change over time between the treatment groups (i.e., comparing the slopes between the treatment groups). The analysis of variance (ANOVA) method with the Tukey-Kramer test was used for the comparison of effects among the different treatment groups at the end of the study. P<0.05 was considered to indicate a statistically significant difference.

Results

Effects of curcumin and its analogues on CWR-22Rv1 and LNCaP cells

The inhibitory effects of curcumin analogues on the growth of cultured CWR-22Rv1 and LNCaP cells were determined by using MTT and trypan blue exclusion assays. For each incubation, curcumin was examined as a positive control. The inhibitory effects of different concentrations of curcumin and its analogues in cultured CWR-22Rv1 and LNCaP cells are presented in Fig. 2. All of the compounds had stronger inhibitory effects than curcumin as determined by the MTT assay. Among the five curcumin analogues tested in the present study, compounds E10, F10 and C10 exhibited the most potent inhibitory effects on the growth of cultured CWR-22Rv1 and LNCaP cells. The IC50 values for E10 and F10 were lower than 1 μM in the CWR-22Rv1 and LNCaP cells, indicating that these compounds were ~20-fold more active than curcumin (IC50=16.99 μM). As demonstrated in Table I, the IC50 values of the five curcumin analogues ranged from 0.82 to 13.62 μM. The numbers of viable and dead cells were determined by the trypan blue exclusion assay following treatment of the CWR-22Rv1 cells with curcumin and its analogues for 96 h. As demonstrated in Table II, a reduction in the number of viable cells were observed. Compared with the control group, the numbers of viable cells in the various groups treated with curcumin and its analogues were decreased by 15.4% to 95.4% (Table II). The effects of curcumin and its analogues on apoptosis of CWR-22Rv1 cells were determined by morphological assessment in the propidium iodide stained cells. In these studies, the CWR-22Rv1 cells were treated with curcumin and its analogues for 96 h. As demonstrated in Table II, weak inhibitory effects on growth and weak stimulatory effects on the induction of apoptosis of CWR-22Rv1 cells were observed by treatment with A10 (1 μM) and B10 (1 μM), while more evident effects were observed by treatment with E10 (0.5 μM) and F10 (0.5 μM). C10 (1 μM) had a moderate inhibitory effect on growth and a moderate stimulatory effect on apoptosis.

Figure 2 Inhibitory effects of curcumin analogues on the growth of (A) CWR-22Rv1 and (B) LNCaP cells. Prostate cancer CWR-22Rv1 and LNCaP cells were seeded at a density of 2×104 cells/ml of medium in 96-well plates (0.2 ml/well) and incubated for 24 h. The cells were then treated with various concentrations (0.2–30 μM) of the different compounds for 72 h. (A and B) The effects of the different compounds on the growth of CWR-22Rv1 and LNCaP cells were determined by the MTT assay. Each value represents the mean ± standard error from three separate experiments.

Table I Inhibitory effects of curcumin and its analogues on the growth of CWR-22Rv1 cells.

Table I

Inhibitory effects of curcumin and its analogues on the growth of CWR-22Rv1 cells.

	IC50 (μM)
Compound	CWR-22Rv1	LNCaP
Curcumin	16.99±3.0	13.59±1.8
A10	8.76±0.5	9.8±1.2
B10	13.62±2.0	11.4±1.5
C10	1.78±0.2	1.07±0.2
E10	0.82±0.1	0.65±0.2
F10	0.96±0.1	0.81±0.2

[i] Prostate cancer CWR-22Rv1 cells were seeded at a density of 2×10⁴ cells/ml of medium in 96-well plates (0.2 ml/well) and incubated for 24 h. The cells were then treated with various concentrations (0.1–20 μM) of the different compounds for 72 h. The effects of the different compounds on the growth of CWR-22Rv1 cells were determined by an MTT assay. Each value represents the mean ± standard error from three separate experiments.

Table II Effects of curcumin and its analogues on the growth of CWR-22Rv1 cells.

Table II

Effects of curcumin and its analogues on the growth of CWR-22Rv1 cells.

Treatment	No. of viable cells (1×10−4)	Percent apoptotic cells
Control	43.1±1.4	1.7±0.2
Curcumin	36.4±1.5	2.3±0.3
A10	30.5±1.3	4.7±0.9
B10	33.0±1.0	3.1±0.3
C10	12.4±0.9	7.6±0.5
E10	8.5±0.8	22.8±1.0
F10	2.0±0.3	29.4±2.1

[i] Prostate cancer CWR-22Rv1 cells were seeded at a density of 2×10⁴ cells/ml in 35 mm tissue culture dishes and incubated for 24 h. The cells were then treated with curcumin and its analogues for 96 h (curcumin, A10, B10 and C10 at 1 μM; E10 and F10 at 0.5 μM). The number of viable cells was determined by a trypan blue exclusion assay. Apoptotic cells were determined by morphological assessment. Each value represents the mean ± standard error from three separate experiments.

Effects of curcumin and its analogues on AR activity in CWR-22Rv1/AR cells

An AR-luciferase reporter gene expression assay in CWR-22Rv1/AR cells was utilized to determine the effect of curcumin and its analogues on the TT- or DHT-induced activation of AR. Cultured CWR-22Rv1/AR cells were treated with TT in combination with curcumin analogues (1 μM) for 24 h. As demonstrated in Fig. 3, a marginal inhibitory effect on the TT- or DHT-induced increase in AR activity was observed in the cultured CWR-22Rv1/AR cells treated with curcumin (1 μM), A10 (1 μM) or B10 (1 μM), while more evident inhibitory effects were observed in the CWR-22Rv1/AR cells treated with C10 (1 μM), E10 (1 μM) and F10 (1 μM). Statistical analysis using ANOVA with the Tukey’s multiple comparison tests demonstrated that AR activity was significantly lower in the cells treated with group C10, E10 and F10 compounds than in the cells treated with curcumin or A10 and B10. P<0.001 for control vs. TT (DHT) + CUR, TT (DHT) + A10 or TT (DHT) + B10; P<0.001 for TT (DHT) vs. TT (DHT) + A10, TT (DHT) + B10, TT (DHT) + C10, TT (DHT) + E10 or TT (DHT) + F10; P<0.001 for TT (DHT) + CUR vs. TT (DHT) + C10, TT (DHT) + E10 or TT (DHT) + F10; P<0.001 for TT (DHT) + A10 vs. TT (DHT) + C10, TT (DHT) + E10 or TT (DHT) + F10; P<0.001 for TT (DHT) + B10 vs. TT (DHT) + C10, TT (DHT) + E10 or TT (DHT) + F10.

Figure 3 Effect of curcumin analogues on TT- or DHT-induced increase in androgen receptor reporter activity in CWR-22Rv1/AR cells: (A) for TT and (B) for DHT. The CWR-22Rv1/AR cells were seeded at a density of 1×104 cells/ml of medium for 24 h. The medium was then changed to RPMI without fetal bovine serum, and the cells were treated with vehicle (control) or with TT (100 nm) or DHT (10 nm) alone or in combination with curcumin and curcumin analogues (1 μM) for 24 h. The luciferase activity and protein concentration of the CWR-22Rv1/AR cells were measured. Each value represents the mean ± standard error from three separate experiments. TT, testosterone; DHT, dihydrotestosterone; CUR, curcumin.

Effects of curcumin and its analogues on the expression of PSA in CWR-22Rv1 and LNCaP cells

The levels of PSA were evaluated by western blot analysis using an anti-PSA antibody. The cultured CWR-22Rv1 and LNCaP cells were treated with TT, DHT and curcumin analogues A10, B10, C10, E10 or F10 for 24 h, and the expression of PSA was analyzed by western blotting. As demonstrated in Fig. 4, treatment of CWR-22Rv1 and LNCaP cells with F10 and E10 resulted in a marked decrease in the level of PSA while the other compounds (curcumin, A10, B10 and C10) were less active. The results indicate that the effects of E10, F10, A10 and C10 on CWR-22Rv1 and LNCaP cells were all associated with a decrease in PSA in TT- and DHT-induced cells. B10 was inactive.

Figure 4 Effect of curcumin and its analogues on TT- and DHT-induced increase in PSA formation in the CWR-22Rv1 cells. (A) TT and (B) DHT. Effect of curcumin and its analogues on the activation of AR in CWR-22Rv1 and LNCaP cells. (C) CWR-22Rv1 and (D) LNCaP. The CWR-22Rv1 and LNCaP cells were seeded at a density of 1×104 cells/ml of medium in 100 mm culture dishes (10 ml/dish) and incubated for 24 h. The medium was changed to RPMI without fetal bovine serum, and the cells were then treated with vehicle, (A) 100 nm TT or (B) 10 nm alone or together with 1 μM curcumin, A10, B10, C10, E10 or F10 for 24 h. PSA was determined by western blot analysis with anti-PSA antibody. The extent of protein loading was determined by blotting for β-actin, and the levels of PSA in the western blots were analyzed by optical density measurements and normalized for β-actin to obtain the RD for the various samples. Representative blots from three experiments are demonstrated. TT, testosterone; DHT, dihydrotestosterone; PSA, prostate specific antigen; RD, relative optical density; CON, control; CUR, curcumin; AR, androgen receptor.

Discussion

In the present study, it was identified that several curcumin analogues (A10, B10, C10, E10 and F10) exhibited stronger anticancer activities than curcumin in cultured human prostate cancer CWR-22Rv1 and LNCaP cells. Among the curcumin analogues, compounds F10 and E10 demonstrated a more potent inhibitory effect on the growth of CWR-22Rv1 and LNCaP cells than any of the other curcumin analogues, and they also had higher stimulatory effects on apoptosis in CWR-22Rv1 cells compared with the other compounds (Fig. 5). In addition, it was identified that all curcumin analogues examined (except for B10) were more potent inhibitors of AR in LNCaP and CWR-22Rv1 cells than curcumin. E10 and F10 were the most potent compounds among the five curcumin analogues examined for inhibiting the activation of AR.

Figure 5 Effects of curcumin and its analogues on apoptosis of prostate cancer CWR-22Rv1 cells. Human prostate cancer CWR-22Rv1 cells were seeded at a density of 2×104 cells/ml in 35 mm tissue culture dishes and incubated for 24 h. The cells were then treated with curcumin and its analogues (curcumin, A10, B10 and C10 at 1 μM; E10 and F10 at 0.5 μM). Apoptotic cells were determined by morphological assessment. Representative micrographs of (A) propidium iodide-stained control and (B) E10 at 0.5 μM treated CWR-22Rv1 cells.

The natural product curcumin (diferuloylmethane) has been demonstrated to inhibit numerous targets in prostate epithelial cells with an importance in cancer formation and progression. Among these targets are transcription factors, receptors, intracellular kinases, cytokines and growth factors (26). The effect of curcumin on the AR and on its target PSA has been demonstrated by several independent investigators using both endogenously expressed AR in LNCaP cells and ectopically expressed AR in PC-3 cells (27,28). However, in these studies, curcumin was used at relatively high concentrations, typically at >20 μM. It has previously been reported that curcumin has poor bioavailability, which has been determined in animal models and humans (29). This limitation has led researchers to generate a variety of synthetic analogues of curcumin and to investigate their capability to affect a number of molecular pathways implicated in tumorigenesis and cancer progression (30–33). Typical structure modifications include the introduction of substituents on the phenyl rings and modifications of the length of the linker between the phenyl rings. A specific group of such analogues has been exploited for their ability to inhibit AR function (34), and a number of these agents have been demonstrated to inhibit the expression of AR (35).

The present study determined the inhibitory effects of different curcumin analogues on TT- and DHT-induced activation of AR in CWR-22Rv1 cells. The results indicate that the different curcumin analogues had a similar effect on TT- or DHT-induced AR activation.

Based on the analysis of the association between the structures of curcumin-related compounds and their ability to inhibit the growth of cultured cancer cells, the presence of groups on the linker between the same aromatic rings was found to have a key role in determining the anticancer activity of the various analogues. Among the different series of curcumin-related compounds, linear or cyclic linkers between the two aromatic rings of curcumin-related compounds demonstrated different activity trends. In general, the compounds with a tetrahydrothiopyran-4-one (F10) or a tetrahydropyran-4-one (E10) linker exhibited the strongest activity, and compounds with an acetone linker (C10) exhibited moderate activity whereas compounds with a cyclohexanone linker (A10) or a cyclopentanone linker (B10), were less active. The activities of compounds with a heteroatom linker (E10 and F10) demonstrated improved effects compared with those without a heteroatom linker (A10, B10 and C10), which suggests that flexibility of curcumin-like compounds may enhance their antitumor activities by having interactions with the DNA of cancer cells and disrupting the activity of transcription factors, such as AR. Compounds with a small and highly rigid linker should be less active as previously described (36,37). The results indicate that one of the potential mechanisms for the anticancer effect of curcumin analogues was inhibition of AR pathways in human prostate cancer cells.

Acknowledgements

The present study was supported by the 2011 Guangdong Province Leadership Grant, China National Science Foundation Grants (grant no. 81272452 and 21272043), and by department funds from the Department of Chemical Biology in the Ernest Mario School of Pharmacy at Rutgers University (Piscataway, NJ, USA).

References